Symbiotic bacteria contribute to increasing the population size of a freshwater crustacean, <scp><i>D</i></scp><i>aphnia magna</i>

Peerakietkhajorn, Saranya; Tsukada, Koji; Kato, Yasuhiko; Matsuura, Tomoaki; Watanabe, Hidenobu

doi:10.1111/1758-2229.12260

Cited by 46 publications

(58 citation statements)

References 51 publications

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“…β-proteobacteria (mostly Comamonadaceae) were over-represented in GM in Experiment 1 (p < 0.05 by Wald test; Supplementary Table S1a). The Comamonadaceae family is a major constituent of the microbiome and induces positive fitness effects in Daphnia (Eckert and Pernthaler, 2014;Peerakietkhajorn et al, 2015;Macke et al, 2017a;Callens et al, 2018;Mushegian et al, 2019). In Experiment 2, in which Daphnia were transferred into sterile water following the transplant so that BPK could PERMANOVA were performed on Bray-Curtis dissimilarity indices for BPK and GM simultaneously (a), BPK only (b), and GM only (c).…”

Section: Resultsmentioning

confidence: 99%

Diet and Genotype of an Aquatic Invertebrate Affect the Composition of Free-Living Microbial Communities

et al. 2020

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In spite of the growing interest in the role of the gut microbiome (GM) in host physiology and health, the mechanisms governing its assembly and its effects on the environment are poorly understood. In this article, we show that the host genotype and the GM of Daphnia influence the community structure of the surrounding bacterioplankton (BPK). When Daphnia genotypes were placed in an identical environment, both the GM and BPK showed a genotype and diet-dependent taxonomic composition. Overall, the GM strongly differed from the BPK in taxonomic composition and was characterized by a lower α-diversity, suggesting a selective rejecting of bacteria from the regional species pool. In a microbiome transplant experiment, the assembly of both the GM and BPK was strongly affected by the host genotype and the inoculum to which germ-free Daphnia were exposed. The combination of these results suggests a strong interaction between the host genotype, its GM and free-living microbial communities. Currently, it is generally assumed that an animal's diet has a strong effect on the animal's GM, but only a negligible (if any) effect on the surrounding environment. However, our results indicate that the diet/microbiome inocula have a small effect on the gut community and a large effect on the community in the surrounding environment. This structuring genotype × microbiome × environment effect is an essential prerequisite that could indicate that microbiomes play an important role in eco-evolutionary processes.

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Section: Resultsmentioning

confidence: 99%

Diet and Genotype of an Aquatic Invertebrate Affect the Composition of Free-Living Microbial Communities

et al. 2020

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“…It was also reported that specific bacterial taxa were consistently found in affiliation with Daphnia, even in geographically separated populations with different genetic backgrounds (Qi et al, 2009). Particularly, the genus Limnohabitans (β-proteobacteria) was a dominant constituent of the Daphnia microbiomes (Freese and Schink, 2011;Eckert and Pernthaler, 2014;Peerakietkhajorn et al, 2015;Callens et al, 2016). Other taxa also reported in microbiome studies with Daphnia were Flavobacterium, Rhodobacter, Chromobacterium, Methylibium, Bordetella, Burkholderia, and Cupriavidus (Qi et al, 2009).…”

Section: Introductionmentioning

confidence: 92%

“…There is no cultured representative of this CL500-29 clade, but enrichment experiments with seawater suggest that this group can utilize different carbon sources aerobically (McIlroy et al, 2017). Limnohabitans (β-proteobacteria) is responsible for dissolved organic carbon (DOC) flux (Eckert and Pernthaler, 2014) and these bacteria are widely observed in zooplankton indicating their potential symbiotic lifestyle although they also occur free-living (Peerakietkhajorn et al, 2015). Release of ammonium by zooplankton may also explain occurrence of N-cycling taxa, several taxa related to alpha-, beta-, and gamma-proteobacteria contribute to nitrification as well as denitrification (Peura et al, 2015).…”

