Switching specific biomolecular interactions on surfaces under complex biological conditions

Lashkor, Minhaj; Rawson, Frankie J.; Preece, Jon A.; Mendes, Paula M.

doi:10.1039/c4an01225a

Cited by 21 publications

(10 citation statements)

References 46 publications

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“…Three peaks were deconvoluted in the C 1s XPS spectrum. Peaks with the centres at 285.0, 286.2 and 289.2 eV can be attributed to C–C/C–H bonds of adamantyl-moiety and the HeA side chain, then to the C–N bond of the amino groups 43 and finally to the C–O bond of the carboxylic acid group of HeA, respectively 45 . The latter could support an assumption about the deprotonated nature of the carboxylic group because of exhibiting one signal due to the delocalized character of the negative charge 46 .…”

Section: Resultsmentioning

confidence: 99%

Synthesis conditions influencing formation of MAPbBr3 perovskite nanoparticles prepared by the ligand-assisted precipitation method

Procházková

Scharber

Yumusak

et al. 2020

Sci Rep

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This work reports on an optimized procedure to synthesize methylammonium bromide perovskite nanoparticles. The ligand-assisted precipitation synthetic pathway for preparing nanoparticles is a cost-effective and promising method due to its ease of scalability, affordable equipment requirements and convenient operational temperatures. Nevertheless, there are several parameters that influence the resulting optical properties of the final nanomaterials. Here, the influence of the choice of solvent system, capping agents, temperature during precipitation and ratios of precursor chemicals is described, among other factors. Moreover, the colloidal stability and stability of the precursor solution is studied. All of the above-mentioned parameters were observed to strongly affect the resulting optical properties of the colloidal solutions. Various solvents, dispersion media, and selection of capping agents affected the formation of the perovskite structure, and thus qualitative and quantitative optimization of the synthetic procedure conditions resulted in nanoparticles of different dimensions and optical properties. The emission maxima of the nanoparticles were in the 508–519 nm range due to quantum confinement, as confirmed by transmission electron microscopy. This detailed study allows the selection of the best optimal conditions when using the ligand-assisted precipitation method as a powerful tool to fine-tune nanostructured perovskite features targeted for specific applications.

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Section: Resultsmentioning

confidence: 99%

Synthesis conditions influencing formation of MAPbBr3 perovskite nanoparticles prepared by the ligand-assisted precipitation method

Procházková

Scharber

Yumusak

et al. 2020

Sci Rep

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“…Much attention is nowadays being paid to the design of stimuli-responsive surfaces that facilitate the development of more realistic dynamic ECM models for various of biological and medical applications. 22,23 The switching mechanism on electroactive polymer surfaces is believed to be based on conformational changes between a collapsed and fully extended protein structure (Fig. 4d).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, it can be assumed that protein switching is not merely a binary operation but instead sensitive to several factors including media composition, in particular its protein concentration and buffer composition, as well as protein immobilization density, which ultimately induce steric hindrances and thereby affect switching efficiency. 22,23 Experimental section…”

Section: Discussionmentioning

confidence: 99%

“…19,20 In this regard, stimuli such as temperature, light, magnetic field and electrical potential can alter and manipulate the surface properties of a film and thus change and control availability, function or activity of immobilized biomolecules on the surfaces, thereby representing promising tools for biomedical and biotechnological applications, especially to develop dynamic surfaces that can mimic in vivo situations. [21][22][23] The object of this study was to establish an in vitro system or microphysiological device for presentation of membrane-bound cytokines using electroactive PPy surfaces that will allow for dissecting the involved mechanisms and identifying the cellular mediators that potentially regulate stem cell survival, self-renewal and proliferation. Moreover, to mimic biological stimuli, the electroactive properties of the PPy film will be used to establish a switchable presentation of the immobilized cytokine of interest based on alterations in redox potential.…”

Section: Introductionmentioning

confidence: 99%

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Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells

2018

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“…Having established very high switching efficiencies in such media, we were interested to investigate whether the switchable surfaces that rely on electrically induced conformational changes can be used in more complex biological conditions. Different media commonly used for cell and tissue culture were investigated, 12 namely, Dulbecco’s modified Eagle’s medium (DMEM), DMEM containing 10% fetal bovine serum (DMEM-FBS), and DMEM-FBS with (4-(2-hydroxyethyl)-1-piperazineethanessulfonic acid) HEPES buffer (DMEM-FBS-HEPES). DMEM contains a mixture of inorganic salts, amino acids, glucose, and vitamins.…”

Section: Performance Under Complex Biological Conditionsmentioning

confidence: 99%

Electrically Responsive Surfaces: Experimental and Theoretical Investigations

Cantini¹,

Wang

Koelsch

et al. 2016

Acc. Chem. Res.

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ConspectusStimuli-responsive surfaces have sparked considerable interest in recent years, especially in view of their biomimetic nature and widespread biomedical applications. Significant efforts are continuously being directed at developing functional surfaces exhibiting specific property changes triggered by variations in electrical potential, temperature, pH and concentration, irradiation with light, or exposure to a magnetic field. In this respect, electrical stimulus offers several attractive features, including a high level of spatial and temporal controllability, rapid and reverse inducement, and noninvasiveness. In this Account, we discuss how surfaces can be designed and methodologies developed to produce electrically switchable systems, based on research by our groups. We aim to provide fundamental mechanistic and structural features of these dynamic systems, while highlighting their capabilities and potential applications. We begin by briefly describing the current state-of-the-art in integrating electroactive species on surfaces to control the immobilization of diverse biological entities. This premise leads us to portray our electrically switchable surfaces, capable of controlling nonspecific and specific biological interactions by exploiting molecular motions of surface-bound electroswitchable molecules. We demonstrate that our self-assembled monolayer-based electrically switchable surfaces can modulate the interactions of surfaces with proteins, mammalian and bacterial cells. We emphasize how these systems are ubiquitous in both switching biomolecular interactions in highly complex biological conditions while still offering antifouling properties. We also introduce how novel characterization techniques, such as surface sensitive vibrational sum-frequency generation (SFG) spectroscopy, can be used for probing the electrically switchable molecular surfaces in situ. SFG spectroscopy is a technique that not only allowed determining the structural orientation of the surface-tethered molecules under electroinduced switching, but also provided an in-depth characterization of the system reversibility. Furthermore, the unique support from molecular dynamics (MD) simulations is highlighted. MD simulations with polarizable force fields (FFs), which could give proper description of the charge polarization caused by electrical stimulus, have helped not only back many of the experimental observations, but also to rationalize the mechanism of switching behavior. More importantly, this polarizable FF-based approach can efficiently be extended to light or pH stimulated surfaces when integrated with reactive FF methods. The interplay between experimental and theoretical studies has led to a higher level of understanding of the switchable surfaces, and to a more precise interpretation and rationalization of the observed data. The perspectives on the challenges and opportunities for future progress on stimuli-responsive surfaces are also presented.

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Switching specific biomolecular interactions on surfaces under complex biological conditions

Abstract: Electrically switchable surfaces based on oligopeptides are ubiquitous in both switching specific protein interactions in highly fouling media while still offering the non-specific protein-resistance to the surface.

Cited by 21 publications

References 46 publications

Synthesis conditions influencing formation of MAPbBr3 perovskite nanoparticles prepared by the ligand-assisted precipitation method

Synthesis conditions influencing formation of MAPbBr3 perovskite nanoparticles prepared by the ligand-assisted precipitation method

Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells

Electrically Responsive Surfaces: Experimental and Theoretical Investigations

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