Switch on or switch off: An optical DNA sensor based on poly(p-phenylenevinylene) grafted magnetic beads

Srinivas, Anupama R. Gulur; Peng, Hui; Barker, David; Travaš-Sejdić, Jadranka

doi:10.1016/j.bios.2012.03.022

Cited by 23 publications

(10 citation statements)

References 39 publications

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“…The ease of synthesis of ICPs and theirs unique properties encourages researchers to employ this material in the fabrication of biosensors. Some of the ICPs groups that are frequently used in biosensors application are polyacetylene [81], polythiophene [82], poly(p-phenylenevinylene) [83], poly(3,4-ethylenedioxythiophene) [84], polypyrrole [85], and polyaniline [86] where their structures are shown in Fig. 9.…”

Section: Electrochemical Sensors Using Aunp Nanocomposites With Organmentioning

confidence: 99%

Gold nanoparticle based optical and electrochemical sensing of dopamine

et al. 2015

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This review (with 110 refs.) gives an overview on the progress that has been made in the past few years on the use of gold nanoparticles (AuNPs) for use in sensors and analytical tools for the determination of dopamine (DA). Both AuNPs and their composites with other organic and inorganic materials including noble metals are treated. Following an overview on the clinical significance of DA, we discuss the various analytical methods that are (a) electrochemiluminescence (ECL); (b) surface enhanced Raman scattering (SERS); (c) colorimetric probing and visual detection; and (d) the large class of electrochemical sensors. Subsections cover sensors based on plain AuNPs, bimetallic NPs, AuNP-metal@metal oxide nanocomposites, AuNP nanocomposites with organic polymers, AuNP nanocomposites with carbon nanotubes or with graphene, and finally sensors based on ternary materials containing AuNPs. The review ends with a conclusion on current challenges of sensors for DA and an outlook on future trends.

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Section: Electrochemical Sensors Using Aunp Nanocomposites With Organmentioning

confidence: 99%

Gold nanoparticle based optical and electrochemical sensing of dopamine

et al. 2015

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“…Nonetheless, fluorescent polymers, especially CPs, have found application in a number of fluorescence-based studies, including FRET [130]. Fluorescent CPs, cationic conjugated polymers (CCPs), and conjugated polyelectrolytes (CPEs) are particularly popular for developing FRET-based DNA biosensors [132], with applications ranging from detection of DNA [133,134], DNA hybridization [135], DNA methylation [136], and SNPs [137]. They have also been incorporated into sensing schemes for proteins [138] and Hg ions [89,139].…”

Section: Dendrimers and Polymer Macromoleculesmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

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The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

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“…Functional magnetic beads (MBs) have preferentially been used in various biological and biomedical fields such as bioseparation, biosensors, single nucleotide polymorphisms genotyping, magnetic resonance imaging and drug delivery [13][14][15][16][17] . We and other groups have developed a method by conjuncting MBs based ECL with PCR or RT-PCR [18][19][20] .…”

Section: Introductionmentioning

confidence: 99%

Development of an in situ magnetic beads based RT-PCR method for electrochemiluminescent detection of rotavirus

Zhan

Zhou

2012

SPIE Proceedings

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Rotaviruses are double-stranded RNA viruses belonging to the family of enteric pathogens. It is a major cause of diarrhoeal disease in infants and young children worldwide. Consequently, rapid and accurate detection of rotaviruses is of great importance in controlling and preventing food-and waterborne diseases and outbreaks. Reverse transcription-polymerase chain reaction (RT-PCR) is a reliable method that possesses high specificity and sensitivity. It has been widely used to detection of viruses. Electrochemiluminescence (ECL) can be considered as an important and powerful tool in analytical and clinical application with high sensitivity, excellent specificity, and low cost. Here we have developed a method for the detection of rotavirus by combining in situ magnetic beads (MBs) based RT-PCR with ECL.RT of rotavirus RNA was carried out in a traditional way and the resulting cDNA was directly amplified on MBs.Forward primers were covalently bounded to MBs and reverse primers were labeled with tris-(2, 2'-bipyridyl) ruthenium (TBR). During the PCR cycling, the TBR labeled products were directly loaded and enriched on the surface of MBs.Then the MBs-TBR complexes could be analyzed by a magnetic ECL platform without any post-modification or post-incubation，which avoid some laborious manual operations and achieve rapid yet sensitive detection. In this study, rotavirus from fecal specimens was successfully detected within 2 h, and the limit of detection was estimated to be 10 4 copies/μL. This novel in situ MBs based RT-PCR with ECL detection method can be used for pathogen detection in food safety field and clinical diagnosis.

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Switch on or switch off: An optical DNA sensor based on poly(p-phenylenevinylene) grafted magnetic beads

Cited by 23 publications

References 39 publications

Gold nanoparticle based optical and electrochemical sensing of dopamine

Gold nanoparticle based optical and electrochemical sensing of dopamine

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Development of an in situ magnetic beads based RT-PCR method for electrochemiluminescent detection of rotavirus

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