SxIP binding disrupts the constitutive homodimer interface of EB1 and stabilizes EB1 monomer

Kinetochore-microtubule attachment in human cells is regulated by the interaction of a conserved motif of Ska1 with EB1

“…As expected, 6xHis Ska1Δloop also did not show any association with EB1 GST. Previous studies developed a SXIP peptide aptamer (ALNGQSRIPVLKRHTR) that binds to EB1 strongly and interferes with plus end targeting of several EB1-binding proteins ( 46 , 47 ). Therefore, we also checked if the EB1-binding SXIP peptide aptamer interferes with Ska1–EB1 interaction.…”

“…S4 , C and D ). Interestingly, the residues affected by Ska1 SHLP peptide are nearly the same subset of amino acids that showed large chemical shift deviations upon binding of EB1 to the SXIP aptamer peptide ( 47 ) and they are conserved across several metazoan species ( Fig. 5 H ).…”

“…The SXIP-docking sites of nearly all the SXIP +TIPs lie within the EBH domain of EB1 C-terminus ( 36 , 47 , 55 ). Usually, the apolar I and P residues of SXIP are involved in packing interaction with the hydrophobic cleft of EBH and mutation of these sites to polar residues, such as Asparagine largely interferes with EB1 binding ( 36 , 42 ).…”