Swimming statistics of cargo-loaded single bacteria

Prakash, Praneet; Abdulla, Amith Z.; Singh, Varsha; Varma, Manoj M.

doi:10.1039/d0sm01066a

Cited by 6 publications

(9 citation statements)

References 33 publications

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“…An anomalous cargo transport regime was also predicted in biofilm forming bacteria Pseudomonas aeruginosa (PA14) 11,12 , where swimming is driven by multiple (on average two) rotating helical flagella (length~4 μ m) that can bundle to propel the bacterium in a corkscrew-like motion or unbundle to change direction, exhibiting a run-and-tumble swimming pattern 66 . This anomalous behavior is expected to exist for a hypothetical mutant of PA14 which has a larger (around three times) bacteria size in comparison to the wild type.…”

Section: Discussionmentioning

confidence: 88%

“…An anomalous cargo transport regime is also reported in Refs. [43, 44], where a cargo is propelled by rotation of the helical flagella of bacteria Pseudomonas aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

“…In the past decade, the sensory capabilities and the autonomous motility and power of various bacterial species, mainly E. coli have been extensively utilized as a biological micro-actuator in bio-hybrid micro-swimmers to provide an efficient cargo (e.g. liposomes or coated beads) transport 5–12 .…”

Section: Introductionmentioning

confidence: 99%

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Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii^†

Ahmad¹,

Bae

Su³

et al. 2021

Preprint

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Bio-actuated micro-swimmers provide a platform to understand physical principles related to the motion of micro-organisms at low Reynolds numbers. Here, we used isolated and demembranated flagella from green algae Chlamydomonas reinhardtii as an ATP-fueled bio-actuator for propulsion of micron-sized beads. Chlamydomonas flagella have an asymmetric waveform, which can be accurately described as a superposition of a static component corresponding to an arc-shaped intrinsic curvature, a mode describing the global oscillations of the axonemal curvature, and a main base- to-tip traveling wave component. By decomposing experimental beat patterns in Fourier modes, and applying resistive force theory, we performed numerical simulations and obtained analytical approximations for the mean rotational and translational velocities of a flagellum-propelled bead. Our analysis reveals the existence of a counter- intuitive anomalous propulsion regime where the speed of the flagellum-driven cargo increases with increasing the cargo size. Further, it demonstrates that in addition to the intrinsic curvature and even harmonics, asymmetric bead-flagellum attachment also contributes in the rotational velocity of the micro-swimmer. This turning mechanism induced by sideways cargo attachment has potential applications in fabrication of bio- actuated medical micro-robots in the subject of targeted drug delivery and synthetic micro-swimmers.

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Section: Discussionmentioning

confidence: 88%

“…An anomalous cargo transport regime is also reported in Refs. [43, 44], where a cargo is propelled by rotation of the helical flagella of bacteria Pseudomonas aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

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Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii^†

Ahmad¹,

Bae

Su³

et al. 2021

Preprint

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“…For comprehensive summaries of current work, we refer the reader to reviews covering recent progress in the physics of swimming microbes [16,17] and bioconvection [7]. This area of research is also closely related to active matter [18] comprising biological swimmers, as we have just described, but also synthetic [19] and biohybrid ones [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Photogyrotactic Concentration of a Population of Swimming Microalgae Across a Porous Layer

Prakash

Croze

2021

Front. Phys.

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The light environment controls the swimming of microalgae through a light-seeking and avoiding behaviour, which is known as phototaxis. In this work, we exploit phototaxis to control the migration and concentration of populations of the soil microalga Chlamydomonas reinhardtii. By imaging a suspension of these microalgae in a cuvette illuminated from above by blue light, we study how phototaxis changes the stability of the suspension and demonstrate how a thin, porous layer at the top of the cuvette prevents phototaxing microalgae from sinking, leading to the up-concentration of the microalgae in the region above the porous layer. We discuss the potential implications of our findings for microalgae in biotechnological applications and the natural environment.

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“…Active particles often move through complex media that are not simple Newtonian fluids, both in natural [19][20][21][22][23][24][25] and laboratory [26][27][28][29][30] environments, including many situations where geometrical confinement becomes important. For example, bacteria can have altered trajectories [31,32] and accumulate when swimming along boundaries [33][34][35][36], including in naturally arising situations such as oil drops [37][38][39], while engineered boundaries can be designed with complex shapes to guide and collect bacteria [40][41][42] and sperm [43][44][45][46] along them.…”

mentioning

confidence: 99%

Fore-aft clearance controls how three-dimensional confinement affects micropropulsion

Kamarapu¹,

Jabbarzadeh²,

Fu³

2022

Preprint

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Systems of active particles are often affected by confinement due to nearby boundaries. Recently, there has been interest in the effect of confinement by complex three dimensional geometries, as might occur in structured environments such as porous media, foams, gels, or biological tissues and ducts. The effects of confinement for particles moving along boundaries has been extensively studied, but in three dimensions active particles move not only parallel to boundaries, but also towards or away from boundaries. The consequences of this fore-aft clearance is less well understood. Swimmers that actively remodel their environment create an ideal situation to study the effect of clearance, since they maintain a steady clearance while translating. By numerically studying the locomotion of the bacterium Helicobacter pylori, which de-gels surrounding gastric mucus to make a comoving pocket of fluid around itself, we show that the effect of three-dimensional confinement is controlled by clearance, rather than distance from a parallel boundary. Analytical calculations show that the effect of clearance can be understood in terms of flow structures, such as the generic pusher and puller flows of active particles, indicating that our results should apply to a wide range of confined active particles.

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Swimming statistics of cargo-loaded single bacteria

Abstract: Burgeoning interest in the area of bacteria-powered micro robotic systems prompted us to study the dynamics of cargo transport by single bacteria. In this paper, we have studied the swimming...

Cited by 6 publications

References 33 publications

Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii^†

Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii^†

Photogyrotactic Concentration of a Population of Swimming Microalgae Across a Porous Layer

Fore-aft clearance controls how three-dimensional confinement affects micropropulsion

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Swimming statistics of cargo-loaded single bacteria

Abstract: Burgeoning interest in the area of bacteria-powered micro robotic systems prompted us to study the dynamics of cargo transport by single bacteria. In this paper, we have studied the swimming...

Cited by 6 publications

References 33 publications

Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii†

Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii†

Photogyrotactic Concentration of a Population of Swimming Microalgae Across a Porous Layer

Fore-aft clearance controls how three-dimensional confinement affects micropropulsion

Contact Info

Product

Resources

About

Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii^†

Bio-hybrid micro-swimmers propelled by flagella isolated from C. reinhardtii^†