Section: Indicator Species and Their Potential Link To Nutrient Cyclingmentioning

confidence: 99%

Niche Differentiation of Host-Associated Pelagic Microbes and Their Potential Contribution to Biogeochemical Cycling in Artificially Warmed Lakes

et al. 2020

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“…Previous studies on aposymbiotic daphnids showed that disruption in gut microbiota, either by drugs or diet, had adverse effects on nutrition (40) (11), immunity (8), growth (12), fecundity (22), and longevity (47). The effects that we observed however, were most prominent at low antibiotic concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…It is a common view that strains capable of supplying essential elements for reproduction and growth would benefit the host. For example, the key components of Daphnia gut microbiota, Limnohabitans, Aeromonas and methanotrophic bacteria (47), have been linked to acquisition of essential amino acids (58,38), polyunsaturated fatty acids (PUFA) and sterols (60) that positively affect Daphnia growth and reproduction (9,61). Surprisingly, none of these taxa were associated with elevated growth and fecundity in our study.…”

Section: Discussionmentioning

confidence: 99%

Disparate effects of antibiotic-induced microbiome change and enhanced fitness inDaphnia magna

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Brindefalk

Ogonowski

et al. 2019

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13It is a common view that an organism's microbiota has a profound influence on host fitness; 14 however, supporting evidence is lacking in many organisms. We manipulated the gut 15 microbiome of Daphnia magna by chronic exposure to different concentrations of the 16 antibiotic Ciprofloxacin (0.01 -1 mg L -1 ), and evaluated whether this affected the animals' 17 fitness and antioxidant capacity. In line with our expectations, antibiotic exposure altered the 18 microbiome in a concentration-dependent manner. However, contrary to these expectations, 19 the reduced diversity of gut bacteria was not associated with any fitness detriment. Moreover, 20 the growth-related parameters correlated negatively with diversity indices; and, in the 21 daphnids exposed to the lowest ciprofloxacin concentrations, the antioxidant capacity, 22 growth, and fecundity were even higher than in control animals. These findings suggest that 23 ciprofloxacin exerts direct stimulatory effects on growth and reproduction in Daphnia, while 24 microbiome-mediated effects are of lesser importance. Thus, although microbiome profiling 25 of Daphnia may be a sensitive tool to identify early effects of antibiotic exposure, 26 disentangling direct and microbiome-mediated effects on host fitness is not straightforward. 27 68 sequencing (22 , 23). Regardless of the sequencing platform, origin of specimens, and culture 69 conditions, the core microbiome appears relatively stable, mainly comprised of 70 Betaproteobacteria, Gammaproteobacteria and facultative anaerobic Bacteroidetes species. 71At the genus level, Limnohabitans has been reported as one of the most stable and dominant 72 members in Daphnia gut, and variations in its abundance have been tied to the animal 73 fecundity (22). Although some studies have addressed the dependence of Daphnia on its 74 microbiota (9) and some short-term effects on fitness following exposure to antibiotics have 75 been observed in Daphnia magna (25, 13), the relationship between microflora perturbation 76 and host fitness is still unclear, as is the involvement and modulating role of antioxidants in 77 these relationships. 78In this study, the relationship between antibiotic-mediated gut microbiome modulation and 79 host fitness were addressed experimentally using a model cladoceran Daphnia magna. We 80 monitored changes in the gut microbiome, host longevity, growth, and reproduction, as well 81 as antioxidant levels in the exposed animals following ciprofloxacin exposure. We 82 hypothesized that the diversity and abundance of the gut-associated microflora would 83 decrease with increasing concentration of antibiotics. Furthermore, we expected longer 84 exposure time and higher antibiotic concentrations to have negative effects on somatic 85 growth, reproductive output, and antioxidant capacity. These reductions we expected would 86 be due to reduced bacterial diversity in particular, and to some extent, an altered community 87 composition. These hypotheses were tested by combining (1) long-term (21 d) exposure...

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Symbiotic bacteria contribute to increasing the population size of a freshwater crustacean, Daphnia magna

Cited by 46 publications

References 51 publications

Diet and Genotype of an Aquatic Invertebrate Affect the Composition of Free-Living Microbial Communities

Diet and Genotype of an Aquatic Invertebrate Affect the Composition of Free-Living Microbial Communities

Niche Differentiation of Host-Associated Pelagic Microbes and Their Potential Contribution to Biogeochemical Cycling in Artificially Warmed Lakes

Disparate effects of antibiotic-induced microbiome change and enhanced fitness inDaphnia magna

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