SWINE BRUCELLOSIS CAUSED BY Brucella suis BIOVAR 2 IN CROATIA

Cvetnić, Željko; Duvnjak, Sanja; Zdelar-Tuk, Maja; Reil, Irena; Mikulić, Marina; Cvetnić, Marija; Špičić, Silvio

doi:10.26873/svr-360-2017

Cited by 2 publications

(3 citation statements)

References 10 publications

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“…Our study revealed the true seroprevalence of Brucella infection in the wild boar population of 9.06%, and shows the first published data regarding Brucella seropositivity in the wild boar population in Serbia, to date. The presence of Brucella in the wild boar population in Serbia was not unexpected since this microorganism has been confirmed in neighbouring countries, where B. suis biovar 2 was predominantly isolated [ 25 , 26 ]. Moreover, Cvetnic et al [ 14 ] confirmed the presence of B. suis biovar 3 in wild boars.…”

Section: Discussionmentioning

confidence: 97%

“…A global comprehensive literature review and meta-analysis of Brucella in pigs from 2000 to 2020 reported that the overall prevalence of brucellosis in wild boars between 2006 and 2010 was 22.3% [ 27 ], while after 2010, the prevalence gradually decreased after the World Organisation for Animal Health (WOAH) proposed control safety standards for animal production [ 28 ]. De facto , the Brucella seroprevalence in wild boars in Croatia varied through the years, ranging from 1.3% to 27.6% during different periods [ 13 , 14 , 25 ]. These data are in accordance with our obtained results, showing a Brucella seroprevalence of 9.06%.…”

Section: Discussionmentioning

confidence: 99%

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First Report of Brucella Seroprevalence in Wild Boar Population in Serbia

Sapundzic¹,

Žutić²,

Stević

et al. 2022

Veterinary Sciences

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Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no studies regarding brucellosis in wild boars in Serbia have been published. During the hunting season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive, indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of Brucella seropositive animals were detected in the eastern parts of the country and in one of the central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a risk for the infection of other animals.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

First Report of Brucella Seroprevalence in Wild Boar Population in Serbia

Sapundzic¹,

Žutić²,

Stević

et al. 2022

Veterinary Sciences

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“…is a genus currently consisting of 13 species (Ledwaba et al, 2019), in contrast to the 6 species known in 2003 (Osterman and Moryion, 2003). Brucellae are known to infect wild and domestic animals such as wild boars, cows, sheep, goats, dogs (Godfroid, 2002), but have also recently been cultured from marine animals, common voles, red foxes, baboons, human breast implants and fish (Al Dahouk et al, 2007;Cvetnic et al, 2017;Scholz et al, 2008aScholz et al, , 2008bScholz et al, , 2010Scholz et al, , 2016Whatmore et al, 2014). Brucella suis was the first pathogen to be used as a bioweapon in the 1950s.…”

Section: Introductionmentioning

confidence: 99%

Učinkovitost sljedeće generacije sekvenciranja u dijagnostici bakterijskih zoonoza

et al. 2021

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Brucella , an extremely diverse but yet genetically highly homogenous genus of bacteria, has been a puzzle for scientists for many decades. These bacteria remain a prominent public health issue, particularly in the Balkan region. Correctly identifying and understanding the pathogen is a vital step in the epidemiology and epizootiology of any bacteria. Identification can be challenging, especially in the case of zoonotic species. This study aimed to implement fourth-generation sequencing in the typing of 11 Brucella suis strains kept in our archive and to compare this method to the classical and non-sequencing based molecular methods used to date. Classical biotyping is highly subjective and gave inconclusive results for 3 strains. Molecular methods used were multiplex PCR and RFLP methods since no one method can identify both species and biovar which is vital in the case of Brucella suis infections. Species and biovars of all the strains were successfully confirmed and in concordance with biotyping results. Oxford Nanopore long-read sequencing was used on a MinION device for next-generation sequencing (NGS). Various algorithms were implemented for genome assembly and BioNumerics 8.0 software was used for MLST identification and analysis. MLST 21 was used for biovar identification and epidemiological comparison of tested strains. The assembled genomes were 3,2 Mb in size and assembled into two chromosomes. MLST 21 analysis placed our strains into species and biovar clusters in concordance with other molecular tests used. To the extent of our knowledge, this is the first documented use of long-read sequencing in Brucella suis identification in this region. We conclude that bacteriological biotyping is outdated and host-specific identification in this genus is incorrect and that molecular characterisation is always the safer, faster and more suitable option. MinION sequencing proved to be a strong, accessible solution for species determination. Future study is required to determine how detailed genome information it can give, considering the error rate.

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SWINE BRUCELLOSIS CAUSED BY Brucella suis BIOVAR 2 IN CROATIA

Cited by 2 publications

References 10 publications

First Report of Brucella Seroprevalence in Wild Boar Population in Serbia

First Report of Brucella Seroprevalence in Wild Boar Population in Serbia

Učinkovitost sljedeće generacije sekvenciranja u dijagnostici bakterijskih zoonoza

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