Symbiotic bacteria (Erwinia sp.) in the gut of Frankliniella occidentalis (Thysanoptera: Thripidae) do not affect its ability to transmit tospovirus

Vries, E.J. de; Wetering, F. van de; Hoek, M.M. van der; Jacobs, G.; Breeuwer, Johannes A. J.

doi:10.14411/eje.2012.034

Cited by 8 publications

(8 citation statements)

References 27 publications

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“…Erwinia, Pantoea, Enterobacter, Serratia, Escherichia and Pseudomonas have been identified internally from thrips (de Vries et al 2001a; Gitaitis et al 2003; Chanbusarakum & Ullman 2008; de Vries et al 2008; de Vries et al 2012). Erwinia herbicola and Enterobacter agglomerans have been synonymized with Pantoea agglomerans so these 3 genera identified in chilli thrips could represent a single taxon found in association with several insects (Medina et al 2011).…”

Section: Resultsmentioning

confidence: 99%

“…Thrips on the whole have received little attention regarding this important aspect of arthropod ecology. Previous work has largely focused on the most frequently encountered bacterium (identified as a near- Erwinia species) within a single thrips species, the Western flower thrips Frankliniella occidentalis (de Vries et al 2001a; de Vries et al 2001b; de Vries et al 2004; de Vries et al 2006; Chanbusarakum & Ullman 2008, 2009; de Vries et al 2012) with a few studies on other species (Wells et al 2002; Gitaitis et al 2003; de Vries et al 2008). …”

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confidence: 99%

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Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Dickey¹,

Trease²,

Jara-Cavieres³

et al. 2014

Florida Entomologist

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The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Dickey¹,

Trease²,

Jara-Cavieres³

et al. 2014

Florida Entomologist

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“…The different genomic components of the thrips holobiont are beginning to be unveiled: recently, the genome of WFT and that of the melon thrips ( T. palmi ) were characterized ( 37 , 38 ); the bacteria associated with thrips were also partially characterized in the case of WFT ( 39 – 41 ). Regarding the viral component of the thrips microbiome, knowledge is still very limited: a recent work described viruses associated with soybean thrips samples from various U.S. regions ( 16 ); furthermore, four genomovirus genomes were amplified from Echinothrips americanus (Morgan), a pest thrips sampled in Florida ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Complexity and Local Specificity of the Virome Associated with Tospovirus-Transmitting Thrips Species

Chiapello

Bosco

Ciuffo

et al. 2021

J Virol

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Frankliniella occidentalis (western flower thrips=WFT) and Thrips tabaci (onion thrips=OT) are insect species that greatly impact horticultural crops through direct damage and their efficient vectoring of tomato spotted wilt virus and iris yellow spot virus. In this study we collected thrips of these species from 12 field populations in various regions in Italy. We also included one field population of Neohydatothrips variabilis (soybean thrips=ST) from the U.S.A. Total RNAseq from high-throughput sequencing (HTS) was used to assemble the virome and then we assigned putative viral contigs to each thrips sample by qRT-PCR. Excluding plant and fungal viruses, we were able to identify 61 viral segments, corresponding to 41 viruses: 14 were assigned to WFT, 17 to OT, one from ST and 9 viruses could not be assigned to any species based on our stringent criteria. All these viruses are putative representative of new species (with only the exception of a sobemo-like virus that is 100% identical to a virus recently characterized in ST) and some belong to new higher-ranking taxa. These additions to the viral phylogeny suggest previously undescribed evolutionary niches. Most of the Baltimore’s classes of RNA viruses were present (positive- and minus- strand and dsRNA viruses), but only one DNA virus was identified in our collection. Repeated sampling in a subset of locations in 2019 and 2020 and further virus characterization in a subset of four thrips populations maintained in laboratory allowed us to provide evidence of a locally persistent thrips core virome that characterizes each population. IMPORTANCE Harnessing the insect microbiome can result in new approaches to contain their populations or the damage they cause vectoring viruses of medical, veterinary, or agricultural importance. Persistent insect viruses are a neglected component of their microbiota. Here for the first time, we characterize the virome associated with the two model systems for tospovirus-transmitting thrips species, of utmost importance for the direct and indirect damage they cause to a number of different crops. The thrips virome here characterized includes several novel viruses, that in some cases reveal previously undescribed clades. More importantly, some of the viruses we describe are part of a core virome that is specific and consistently present in distinct geographical locations monitored over the years, hinting at a possible mutualistic symbiotic relationship with their host.

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“…occidentalis and T . tabaci 30 , 32 , 34 , 35 , 73 , while Agrobacterium , Methylobacterium and Pseudomonas have been identified from S . staphylinus 90 and S .…”

Section: Discussionmentioning

confidence: 99%

“…Thrips on the whole have received relatively little attention regarding their bacterial communities. Previous studies have largely focused on the most frequently encountered bacterium (identified as a near- Erwinia species) within a single thrips species Frankliniella occidentalis 28 – 32 with a few studies on other species (i.e. Aptinothrips species 33 , Thrips tabaci 34 , Scirtothrips dorsalis 35 , Frankliniella fusca 36 and F .…”

Section: Introductionmentioning

confidence: 99%

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Kaczmarczyk

Kucharczyk

et al. 2018

Sci Rep

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Insects’ exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial communities of four developmental stages of the fungivorous species Hoplothrips carpathicus (Thysanoptera: Phlaeothripidae), verification of the presence of Wolbachia, in silico prediction of metabolic potentials of the microorganisms, and sequencing its mitochondrial COI barcode. Taxonomy-based analysis indicated that the bacterial community of H. carpathicus contained 21 bacterial phyla. The most abundant phyla were Proteobacteria, Actinobacteria, Bacterioidetes and Firmicutes, and the most abundant classes were Alphaproteobacteria, Actinobacteria, Gammaproteobacteria and Betaproteobacteria, with different proportions in the total share. For pupa and imago (adult) the most abundant genus was Wolbachia, which comprised 69.95% and 56.11% of total bacterial population respectively. Moreover, similarity analysis of bacterial communities showed that changes in microbiome composition are congruent with the successive stages of H. carpathicus development. PICRUSt analysis predicted that each bacterial community should be rich in genes involved in membrane transport, amino acid metabolism, carbohydrate metabolism, replication and repair processes.

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Symbiotic bacteria (Erwinia sp.) in the gut of Frankliniella occidentalis (Thysanoptera: Thripidae) do not affect its ability to transmit tospovirus

Cited by 8 publications

References 27 publications

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Complexity and Local Specificity of the Virome Associated with Tospovirus-Transmitting Thrips Species

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

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