Switching to external flows: perturbations of developing vasculature within chicken chorioallantoic membrane

Padmanaban, Prasanna; van Galen, Danny; Salehi-Nik, Nasim; Zakharova, Mariia; Segerink, Loes; Rouwkema, Jeroen

doi:10.1039/d4lc00311j

Cited by 1 publication

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“…Recent studies show that mechanical forces like pressure [21,22] , compression [4,23,24] , and stretch are crucial in molding the vascularized tissue shapes. Recently, we have demonstrated that the development and organization of vascular networks during embryogenesis are adaptable to flow-induced shear stresses and can be altered by externally induced flows [25,26] . In engineered microtissues, local mechanical strain emerging from compaction and cellular traction forces on the extracellular environment induce tissue deformation, leading to changes in topology and organization of vasculature [8] .…”

Section: Introductionmentioning

confidence: 99%

Shape that matters: Yolk geometry spatially modulates developing vascular networks within chick chorioallantoic membrane

Padmanaban,

Wanders,

Katovich

et al. 2024

Preprint

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Controlling the multiscale organization of vasculature within diverse geometries is essential for shaping tissue-specific and organ-specific architectures. Nevertheless, how geometrical characteristics of surrounding tissues influence vessel morphology and blood flow remains unclear. Where the regulation of vascular organization by mechanical signals associated with fluid flow is well known, this study postulates that the organization of developing vasculature can also be regulated by mechanical signals connected to the confinement and thus the deformation of surrounding tissues. To test the Shape-Induced Vascular Adaptation (SIVA) concept, fertilized chicken egg contents containing developing vasculature were cultured within engineered eggshell platforms of different shapes. Our findings demonstrate that the vascularized chick chorioallantoic membrane (CAM) adapts to the shape of engineered eggshell, long before reaching its boundaries. This adaptation affects the organization of the vascular network within the CAM, affecting parameters such as vessel area, branching, orientation, length, diameter and endpoints. Specifically, we observed that sharp corners in the engineered eggshell led to more elongated vascular structures. To further explore the dynamic nature of this phenomenon, a proof-of-concept experiment was performed using a shape-shifting engineered eggshell that deforms the egg content from circle to square shape. Using this shape-shifting prototype, we observed a direct effect of eggshell deformation on the vessel morphology and flow dynamics in a time-dependent manner. Overall, our ex-ovo experimental platform provides a unique opportunity to study how mechanical stimuli such as shape influence the spatial and temporal organization of developing vascularized tissues. By subjecting these tissues to various static and dynamic conditions, we induced both local and global changes in their organization. This class of perturbation provides us with an additional tool which can be used for shaping vascular organization within developing tissues and to engineer tissues with geometrically tunable vessel structures.

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Section: Introductionmentioning

confidence: 99%

Shape that matters: Yolk geometry spatially modulates developing vascular networks within chick chorioallantoic membrane

Padmanaban,

Wanders,

Katovich

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

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Switching to external flows: perturbations of developing vasculature within chicken chorioallantoic membrane

Abstract: The impact of fluid flow shear stresses, generated by the movement of blood through vasculature, on the organization and maturation of vessels is widely recognized. Nevertheless, it remains uncertain whether...

Cited by 1 publication

References 29 publications

Shape that matters: Yolk geometry spatially modulates developing vascular networks within chick chorioallantoic membrane

Shape that matters: Yolk geometry spatially modulates developing vascular networks within chick chorioallantoic membrane

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