Symbiotic efficiency and genetic characteristics of Bradyrhizobium sp. strain UFSM LA 1.3 isolated from Lupinus albescens (H. et Arn)

Abstract

Legume species belonging to the genus Lupinus are annual herb plants. The majority of them are indigenous to the Americas. They are known for nitrogen-fixing symbioses with soil bacteria collectively called rhizobia. The aim of this study was to characterize a rhizobium strain isolated from Lupinus albescens using phenotypic, symbiotic and molecular approaches. Strain UFSM LA 1.3 was tested in vitro according to several parameters: colony size, color and growing rate; acid or alkaline reaction in yeast mannitol media supplemented with bromothymol blue; gum production. Molecular characterization was evaluated by PCR technique using primers BOX A1-R and sequence analysis of the 16S-23S rDNA intergenic region (ITS). ITS sequencing fragments showed genetic similarity with Bradyrhizobium sp. The polymorphism observed by BOX-PCR have shown that strain differs from the reference strain SEMIA 928 and SEMIA 938. The symbiotic efficiency under axenic conditions of UFSM LA 1.3 was 94.6%, without statistical differences compared to the mineral nitrogen fertilized control, to which was applied solution of 400 mg of ammonium nitrate.

How a phosphorus‐acquisition strategy based on carboxylate exudation powers the success and agronomic potential of lupines (Lupinus, Fabaceae)

“…& Arn. grows on coastal sand dunes and riverine sand banks in southern Brazil, Argentina, and Uruguay (Stroschein et al, 2010). In eastern North America, the four unifoliate lupines and, in eastern South America, lupines of the unifoliate group grow on very sandy nutrient‐poor soils (e.g., pine flatwoods in southeastern USA and South American cerrado savannas) that are also subject to frequent fires (Eastwood et al, 2008).…”

The genomes of three Bradyrhizobium sp. isolated from root nodules of Lupinus albescens grown in extremely poor soils display important genes for resistance to environmental stress

“…Adding the genome of the reference strain B. japonicum USDA 6 to the comparison ( Figure 3B ), genomes from AS23 and NAS80 resulted in a similar amount of genes shared with the reference genome (5,129 and 5,134, respectively), while the genome of isolate NAS96 shared 4,717 genes with the genome of USDA 6. Similarities among selected genes from Bradyrhizobium strains isolated from root nodules of L. albescens with the respective genes from B. japonicum species had already been observed by Stroschein et al (2010) and Granada et al (2015) . However, both works suggested that those bacterial isolates probably constituted new Bradyrhizobium species.…”

Quantitative Analysis of Performance and Stability of a Long and Thin Grain Rice Genotype for Rice-Growing Region of Michoacan, Mexico

“…O gênero Rhizobium, composto por microrganismos genericamente identificados como rizóbios, bactérias gram negativas, é geralmente relacionado a bactérias fixadoras de nitrogênio formadoras de nódulos em leguminosas (Stroschein, 2010). Singh et al (2015) isolaram Rhizobium radiobacter em milho, e também identificaram a capacidade de promoção de crescimento dessa bactéria.…”