Swine manure vermicomposting via housefly larvae (Musca domestica): The dynamics of biochemical and microbial features

Zhang, Zhijian; Wang, Hang; Zhu, Jun; Suneethi, Sundar; Zheng, JianGuo

doi:10.1016/j.biortech.2012.05.048

Cited by 87 publications

(56 citation statements)

References 33 publications

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“…Beta-lactams were also used occasionally. The housefly larva vermicomposting processes consisted of several stages in series: (i) seed pupation and eclosion, (ii) housefly oviposition, (iii) larval inoculation in unprocessed manure and vermicomposting, (iv) separation of larval residues, and (v) seed sifting and breeding (9). The unprocessed manure, with a surface density of 11.0 kg m Ϫ2 , was evenly spread on the surface of cement block pools (7.2 ϫ 2.5 ϫ 0.2 m) in greenhouse-assisted larva vermireactors prior to larva inoculation (10).…”

Section: Methodsmentioning

confidence: 99%

“…These additions increased the vermicomposting layer from 5 cm to 8 to 10 cm in depth. This semicontinuous feeding mode was shown to be an efficient technique for larval bioconversion (7,9). The life cycle of vermicomposting with inoculated larvae is 5 (summer) to 10 (winter) days, depending on the ambient temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The mass reduction was primarily due to moisture removal and organic biodegradation by larval metabolism and growth. Detailed information about these larva vermireactors, housefly oviposition, and larva residue separation processes has been reported previously (7,9,10). Field sampling and physicochemical measurements.…”

Section: Methodsmentioning

confidence: 99%

“…Compared with the traditional composting practice without larvae, vermicomposting is much more beneficial. Within 5 to 7 days, a typical cycle of larva vermicomposting can reduce the mass and moisture content of manure by 70 to 80%, reduce odor and the abundance of fecal coliform bacteria, and provide a dehydrated compost fertilizer (a reduction rate of 106 Ϯ 17 kg m Ϫ3 day Ϫ1 ) ready for land applications (9); this process can also produce a fresh larva yield of 95 to 120 kg m Ϫ3 fresh raw manure (7). Recent studies have focused on composting techniques to retain the nutritional values of manure for soil fertilization but have not deter-mined whether such techniques can reduce antimicrobials or associated resistance genes.…”

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confidence: 99%

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Housefly Larva Vermicomposting Efficiently Attenuates Antibiotic Resistance Genes in Swine Manure, with Concomitant Bacterial Population Changes

Wang

Gilbert

et al. 2015

Appl Environ Microbiol

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h Manure from swine treated with antimicrobials as feed additives is a major source for the expansion of the antibiotic resistance gene (ARG) reservoir in the environment. Vermicomposting via housefly larvae (Musca domestica) can be efficiently used to treat manure and regenerate biofertilizer, but few studies have investigated its effect on ARG attenuation. Here, we tracked the abundances of 9 ARGs and the composition and structure of the bacterial communities in manure samples across 6 days of fullscale manure vermicomposting. On day 6, the abundances of genes encoding tetracycline resistance [tet(M), tet(O), tet(Q), and tet(W)] were reduced (P < 0.05), while those of genes encoding sulfonamide resistance (sul1 and sul2) were increased (P < 0.05) when normalized to 16S rRNA. The abundances of tetracycline resistance genes were correlated (P < 0.05) with the changing concentrations of tetracyclines in the manure. The overall diversity and richness of the bacteria significantly decreased during vermicomposting, accompanied by a 100 times increase in the relative abundance of Flavobacteriaceae spp. Variations in the abundances of ARGs were correlated with the changing microbial community structure and the relative abundances of the family Ruminococcaceae, class Bacilli, or phylum Proteobacteria. Vermicomposting, as a waste management practice, can reduce the overall abundance of ARGs. More research is warranted to assess the use of this waste management practice as a measure to attenuate the dissemination of antimicrobial residues and ARGs from livestock production before vermicompost can be safely used as biofertilizer in agroecosystems.A ntimicrobials have been widely used at therapeutic levels for microbial infection control and at subtherapeutic levels for growth promotion as veterinary feed additives, administered in water or parenterally in animal husbandry (1). Antimicrobials can select for antibiotic resistance genes (ARGs) in host-associated bacteria from concentrated animal feeding operations and in the environment where antimicrobial-containing feces are excreted or applied to fields (2). Through horizontal gene transfer mechanisms triggered by mobile genetic elements, such as conjugative plasmids, integrons, and transposons (3, 4), different microbes can share their genetic information, potentially causing the transfer and dissemination of ARGs from feces-associated bacteria to indigenous environmental microbes. For instance, an intI1 gene encoding a site-specific integrase responsible for integration is closely related to the transfer of sulfonamide resistance genes among bacteria (5). Specifically, swine manure increases the resistant gene reservoir in the environment, as most applied veterinary antimicrobials are poorly absorbed by livestock (6). These antimicrobials are largely excreted in feces and then dispersed to soil when the manure is used as fertilizer. Unmanaged waste disposal is commonly regarded as one of the major sources for the environmental expansion of ARGs (3); therefore, it is ext...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Housefly Larva Vermicomposting Efficiently Attenuates Antibiotic Resistance Genes in Swine Manure, with Concomitant Bacterial Population Changes

