Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior

Rolli, Claudio G.; Nakayama, Hidekazu; Yamaguchi, Kazuo; Spatz, Joachim P.; Kemkemer, Ralf; Nakanishi, Jun

doi:10.1016/j.biomaterials.2011.12.012

Cited by 132 publications

(142 citation statements)

References 34 publications

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“…49 After thoroughly washing the substrates, a passivated glass surface was obtained, on which cells were not able to adhere. 34 By introducing a photomask in the field diaphragm of an inverted microscope, equipped with an HBO mercury arc lamp (Olympus) and focusing UV light through an objective (UPlanSApo 10Â/0.4, Olympus) with a wavelength of 365 nm on the photoactive substrate and exposing it with a dose of 10 J/cm 2 , the PEG12k-NH is cleaved off. Thereby, the surface at the irradiated regions became cell-adhesive.…”

Section: Methodsmentioning

confidence: 99%

“…This dissimilar cell behavior can be easily ascribed to the massive shift in the expression of genes involved in cell-matrix interaction (collagens, laminins, integrins) observed in cells in which YAP/ TAZ were silenced (siYAP, Figure 3b). Given that YAP/ TAZ nuclear activity seems to be important for the adhesion of hCMPCs onto stiff matrices, photoactivated glass surfaces (E = ¥) 34 were prepared in which cells are confined in 900 μm 2 adhesive areas, without the possibility to spread (Figure 3c and Supplementary Figure 4c). As expected, in this condition, YAP/TAZ staining was mainly detected in cell cytoplasm, despite substrate stiffness being virtually infinite.…”

Section: Articlementioning

confidence: 99%

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Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

et al. 2014

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T he Hippo pathway has been recently identified as a crucial axis in the regulation of organ size and shape during organogenesis and cancer. The paralog Yes-associated protein (YAP) and WW domain-containing transcription regulator protein 1 (WWTR1 or TAZ) are the downstream effectors of the Hippo pathway. These proteins have also been identified as mammalian proto-oncogenes. 1 Moreover, they perform as transcriptional coactivators in the nucleus, mainly in combination with transcription factors belonging to TEAD family. 2 In vitro, YAP/TAZ activity has been associated with mesenchymal stem cell (MSC) fate decision through the interaction with key determinants of osteogenic (Runx2) or adipogenic (PPARγ) differentiation. 3,4 YAP/TAZ axis has also emerged as a central regulator of human embryonic stem cell self-renewal through the control of SMAD complex shuttling to the nucleus, with TAZ knock-down resulting in the loss of cell pluripotency. 5 The same cofactors control intestinal 6 and neural progenitor cell number and differentiation 7 by targeting We identify a novel activity of YAP and TAZ in the regulation of tubulogenesis in 3D environments and highlight a role for YAP/TAZ in cardiac progenitor proliferation and differentiation. Furthermore, we show that YAP/TAZ expression is triggered in the heart cells located at the infarct border zone. Our results suggest a fundamental role for the YAP/TAZ axis in the response of resident progenitor cells to the modifications in microenvironment nanostructure and mechanics, thereby contributing to the maintenance of myocardial homeostasis in the adult heart. These proteins are indicated as potential targets to control cardiac progenitor cell fate by materials design.

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Section: Methodsmentioning

confidence: 99%

Section: Articlementioning

confidence: 99%

Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

et al. 2014

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“…In order to create such dynamic adhesion environments, unidirectional, and irreversible switching of integrin-mediated adhesion has been achieved by light-triggered activation of caged molecules and by electrochemical switching. [5][6][7] Studies using this approach revealed that the time point of cell exposure to adhesion ligands and their density signifi cantly infl uences cell differentiation [ 8 ] and guides vascularization in vivo. [ 9 ] This shows that cells keep track of their adhesion history and that dynamic changes in adhesion signals are imperative in controlling cell functions.…”

Section: Doi: 101002/adma201504394mentioning

confidence: 99%

Rapid Reversible Photoswitching of Integrin‐Mediated Adhesion at the Single‐Cell Level

et al. 2015

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“…9,10 Cells are seeded on the substrate in excess and, aer a short incubation time, the pattern is rinsed in order to remove non-adherent cells. [11][12][13][14] The difficulty resides in adjusting the initial cell concentration so that a single cell is le on each adhesive spot. The initial distribution of trapped cells per adhesive island is highly dependent on the seeding density.…”

Section: Introductionmentioning

confidence: 99%

Cellular self-organization on micro-structured surfaces

2014

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Micro-patterned surfaces are frequently used in high-throughput single-cell studies, as they allow one to image isolated cells in defined geometries. Commonly, cells are seeded in excess onto the entire chip, and non-adherent cells are removed from the unpatterned sectors by rinsing. Here, we report on the phenomenon of cellular self-organization, which allows for autonomous positioning of cells on micropatterned surfaces over time. We prepared substrates with a regular lattice of protein-coated adhesion sites surrounded by PLL-g-PEG passivated areas, and studied the time course of cell ordering. After seeding, cells randomly migrate over the passivated surface until they find and permanently attach to adhesion sites. Efficient cellular self-organization was observed for three commonly used cell lines (HuH7, A549, and MDA-MB-436), with occupancy levels typically reaching 40-60% after 3-5 h. The time required for sorting was found to increase with increasing distance between adhesion sites, and is well described by the time-to-capture in a random-search model. Our approach thus paves the way for automated filling of cell arrays, enabling high-throughput single-cell analysis of cell samples without losses.

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Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior

Cited by 132 publications

References 34 publications

Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

Hippo Pathway Effectors Control Cardiac Progenitor Cell Fate by Acting as Dynamic Sensors of Substrate Mechanics and Nanostructure

Rapid Reversible Photoswitching of Integrin‐Mediated Adhesion at the Single‐Cell Level

Cellular self-organization on micro-structured surfaces

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