SWIM-Up Procedure in Boar Semen Improves Motility and Viability but Recovered Sperm Could Carry Active Caspases and Chromatin Damage

Aragon, Melissa; Salinas, Elizabeth Morales; Pescador, S.N.; Salazar, Glicella

doi:10.3923/javaa.2012.431.437

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…In our study, caspase‐3/7 activation in alpaca spermatozoa varied slightly during the first 2 h (25%–35%), increased significantly after 3 h of incubation, and increased again by the fourth hour (45%–50%). There are no similar reports in other species, however, the proportion of apoptotic sperm in fresh semen has been reported to be between 12% in pigs (Morales et al, 2012) and 19% in humans (Kotwicka et al, 2008). In llamas, viability in thawed spermatozoa was similar during the first 3 h of incubation; however, total and progressive motility decreased significantly at the third hour of incubation (Fumuso et al, 2019).…”

Section: Discussionmentioning

confidence: 96%

Activation of caspase‐3/7, an apoptotic‐related marker, during incubation and cryopreservation of alpaca (Vicugna pacos) spermatozoa

Segura,

La Rosa,

Báez

et al. 2023

Reprod Domestic Animals

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Caspases are crucial mediators of programmed cell death (apoptosis). Apoptosis can occur in spermatozoa during spermatogenesis or epididymal transit, as well as in ejaculated spermatozoa. A high proportion of apoptotic sperm would be a poor indicator of the freezability of a raw seminal sample. Alpaca spermatozoa are notoriously difficult to freeze successfully. Therefore, the objectives of this study were to study caspase activation during incubation (37°C) of fresh alpaca spermatozoa, as well as before and after cryopreservation, to gain some insight into the mechanisms behind the vulnerability of alpaca spermatozoa. Eleven sperm samples were incubated for 4 h at 37°C (Study 1), and 23 samples were frozen using an automated system (Study 2). Caspase‐3/7 activation was assessed at 0,1,2,3, and 4 h in samples incubated at 37°C (Study 1); and before/after cryopreservation (Study 2) using CellEvent™ Caspase 3/7 Green Detection Reagent and flow cytometry. The proportions of alpaca spermatozoa with caspase‐3/7 activated increased (p < 0.05) after 3–4 h of incubation at 37°C; however, caspase activation was similar before and after cryopreservation (36.2 ± 11.2% vs. 36.6 ± 33.7%, p > 0.05). The high standard deviation found after freezing could be explained by the existence of two subpopulations: one subpopulation where caspase‐3/7 activation decreased during cryopreservation (from 36.6 ± 9.1% to 1.5 ± 2.2%), and the other subpopulation where caspase‐3/7 activation increased after cryopreservation (from 37.7 ± 13.0% to 64.3 ± 16.7%). In conclusion, after 3–4 h of incubation, caspase‐3/7 activation increased in fresh alpaca sperm, whereas cryopreservation affects alpaca sperm samples in different ways.

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Section: Discussionmentioning

confidence: 96%

Activation of caspase‐3/7, an apoptotic‐related marker, during incubation and cryopreservation of alpaca (Vicugna pacos) spermatozoa

Segura,

La Rosa,

Báez

et al. 2023

Reprod Domestic Animals

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Sperm selection by Capripure® density-gradient centrifugation versus the dextran swim-up procedure in wild mountain ruminants

Santiago-Moreno

Esteso

Castaño

et al. 2014

Animal Reproduction Science

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Differences in preservation of canine chilled semen using simple sperm washing, single-layer centrifugation and modified swim-up preparation techniques

Dorado

Gálvez

Demyda-Peyrás

et al. 2016

Reprod. Fertil. Dev.

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This study compared the efficacy of simple sperm washing (SW), single-layer centrifugation (SLC) and modified swim-up (SU) techniques in the preparation of dog spermatozoa for cooling. Eighteen ejaculates, collected from three dogs (six per dog), were pooled (three ejaculates per pool) and divided into three aliquots: (1) one aliquot was washed and cooled at 5°C for 72h, considered as control (SW-control), (2) the second aliquot was selected by SLC through Androcoll-C and subsequently cooled in the same way as the SW-control samples (SLC-AC) and (3) the last aliquot was selected by a modified SU method with Androcoll-C and cooled as mentioned above (SU-AC). Assessment of sperm motility, sperm morphology, sperm membrane integrity and acrosome integrity were performed on aliquots of fresh semen and chilled-rewarmed samples. Sperm membrane integrity and progressive motility were significantly (PPP>0.05). The recovery rates were not significantly (P>0.05) different between SW-control, SLC-AC and SU-AC samples. Our results confirm that SU-AC may be a successful method for the preparation of dog spermatozoa for cooling.

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SWIM-Up Procedure in Boar Semen Improves Motility and Viability but Recovered Sperm Could Carry Active Caspases and Chromatin Damage

Cited by 4 publications

References 10 publications

Activation of caspase‐3/7, an apoptotic‐related marker, during incubation and cryopreservation of alpaca (Vicugna pacos) spermatozoa

Activation of caspase‐3/7, an apoptotic‐related marker, during incubation and cryopreservation of alpaca (Vicugna pacos) spermatozoa

Sperm selection by Capripure® density-gradient centrifugation versus the dextran swim-up procedure in wild mountain ruminants

Differences in preservation of canine chilled semen using simple sperm washing, single-layer centrifugation and modified swim-up preparation techniques

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