SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway

Jor, E.; Myrmel, Mette; Jonassen, Christine Monceyron

doi:10.1016/j.jviromet.2010.03.028

Cited by 32 publications

(38 citation statements)

References 48 publications

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“…A high frequency of BNoV detection is, on the other hand, not a surprise since many other investigators have previously reported a high prevalence of BNoV infection in the studied bovine populations Di Bartolo et al, 2011;Jor et al, 2010;Kaplon et al, 2011;Mijovski et al, 2010;Park et al, 2007;Reuter et al, 2009;van der Poel et al, 2003;Wise et al, 2004;Yilmaz et al, 2011). Clinical significance of BNoV infection has not been clear in the field because the virus has also been found in clinically healthy calves (Jor et al, 2010;Mijovski et al, 2010) as also shown in our study. Recently an animal study has demonstrated that BNoV is pathogenic to naïve calves (Otto et al, 2011).…”

Section: Discussionsupporting

confidence: 64%

“…For detection of Nebovirus, a gel-based nested RT-PCR was used as previously described (Jor et al, 2010). The PCR was conducted using OneStep RT-PCR Kit (QIAGEN, Valencia, CA) with QIAGEN 1 RNase inhibitor and HotStarTaq 1 DNA Polymerase Kit (QIAGEN, Valencia, CA) for RT-PCR and nested PCR, respectively, according to the manufacturer's instruction.…”

Section: Detection Of Pathogensmentioning

confidence: 99%

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Case–control study of microbiological etiology associated with calf diarrhea

Cho

Han

Wang

et al. 2013

Veterinary Microbiology

141

132

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Calf diarrhea is a major economic burden for the US cattle industry. A variety of infectious agents are implicated in calf diarrhea and co-infection of multiple pathogens is not uncommon in diarrheic calves. A case-control study was conducted to assess infectious etiologies associated with calf diarrhea in Midwest cattle farms. A total of 199 and 245 fecal samples were obtained from diarrheic and healthy calves, respectively, from 165 cattle farms. Samples were tested by a panel of multiplex PCR assays for 11 enteric pathogens: bovine rotavirus group A (BRV-A), bovine coronavirus (BCoV), bovine viral diarrhea virus (BVDV), bovine enterovirus (BEV), bovine norovirus (BNoV), Nebovirus, bovine torovirus (BToV) Salmonella spp. (Salmonella), Escherichia coli (E. coli) K99(+), Clostridium perfringens with β toxin gene and Cryptosporidium parvum (C. parvum). The association between diarrhea and detection of each pathogen was analyzed using a multivariate logistic regression model. More than a half of the fecal samples from the diarrheic calves had multiple pathogens. Statistically, BRV-A, BCoV, BNoV, Nebovirus, Salmonella, E. coli K99(+), and C. parvum were significantly associated with calf diarrhea (p<0.05). Among them, C. parvum and BRV-A were considered to be the most common enteric pathogens for calf diarrhea with high detection frequency (33.7% and 27.1%) and strong odds ratio (173 and 79.9). Unexpectedly BNoV (OR=2.0) and Nebovirus (OR=16.7) were identified with high frequency in diarrheic calves, suggesting these viruses may have a significant contribution to calf diarrhea.

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Section: Discussionsupporting

confidence: 64%

Section: Detection Of Pathogensmentioning

confidence: 99%

Case–control study of microbiological etiology associated with calf diarrhea

Cho

Han

Wang

et al. 2013

Veterinary Microbiology

141

132

View full text Add to dashboard Cite

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“…Identical information about the genotypic profile of the samples were observed in electropherograms obtained by sequencing, which highlights the efficiency and reliability of PCR and electrophoresis on 3% agarose gel for genotyping and analysis of polymorphisms in these regions of the PRNP gene (Fig.4 and Fig.5). Genotyping can also be performed by real-time PCR (Jor et al 2010) or even by means of mass spectrometry, however these methods show is expensive when compared to conventional PCR coupled with agarose gel electrophoresis and these techniques have equivalent efficiency to conventional PCR and Sanger sequencing.…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms in the Prion Protein Gene of cattle breeds from Brazil

et al. 2016

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One of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms.

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“…This strain had a GIII.1-related RdRp sequence and a GIII.2-related capsid coding sequence. Molecular investigations from diverse geographical settings have revealed the circulation of a number of recombinant strains genetically related to Bo/Thirsk10/00/UK (Han et al, 2004;Mauroy et al, 2009b;Jor et al, 2010;Di Martino et al, 2014a). In contrast, only one strain, B-1SVD/03/US, was identified with a GIII.2-related RdRp and a GIII.1related capsid coding sequence (Bull et al, 2007) (Fig.…”

Section: Recombination Of Norovirusesmentioning

confidence: 99%

“…BoNoVs have been detected using molecular methods in faecal samples of diarrhoeic calves, either alone or as co-infections with other enteric viruses, such as rotavirus, nebovirus, coronavirus and bovine viral diarrhoea virus (BVDV) (Smiley et al, 2003;Park et al, 2007;Mauroy et al, 2009a;Jor et al, 2010;Di Bartolo et al, 2011;Cho et al, 2013). It is possible that mixed infections influence the severity of BoNoV infections.…”

Section: Pathogenesis and Clinical Features Of Bovine Norovirus Infecmentioning

confidence: 99%

Bovine noroviruses: A missing component of calf diarrhoea diagnosis

Felice

Mauroy

Pozzo

et al. 2016

The Veterinary Journal

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Noroviruses are RNA viruses that belong to the Genus Norovirus, Family Caliciviridae, and infect human beings and several animal species, including cattle. Bovine norovirus infections have been detected in cattle of a range of different ages throughout the world. Currently there is no suitable cell culture system for these viruses and information on their pathogenesis is limited. Molecular and serological tests have been developed, but are complicated by the high genetic and antigenic diversity of bovine noroviruses. Bovine noroviruses can be detected frequently in faecal samples of diarrhoeic calves, either alone or in association with other common enteric pathogens, suggesting a role for these viruses in the aetiology of calf enteritis.

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SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway

Cited by 32 publications

References 48 publications

Case–control study of microbiological etiology associated with calf diarrhea

Case–control study of microbiological etiology associated with calf diarrhea

Polymorphisms in the Prion Protein Gene of cattle breeds from Brazil

Bovine noroviruses: A missing component of calf diarrhoea diagnosis

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