Swim Training Improves HOMA-IR in Type 2 Diabetes Induced by High Fat Diet and Low Dose of Streptozotocin in Male Rats

Ghiasi, Rafighe; Soufi, Farhad Ghadiri; Somi, Mohammad Hossein; Mohaddes, Gisou; Bavil, Fariba Mirzaie; Naderi, Roya; Alipour, Mohammad Reza

doi:10.15171/apb.2015.052

Cited by 38 publications

(29 citation statements)

References 40 publications

(58 reference statements)

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“…In our study, HDL-C was significant low in NTD group. This finding is consistent with Ghiasi R, et al who induced T2DM by STZ-high fat diet [35]. Assembly of all factors above confirms the use of this induced animal model as a model of T2DM.…”

Section: Detection Of Ir By Elisasupporting

confidence: 91%

Histopathological Alteration in STZ-Nicotinamide Diabetic Rats, a Complication of Diabetes or a Toxicity of STZ?

Mh¹,

Imt²,

A³

et al. 2018

Int J Diabetes Clin Res

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Background: Type 2 diabetes mellitus (T2DM) previously known as non-insulin dependent diabetes mellitus is the most common type of diabetes mellitus. The rapid increasing prevalence, high morbidity and mortality of the disease must be encountered by an increase in scientific research. Animal models are very important in the preclinical studies to validate the use of new drugs. Streptozotocin-nicotinamide (STZ-NA) is used to induce T2DM in animals. Aim: The aim of this study was to assess STZ-NA as a model of T2DM and to investigate the causative factors that lead to hepatic histopathological changes. Methodology: Twenty-five male Wistar rats were divided into four groups. One normal non-diabetic control group (NNC) (n = 6), two diabetic groups subdivided into non-treated diabetic group (NTD) (n = 6) and metformin treated diabetic group (MTD) (n = 7) and the fourth group was composed of rats failed to develop diabetes [failed induction group (FIG)] (n = 6). Diabetes was induced by a single intraperitoneal injection of STZ (65 mg/kg-bw) 15 min after intraperitoneal administration of nicotinamide (120 mg/kgbw). Induction of diabetes was confirmed after 72 hours by fasting blood glucose (FBG) ≥ 250 mg/dl. 8 weeks after induction, oral glucose tolerance test was performed; blood samples were taken for analysis of lipid profile, urea, creatinine and hepatic enzymes; samples from the liver were prepared for histopathological study and samples from the skeletal muscles were taken for determination of insulin receptor content by Elisa. Results: Metformin improved glucose intolerance. Serum high density lipoprotein cholesterol (HDL-C) was significantly reduced in the NTD group compared with the NNC group (P value 0.006) whereas no significant difference in low density lipoprotein cholesterol and triglycerides detected. Skeletal muscle insulin receptor was reduced in both diabetic groups yet, reduction was significant in MTD group compared with NTD group [P values were (0.01) and (0.06) in the MTD and NTD respectively]. Hepatic enzymes, urea and creatinine were within normal range. Histopathological study revealed hepatic histopathological alterations which were more obvious in NTD compared with MTD and FIG. Conclusion: Our study showed that STZ-NA is a good model for T2DM regarding hyperglycemia, response to metformin, dyslipidemia and the histopathological changes. In addition, it emphasized that hepatic hydropic degeneration of the hepatocytes was a consequence of diabetes rather than STZ.

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Section: Detection Of Ir By Elisasupporting

confidence: 91%

Histopathological Alteration in STZ-Nicotinamide Diabetic Rats, a Complication of Diabetes or a Toxicity of STZ?

