Switchable Fluorescent Imaging of Intracellular Telomerase Activity Using Telomerase-Responsive Mesoporous Silica Nanoparticle

Qian, Ruo‐Can; Ding, Lin; Ju, Huangxian

doi:10.1021/ja406532e

Cited by 224 publications

(162 citation statements)

References 33 publications

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“…Hence, NFs are not favorable for situations in which quick drug release is necessary. In the fields of intracellular biosensing and analyte detection, NFs do not have advantages, in contrast to previously reported biosensing platforms such as graphene oxide nanosheets 52 , nanoparticles 53 , DNA dendrimers 22 and DNA tetrahedra 20 . This is because large quantities of DNA probes are packaged inside NFs, which means that they cannot function well toward target molecules.…”

Section: Introductionmentioning

confidence: 60%

Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers

Lv¹,

Hu²,

Zhu³

et al. 2015

Nat Protoc

158

123

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We describe a comprehensive protocol for the preparation of multifunctional DNA nanostructures termed nanoflowers (NFs), which are self-assembled from long DNA building blocks generated via rolling-circle replication (RCR) of a designed template. NF assembly is driven by liquid crystallization and dense packaging of building blocks, which eliminates the need for conventional Watson-Crick base pairing. As a result of dense DNA packaging, NFs are resistant to nuclease degradation, denaturation or dissociation at extremely low concentrations. By manually changing the template sequence, many different functional moieties including aptamers, bioimaging agents and drug-loading sites could be easily integrated into NF particles, making NFs ideal candidates for a variety of applications in biomedicine. In this protocol, the preparation of multifunctional DNA NFs with highly tunable sizes is described for applications in cell targeting, intracellular imaging and drug delivery. Preparation and characterization of functional DNA NFs takes ~5 d; the following biomedical applications take ~10 d.

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Section: Introductionmentioning

confidence: 60%

Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers

Lv¹,

Hu²,

Zhu³

et al. 2015

Nat Protoc

158

123

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show abstract

“…For this purpose, Ju and co-workers developed a nanodevice able to the measure the presence of telomerase activity with a DNA-MSNs hybrid. 200 In their design the authors employed the telomerase substrate sequence 5'-[(CCC TAA) n AAT CCG TCG AGC AGA GTT]-3' to coat electrostatically their NH 2 -modified MSNs. The system was completed with black hole quencher grafted into the internal pore walls; this produced fluorescence quenching unless telomerase-induced pore release of the loaded fluorescein occurred.…”

Section: Sensors For the Detection Of Enzymatic Activitymentioning

confidence: 99%

Recent applications of the combination of mesoporous silica nanoparticles with nucleic acids: development of bioresponsive devices, carriers and sensors

Castillo¹,

Baeza²

2017

Biomater. Sci.

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The discovery and control of the biological roles mediated by nucleic acids have turned them into a powerful tool for the development of advanced biotechnological materials. Much is the importance of those gene-keeping biomacromolecules that even nanomaterials have succumbed to the claimed benefits of DNA and RNA. Currently, there could be found in the literature a practically intractable number of examples which report the use in combination of nanoparticles with nucleic acids, which demands boundedness. Following this premise, this revision will only cover the most recent and powerful strategies developed to exploit the possibilities of nucleic acids as biotechnological materials when in combination with mesoporous silica nanoparticles. The extensive research done on nucleic acids has significantly incremented the technological possibilities for those biomacromolecules, which could be employed in many different applications; where substrate or sequence recognition or modulation of biological pathways due to its coding role in living cells are the most promising. In the present revision, the chosen counterpart, mesoporous silica nanoparticles, also with unique properties, became a reference material for drug delivery and biomedical applications due to their high biocompatibility and porous structure suitable for hosting and delivering small molecules. Although most of revisions deal with significant advances in the use of nucleic acid and mesoporous silica nanoparticles in biotechnological applications, a rationale classification of those new generation hybrid materials is still uncovered. Along this review there will be covered promising strategies for living cell and biological sensors, DNA-based molecular gates with targeting, transfection or silencing properties which could provide a significant advance of current nanomedicine.

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“…88 These authors anchored a fluorescent quencher to the inner pore surfaces in MSNs and further loaded pores with fluorescein. Then the external surface of MSNs was functionalised with APTES and capped with a DNA sequence via electrostatic interactions, capable of activating telomerase (see Figure 8).…”

Section: Telomerasementioning

confidence: 99%

Mesoporous silica materials for controlled delivery based on enzymes

et al. 2017

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Switchable Fluorescent Imaging of Intracellular Telomerase Activity Using Telomerase-Responsive Mesoporous Silica Nanoparticle

Cited by 224 publications

References 33 publications

Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers

Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers

Recent applications of the combination of mesoporous silica nanoparticles with nucleic acids: development of bioresponsive devices, carriers and sensors

Mesoporous silica materials for controlled delivery based on enzymes

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