Switchable stimulated Raman scattering microscopy with photochromic vibrational probes

Ao, Jianpeng; Fang, Xiaofeng; Miao, Xianchong; Ling, Jiwei; Kang, Hyung Seok; Park, Sungnam; Wu, Changfeng; Ji, Minbiao

doi:10.1038/s41467-021-23407-2

Cited by 56 publications

(61 citation statements)

References 45 publications

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“…Using SRS imaging, they demonstrated the photoswitching ability in live HeLa cells and also visualized mitochondria diffusion using pulse-chase experiments. 130 Similar to switchable nitrile probes, photoswitchable alkyne probes also have potential application toward super-resolution SRS imaging in the cell silent region. Raman imaging is already used in clinical diagnostics and is being translated from bench to bedside.…”

Section: Discussionmentioning

confidence: 99%

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

2021

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Section: Discussionmentioning

confidence: 99%

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

2021

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“…Combining local vibrational reporters with dyes and fluorescence molecules can simultaneously take advantage of fluorescence and Raman scattering and enhance vibration sensitivity. Recently, Ji et al [201] reported a Raman probe based on alkynetagged diarylethene derivatives and realized photoswitchable SRS imaging. Under UV irradiation, the photo isomerization of these conjugated derivatives produced a frequency red shift in the SRS spectra of the narrow Raman peak of the alkyne group, making the absorption wavelength closer to the SRS pump wavelength.…”

Section: Bioimagingmentioning

confidence: 99%

“…Recently, Ji et al. [ 201 ] reported a Raman probe based on alkyne‐tagged diarylethene derivatives and realized photoswitchable SRS imaging. Under UV irradiation, the photoisomerization of these conjugated derivatives produced a frequency red shift in the SRS spectra of the narrow Raman peak of the alkyne group, making the absorption wavelength closer to the SRS pump wavelength.…”

Section: Changes In the Physical And Chemical Properties Of Daementioning

confidence: 99%

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Future‐Oriented Advanced Diarylethene Photoswitches: From Molecular Design to Spontaneous Assembly Systems

et al. 2022

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cycloreversion of DAE under light irradiation produces many exciting functional effects, such as changes in photophysical and photochemical properties, [12] changes in the shape or mechanical properties of the solid, [13] electrical conductivity, [7b,14] and the generation of reactive oxygen species (ROS). [15] This type of photochromic compound can be considered a stilbene derivative. At this time, the most widely known and systematically studied DAE derivative structure contains two aryl groups and a benzene ring (usually two identical or different thiophene units with low aromatic stabilization energy [16] ) and a five-membered ring of perfluorocyclopentene and cyclopentene units [17] where the ethene bridge is located, which significantly improve photochromism. [18] In essence, the DAE photoisomerization process is a reversible electrocyclic reaction of the central 6π-electron systems. [2] Under light irradiation, DAE can rapidly and reversibly transform between its open and closed forms, and that transformation is accompanied by a color change caused by a change in the π-conjugation distribution. [2] The important thing is that the colorless opened-and colored closed-ring isomers are both thermally stable; that is, cyclization and cycloreversion do not proceed spontaneously in the dark; they need to be driven by external light irradiation at specific wavelengths. [2,19] Compared with classic lightresponsive molecules such as azobenzene, [20] the light intensity required to drive the isomerization of DAE is relatively low. Moreover, even after multiple cycles, the photochromic performance does not show significant fatigue in either the solution or solid state. [2,18b,21] DAE research to this point can be roughly divided into three stages in chronological order: early exploration of DAE's molecular structure and theory, nearly two decades of purpose-oriented molecular structure modification research, and about ten years of research into practical applications of DAE in assembly systems. Research before about 2000 focused mainly on developing more abundant DAE-derivative structures and advancing the systematic understanding of DAE's physical and chemical properties and switching performance. To regulate its switching performance and give it new functionality, many strategies for modifying the ethene bridge and aryl groups of DAE have been proposed. [22] In the first two research stages, the mechanisms and structural modification of DAE isomerization were thoroughly and systematically explored, and countless important Diarylethene (DAE) photoswitch is a new and promising family of photochromic molecules and has shown superior performance as a smart trigger in stimulus-responsive materials. During the past few decades, the DAE family has achieved a leap from simple molecules to functional molecules and developed toward validity as a universal switching building block. In recent years, the introduction of DAE into an assembly system has been an attractive strategy that enables the photochromic behavior of the b...

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“…Such methods can achieve a few nanometers or sub-nanometer resolution (7)(8)(9). Recently, several super-resolution SRS techniques have been developed (10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Nonetheless, it is still challenging to achieve super-resolved Raman imaging without manipulating the samples, and to preserve the temporal resolution without any labeling or additional physical or chemical treatment.…”

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confidence: 99%

Super-resolution stimulated Raman Scattering microscopy with A-PoD

Jang

Fung

et al. 2022

Preprint

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Unlike traditionally-mapped Raman imaging, stimulated Raman scattering (SRS) imaging achieved the capability of imaging metabolic dynamics and a greatly improved signal-noise-ratio. However, its spatial resolution is still limited by the numerical aperture or scattering cross section. To achieve super-resolved SRS imaging, we developed a new deconvolution algorithm Adam optimization-based Pointillism Deconvolution (A-PoD) for SRS imaging, and demonstrated a spatial resolution of 52 nm on polystyrene beads. By changing the genetic algorithm to A-PoD, the image deconvolution process was shortened by more than 3 orders of magnitude, from a few hours to a few seconds. By applying A-PoD to spatially correlated multi-photon fluorescence (MPF) imaging and deuterium oxide (D2O)-probed SRS (DO-SRS) imaging data from diverse samples, we compared nanoscopic distributions of proteins and lipids in cells and subcellular organelles. We successfully differentiated newly synthesized lipids in lipid droplets using A-PoD coupled with DO-SRS. The A-PoD-enhanced DO-SRS imaging method was also applied to reveal the metabolic change in brain samples from Drosophila on different diets. This new approach allows us to quantitatively measure the nanoscopic co-localization of biomolecules and metabolic dynamics in organelles. We expect that the A-PoD algorithm will have a wide range of applications, from nano-scale measurements of biomolecules to processing astronomical images.

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Switchable stimulated Raman scattering microscopy with photochromic vibrational probes

Cited by 56 publications

References 45 publications

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

Future‐Oriented Advanced Diarylethene Photoswitches: From Molecular Design to Spontaneous Assembly Systems

Super-resolution stimulated Raman Scattering microscopy with A-PoD

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