Mh¹,

Imt²,

A³

et al. 2018

Int J Diabetes Clin Res

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show abstract

“…The selected rats were randomly divided into control group (n=12) and study group (n=100). The rats in the control group were fed with normal standard diet, while the rats in the study group were fed with high fat, high sugar and high protein diet (HFSPD, food ratio: carbohydrate 25%, protein 15.2%, fat (lard) 58.8%, and sodium cholate 1% ( 31 , 32 ). The body weights of rats were weighed every two weeks.…”

Section: Methodsmentioning

confidence: 99%

Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment

Tang

et al. 2017

Int J Mol Med

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Lychee seed is a traditional Chinese medicine and has many beneficial effects such as modulation of blood sugar and lipids, antioxidation, antivirus and antitumor. Studies have indicated that type II diabetes mellitus (T2DM) and Alzheimer's disease (AD) share common biological mechanisms including insulin resistance, impaired glucose metabolism, β-amyloid (Aβ) formation, oxidative stress and presence of advanced glycation end products (AGEs). The present study investigated the effects of lychee seed extract (LSE) on neuroprotection, cognitive function improvement and possible underlying mechanisms in a rat model of T2DM with cognitive impairment. We analyzed the chemical profile of LSE using a UHPLC-SPD chromatogram and evaluated its effect on the improvement of spatial learning and memory of rats by a Morris water maze. The levels of glucose, insulin, Aβ, AGEs, Tau protein and acetylcholinesterase in the blood and/or hippocampus of rats were determined by blood-glucose meter, radioimmunoassay, chemical chromatometry, enzyme-linked immunosorbent assay (ELISA) and immunohistochemical analysis, respectively. Results demonstrated that LSE consists of eight major and around 20 minor ingredients, and it remarkably prevents neuronal injury and improves cognitive functions in T2DM rats. The levels of glucose, insulin, Aβ, AGEs and Tau protein were significantly increased in the blood and/or hippocampus of T2DM rats, while LSE remarkably decreased their levels compared to vehicle treatment (P<0.01). The possible mechanisms may be associated with IR improvement and decreased formations of Aβ, AGEs and Tau protein in the hippocampus of T2DM rats. LSE may be developed as the agent for the treatment of T2DM and/or AD clinically.

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“…The effects of physical exercise on the improvement of glycemic control in diabetic patients (decrease in HbA1c and fasting glucose) are frequently reported [32,33,34]. Generally, this effect is due to the increased glucose uptake by skeletal muscle during exercise and increased insulin sensitivity for some hours after physical activity [35].…”

Section: Discussionmentioning

confidence: 99%

Exercise and Beta-Glucan Consumption (Saccharomyces cerevisiae) Improve the Metabolic Profile and Reduce the Atherogenic Index in Type 2 Diabetic Rats (HFD/STZ)

et al. 2016

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Physical activity and the ingestion of dietary fiber are non-drug alternatives commonly used as adjuvants to glycemic control in diabetic individuals. Among these fibers, we can highlight beta-glucans. However, few studies have compared isolated and synergic effects of physical exercise and beta-glucan ingestion, especially in type 2 diabetic rats. Therefore, we evaluated the effects beta-glucan (Saccharomyces cerevisiae) consumption, associated or not to exercise, on metabolic parameters of diabetic Wistar rats. The diabetes mellitus (DM) was induced by high-fat diet (HFD) associated with a low dose of streptozotocin (STZ—35 mg/kg). Trained groups were submitted to eight weeks of exercise in aquatic environment. In the last 28 days of experiment, animals received 30 mg/kg/day of beta-glucan by gavage. Isolated use of beta-glucan decreased glucose levels in fasting, Glycated hemoglobin (HbA1c), triglycerides (TAG), total cholesterol (TC), low-density lipoprotein (LDL-C), the atherogenic index of plasma. Exercise alone also decreased blood glucose levels, HbA1c, and renal lesions. An additive effect for reducing the atherogenic index of plasma and renal lesions was observed when both treatments were combined. It was concluded that both beta-glucan and exercise improved metabolic parameters in type 2 (HFD/STZ) diabetic rats.

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Swim Training Improves HOMA-IR in Type 2 Diabetes Induced by High Fat Diet and Low Dose of Streptozotocin in Male Rats

Cited by 38 publications

References 40 publications

Histopathological Alteration in STZ-Nicotinamide Diabetic Rats, a Complication of Diabetes or a Toxicity of STZ?

Histopathological Alteration in STZ-Nicotinamide Diabetic Rats, a Complication of Diabetes or a Toxicity of STZ?

Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment

Exercise and Beta-Glucan Consumption (Saccharomyces cerevisiae) Improve the Metabolic Profile and Reduce the Atherogenic Index in Type 2 Diabetic Rats (HFD/STZ)

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