Wang

Gilbert

et al. 2015

Appl Environ Microbiol

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“…Čičkováet al (2012 demonstrated that Muscadomestica, the most frequently found species reduce 1 kg of pig dejectionto 0.18 kg. Zhang et al (2012), Zhu et al (2012), Zhu et al (2015) and Wang et al (2016) reported also an important loss of carbon and nitrogen in the swine manure after housefly (Muscadomestica) larvae production. Such potential of housefly can be harnessed in rural areas under the tropical conditions, especially in Sub-Saharan Africa.…”

Section: Introductionmentioning

confidence: 99%

Physical and Chemical Properties of Animals ’ Organic Residues Decomposed by Musca domestica and Calliphora vomitoria Larvae

A.Y¹

2017

JAES

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Our study aims to assess physical and chemical properties of organic residues decomposed by fly larvae to be promoted as organic manure. Three stages of four days duration each of biodegradation of 12 various animal substrates (pig, poultry, guinea fowl, sheep, cow, mixture of pig and sheep substrates, mixture of pig and cow substrates, mixture of poultry and sheep substrates, mixture of poultry and cow substrates, mixture of guinea fowl and sheep substrates, mixture of guinea fowl and cow substrates) using Muscadomestica and Calliphoravomitoria larvae were performed. Temperature, pH, weight, organic carbon, total nitrogen, nitrite and ammonia contents during decomposition process were data collected. The highest temperatures were recorded respectively in the pig and poultry substrates. The pH changed from neutral to alkaline. Organic carbon content reduced during the biodegradation process and was positively correlated with reduction of substrate's weight. Significant decrease of ammonia content was recorded respectively during the first, second and third stages of biodegradation process in the poultry substrate (80, 46 and 61%), guinea fowl (77, 26 and 27%) and pig substrate (76, 66, and 40%). Furthermore, nitrite content was low ranging from 0.0033 to 0.075 gkg -1 . The stability and maturity of the final product were high with pig substrates, mixture of poultry and sheep substrates, mixture of poultry and cow substrates, mixture of pig and sheep substrates. It is suggested to give priority to the later substrates for soil fertility management.

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Strategic Intervention for Climate-Smart Agriculture

Sakha,

Gweyi-Onyango

2024

Technological Approaches for Climate Smart Agriculture

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Swine manure vermicomposting via housefly larvae (Musca domestica): The dynamics of biochemical and microbial features

Cited by 87 publications

References 33 publications

Housefly Larva Vermicomposting Efficiently Attenuates Antibiotic Resistance Genes in Swine Manure, with Concomitant Bacterial Population Changes

Housefly Larva Vermicomposting Efficiently Attenuates Antibiotic Resistance Genes in Swine Manure, with Concomitant Bacterial Population Changes

Physical and Chemical Properties of Animals ’ Organic Residues Decomposed by Musca domestica and Calliphora vomitoria Larvae

Strategic Intervention for Climate-Smart Agriculture

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