Switchavidin: Reversible Biotin–Avidin–Biotin Bridges with High Affinity and Specificity

Taskinen, Barbara; Zauner, Dominik; Lehtonen, Soili I.; Koskinen, Masi; Thomson, Chloe; Kähkönen, Niklas; Kukkurainen, Sampo; Määttä, Juha; Ihalainen, Teemu O.; Kulomaa, Markku S.; Gruber, Hermann J.; Hytönen, Vesa P.

doi:10.1021/bc500462w

Cited by 32 publications

(38 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other recombinant or naturally occurring avidin include Tamavidin 2 ( Pleurotus cornucopiae )[40], Shwanavidin ( Shewanella denitrificans )[41], Switchavidin (chicken avidin mutant)[42], Zebavidin (Zebrafish)[43]. …”

Section: Biochemical Insights Of Avidin Biotin and Analoguesmentioning

confidence: 99%

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Jain

Cheng

2017

Journal of Controlled Release

227

286

View full text Add to dashboard Cite

Avidin-biotin interaction is one of the strongest non-covalent interactions in the nature. Avidin and its analogues have therefore been extensively utilized as probes and affinity matrices for a wide variety of applications in biochemical assays, diagnosis, affinity purification, and drug delivery. Recently, there has been a growing interest in exploring this non-covalent interaction in nanoscale drug delivery systems for pharmaceutical agents, including small molecules, proteins, vaccines, monoclonal antibodies, and nucleic acids. Particularly, the ease of fabrication without losing the chemical and biological properties of the coupled moieties makes the avidin-biotin system a versatile platform for nanotechnology. In addition, avidin-based nanoparticles have been investigated as diagnosis systems for various tumors and surface antigens. In this review, we will highlight the various fabrication principles and biomedical applications of avidin-based nanoparticles in drug delivery and diagnosis. The structures and biochemical properties of avidin, biotin and their respective analogues will also be discussed.

show abstract

Section: Biochemical Insights Of Avidin Biotin and Analoguesmentioning

confidence: 99%

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Jain

Cheng

2017

Journal of Controlled Release

227

286

View full text Add to dashboard Cite

show abstract

“…Several elution methods have been developed; using detergents, 7 extremely low pH 8 or solvents 9 and alternative strategies have been employed including weakened affinity of streptavidin/avidin to biotin through chemical 10 or genetic 11 means. However, even these modifications only lead to partial recovery of biotinylated proteins from streptavidin, 12 and nonbiotinylated peptides always vastly outnumber biotinylated peptides. 2 The need for good elution strategies has been circumvented by the use of on-bead digestion, which has become a convenient and widely used method in proximity-dependent biotinylation experiments.…”

Section: Introductionmentioning

confidence: 99%

BioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation

et al. 2017

View full text Add to dashboard Cite

Biotin-based labeling strategies are widely employed to study protein-protein interactions, subcellular proteomes and post-translational modifications, as well as, used in drug discovery. While the high affinity of streptavidin for biotin greatly facilitates the capture of biotinylated proteins, it still presents a challenge, as currently employed, for the recovery of biotinylated peptides. Here we describe a strategy designated Biotinylation Site Identification Technology (BioSITe) for the capture of biotinylated peptides for LC−MS/MS analyses. We demonstrate the utility of BioSITe when applied to proximity-dependent labeling methods, APEX and BioID, as well as biotin-based click chemistry strategies for identifying O-GlcNAc-modified sites. We demonstrate the use of isotopically labeled biotin for quantitative BioSITe experiments that simplify differential interactome analysis and obviate the need for metabolic labeling strategies such as SILAC. Our data also highlight the potential value of site-specific biotinylation in providing spatial and topological information about proteins and protein complexes. Overall, we anticipate that BioSITe will replace the conventional methods in studies where detection of biotinylation sites is important.

show abstract

“…[38][39][40] Recently, avidin enabling release from biotinylated materials has been described, offering a potential way to develop regenerable biosensors. 27,41,42 In conclusion, biotinylated BSA surface functionalized PLA-PEG blend fibers were successfully prepared by electrospinning technique. PLA fibers showed porous, beadless and interconnected structure.…”

Section: Discussionmentioning

confidence: 90%

Mixture of PLA–PEG and biotinylated albumin enables immobilization of avidins on electrospun fibers

Kumar¹,

Rahikainen

Unruh

et al. 2016

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

The application of nanotechnology in biomedical field has enormous potential in basic and applied research. Micro or nanofibers produced by electrospinning technique offer excellent properties because of large specific surface area, high porosity, and ability to incorporate functional additives. Here we embedded biotinylated bovine serum albumin into polylactic acid (PLA)-polyethylene glycol (PEG) fibers, which enabled specific immobilization of fluorescently labelled avidin. An alkaline phosphatase enzyme was immobilized via biotin-streptavidin interaction on the hybrid nanofibers, demonstrating the suitability of the material for biosensing applications. These functional nanofibers provide a promising platform for development of biosensors and other biofunctional materials utilizing avidin-biotin as a generic and robust immobilization method. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 356-362, 2017.

show abstract

Switchavidin: Reversible Biotin–Avidin–Biotin Bridges with High Affinity and Specificity

Cited by 32 publications

References 48 publications

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

BioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation

Mixture of PLA–PEG and biotinylated albumin enables immobilization of avidins on electrospun fibers

Contact Info

Product

Resources

About