Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning

Abstract

Swiprosin-1 (EFhD2) is a molecule that triggers structural adaptation of isolated adult rat cardiomyocytes to cell culture conditions by initiating a process known as cell spreading. This process mimics central aspects of cardiac remodeling, as it occurs subsequent to myocardial infarction. However, expression of swiprosin-1 in cardiac tissue and its regulation in vivo has not yet been addressed. The expression of swiprosin-1 was analyzed in mice, rat, and pig hearts undergoing myocardial infarction or ischemia/reperfusion with or without cardiac protection by ischemic pre- and postconditioning. In mouse hearts, swiprosin-1 protein expression was increased after 4 and 7 days in myocardial infarct areas specifically in cardiomyocytes as verified by immunoblotting and histology. In rat hearts, swiprosin-1 mRNA expression was induced within 7 days after ischemia/reperfusion but this induction was abrogated by conditioning. As in cultured cardiomyocytes, the expression of swiprosin-1 was associated with a coinduction of arrestin-2, suggesting a common mechanism of regulation. Rno-miR-32-3p and rno-miR-34c-3p were associated with the regulation pattern of both molecules. Moreover, induction of swiprosin-1 and ssc-miR-34c was also confirmed in the infarct zone of pigs. In summary, our data show that up-regulation of swiprosin-1 appears in the postischemic heart during cardiac remodeling and repair in different species.

Scutellarin Protects against Myocardial Ischemia-Reperfusion Injury by Enhancing Aerobic Glycolysis via miR-34c-5p/ALDOA axis

“…Tu Y, et al found miR-34c-5p played a crucial role in cerebral IR injury via in ammatory and apoptotic signaling pathways [40,41]. According to reports, miR-34c-5p participates in cardiac remodeling and repair during post-ischemic heart injury [42]. Our research supplemented the above results.…”

Scutellarin Protects against Myocardial Ischemia–reperfusion Injury by Enhancing Aerobic Glycolysis through miR-34c-5p/ALDOA Axis

“…[ 38 39 ] According to reports, miR-34c-5p participates in cardiac remodeling and repair during postischemic heart injury. [ 40 ] Our research supplemented the above results. In addition, we observed that HR condition increased the level of miR-138-5p and Scu treatment enhanced this effect, which indicated that Scu might aggravated HR injury trough upregulate miR-138-5p.…”

Structural and biochemical insights into Zn2+-bound EF-hand proteins, EFhd1 and EFhd2

“…In addition, EFhd2 may play an important role in several neurodegenerative diseases, as it is associated with tau in Alzheimer's disease and other neurological disorders (Ferrer-Acosta et al, 2013;Vega, 2016). Recently, it was found that EFhd2 is up-regulated in the cardiomyocytes during cardiac remodeling and repair (Giricz et al, 2020). Although the functions and cellular localization of EFhd1 and EFhd2 differ, the two proteins exhibit a high degree of sequence identity (65%) (Mun et al, 2021;Dutting et al, 2011;Park et al, 2016).…”