Symbiotic competence, genetic diversity and plasmid profiles of Egyptian isolates of a Rhizobium species from Leucaena leucocephala (Lam.) Dewit

Kuykendall, L. D.; Swelim, D. M.; Hashem, Fawzy; Abdelwahab, Sherif; Hegazi, N. A.

doi:10.1111/j.1472-765x.1996.tb01176.x

Cited by 8 publications

(7 citation statements)

References 13 publications

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“…Rhizobia associating with this plant have been isolated from South America, Asia, Africa, and Australia. Some of these isolates have been classified as belonging to the species Rhizobium tropici (Martínez‐Romero et al 1991) and Mesorhizobium plurifarium (De Lajudie et al 1998), while others remain unnamed (Trinick 1980; Jarvis 1983; Martínez et al 1985; Wedlock & Jarvis 1986; Zhang et al 1991; Oyaizu et al 1993; De Lajudie et al 1994; Gao et al 1994; Kuykendall et al 1996). Strains originally isolated from other host species can also nodulate L. leucocephala, for example R. gallicum and R. giardinii isolated from common bean (Amarger et al 1997) , R. huautlense from Sesbania herbacea (Wang et al 1998), and R. etli bv.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of rhizobia from Leucaena leucocephala nodules in Mexican soils

1999

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Leucaena species are leguminous plants native to Mexico. Using two L. leucocephala cultivars grown in different soils, we obtained 150 isolates from the nodules. Twelve rDNA types were identified which clustered into groups corresponding to Mesorhizobium, Rhizobium, and Sinorhizobium by restriction fragment length polymorphism (RFLP) of amplified 16S rRNA genes. Types 2, 4, 5, 6, 10, 11, and 12 were distinct from all the defined species. Others had patterns indistinguishable from some recognized species. Most of the isolates corresponded to Sinorhizobium. Forty‐one electrophoretic types (ETs) were identified among the isolates based on the different combinations of electrophoretic patterns of 13 metabolic enzymes. ETs were clustered into groups in general agreement with the rDNA types. Diverse plasmid patterns were obtained among the isolates, but common plasmids were observed among most isolates within rDNA types 5, 10, and 11. The symbiotic plasmids were identified among most of the isolates, except for the Mesorhizobium isolates. The affinities of host cultivars for different rhizobial groups and the impact of soil cultivation on the soil populations of rhizobia were analysed from the estimation of isolation frequencies and diversity. The results showed differences in rhizobial populations in cultivated and uncultivated soils and also differences in rhizobia trapped by L. leucocephala cv. Cunningham or Peruvian.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of rhizobia from Leucaena leucocephala nodules in Mexican soils

1999

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“…The Rhizobium strains, chosen to represent the maximum available genetic diversity (Kuykendall et al 1996), were DS 3, DS 9, DS 43, DS 65, DS 78, DS 91, DS 93, DS 129, DS 137, DS 144/1, DS 144/2, DS 157 and DS 158. These strains are part of the ARC Culture Collection in Egypt and were originally isolated from root nodules of L. leucocephala grown in different regions of Egypt.…”

Section: Rhizobium Strains and Culture Conditlonamentioning

confidence: 99%

“…(Leucuenu) strains varied substantially. These same strains were characterized for plasmid profile and nifgene localization (Hashem and Kuykendall 1994) and for genetic diversity and symbiotic competence (Kuykendall et al 1996).…”

Section: Competition Between Wild-type and Marked Strainsmentioning

confidence: 99%

Competitiveness of Rhizobium sp. [Leucaena leucocephala (Lam.) Dewit] strains and their genetically marked derivatives

Swelim

Kuykendall

Hashem

et al. 1996

Lett Appl Microbiol

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D.M. SWELIM, L.D. KUYKENDALL, F.M. HASHEM, S.M. ABDEL‐WAHAB AND N.I. HEGAZI. 1996. The competitiveness of wild‐type strains of Rhizobium sp. (Leucaena) and their genetically marked double mutants was examined in mixed infection experiments in the greenhouse. Antibiotic resistance markers were selected for use in strain identification, but these genetic markers apparently lowered both competitiveness and effectiveness, except in the case of strain DS 144/2 where the genetically marked derivative was evidently superior to the wild‐type parent strain in effectiveness. Four wild‐type strains and their genetically marked derivatives were carefully evaluated using double reciprocal pairs, the results of which nevertheless allowed the formulation of some conclusions. Strains DS 65 and DS 78 were more competitive than strain DS 144/2; only strain DS 78 was more competitive than DS 158; and strains DS 158 and DS 65 were equally competitive. There was no correlation between nodule number and competitiveness. Shoot dry weight and nitrogen mass, as well as nitrogenase activity, decreased with some strain mixtures indicating that relatively ineffective symbioses had formed, as compared with single‐strain inoculations using symbiotically competent strains.

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“…Specifically, bacteriophage susceptibility (Kankila and Lindstrom, 1994;Werquin et al, 1988) and plasmid DNA content have proven to be useful tools for differentiating Rhizobium strains and studying their ecology (Brockman and Bezdicek, 1989;Buendia-Claveria et al, 1989;Hartmann and Amarger, 1991;H ashem and Kuykendall, 1994;Kuykendall et al, 1996;Moenne-Loccoz et al, 1994;Shishido and Pepper, 1990;Zahran, 1992). Their ability to promote genetic exchange, or transduction, between host strains has also been useful (Kinkle and Schmidt, 1991;Pretorius-Guth et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…leguminosarum bv. trifolii (Hashem and Kuykendall, 1994;Hynes and McGregor, 1990), R. meliloti (Hynes et al, 1986;Hashem and Kuykendall, 1994), and Rhizobium species that nodulate Leucaena (Hashem and Kuykendall, 1994;Kuykendall et al, 1996). In addition to symbiotic plasmids, Rhizobium strains may carry between one and ten cryptic plasmids (Hashem and Kuykendall, t994;Thurman et al, 1985) ranging in size from < 30 MDa to > 1000 MDa Hashem and Kuykendall, 1994).…”

Section: Introductionmentioning

confidence: 99%

Phage susceptibility and plasmid profile analysis of Sinorhizobium fredii

Hashem¹,

Kuykendall²,

Udell³

et al. 1996

Plant Soil

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This is the first report identifying bacteriophages and documenting megaplasmids of Sinorhizobiumfredii. Plasmid DNA content and bacteriophage typing of eighteen strains of S. fredii were determined. S. fredii strains fell into ten plasmid profile groups containing 1 to 6 plasmids, some evidently larger than 1000 MDa. Twenty-three S. fredii lytic phages were isolated from soil, and they lysed six different S. fredii strains. The host range and plaque morphology of these phages were studied. Susceptibility to S. fredii phages was examined for S. meliloti; Rhizobium leguminosarum bvs. viceae, trifolii and phaseoli; R. loti; Bradyrhizobium japonicum; B. elkanii and Bradyrhizobium sp. (Arachis). Several phages that originally lysed S. fredii strain USDA 206 also lysed strains of all three S. fredii serogroups described originally by Sadowsky et al. Phages that infected S. fredii strains USDA 191 and USDA 257 were highly specific and lysed only serogroup 193 strains. S. meliloti strains L5-30 and USDA 1005 were lysed by three of the phages that lysed S. fredii strain USDA 217. No other Rhizobium or Bradyrhizobium strain tested was susceptible to lysis by any of the S. fredii phages. The present investigation indicates that phage susceptibility in conjunction with plasmid profile analysis may provide a rapid method for identification and characterization of strains of S. fredii.

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Symbiotic competence, genetic diversity and plasmid profiles of Egyptian isolates of a Rhizobium species from Leucaena leucocephala (Lam.) Dewit

Cited by 8 publications

References 13 publications

Genetic diversity of rhizobia from Leucaena leucocephala nodules in Mexican soils

Genetic diversity of rhizobia from Leucaena leucocephala nodules in Mexican soils

Competitiveness of Rhizobium sp. [Leucaena leucocephala (Lam.) Dewit] strains and their genetically marked derivatives

Phage susceptibility and plasmid profile analysis of Sinorhizobium fredii

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