Symbiotic efficiency of native rhizobia in legume tree Leucaena leucocephala derived from several soil classes of Brazilian Northeast region

Silva, Vinícius Santos Gomes da; Santos, Carolina Etienne de Rosália e Silva; Freitas, Ana Dolores Santiago de; Stamford, Newton Pereira; Silva, Aleksandro Ferreira da; Lyra, Maria do Carmo Catanho Pereira de; Santos, Leandro Reis Costa; Ferreira, Juscélia da Silva

doi:10.21475/ajcs.18.12.03.pne976

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…The observed noneffective (O), inhibitory (I) and effective (E) intraspecies interactions can thus be explained by the equilibrium between the energy cost of N 2 -fixation and mineral nitrogen acquisition [38]. Moreover, our results of SDW-based bio-typing are generally consistent with previous reports in regards to the use of plant dry matter biomass for evaluating symbiotic efficiency [35,39,40]. It is thus critically important to inoculate compatible rhizobial strains when a specific alfalfa cultivar is cultivated in agriculture.…”

Section: Biotype Identification On the Basis Of Symbiotic Performancesupporting

confidence: 90%

Plant transcriptome analysis reveals specific molecular interactions between alfalfa and its rhizobial symbionts below the species level

et al. 2020

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Background: Leguminous plants alter patterns of gene expression in response to symbiotic colonization and infection by their cognate rhizobial bacteria, but the extent of the transcriptomic response has rarely been examined below the species level. Here we describe the identification of 12 rhizobial biotypes of Ensifer meliloti, which form nitrogen-fixing nodules in the roots of alfalfa (Medicago sativa L.), followed by a comparative RNA-seq analysis of four alfalfa cultivars each inoculated with two E. meliloti strains varying in symbiotic performance and phylogenetic relatedness. Results: Rhizobial biotypes were identified on the basis of their symbiotic performance, particularly shoot dry weight. Differentially expressed genes (DEGs) and metabolic pathways were determined by comparing the RNA-seq data with that of the uninoculated control plant. Significant differences were found between DEGs generated in each cultivar with the inoculation of two rhizobial strains in comparison (P < 0.01). A total of 8111 genes was differentially expressed, representing~17.1% of the M. sativa genome. The proportion of DEGs ranges from 0.5 to 12.2% for each alfalfa cultivar. Interestingly, genes with predicted roles in flavonoid biosynthesis and plant-pathogen interaction (NBS-LRR) were identified as the most significant DEGs. Other DEGs include Medsa002106 and genes encoding nodulins and NCR peptides whose expression is specifically induced during the development of nitrogenfixing nodules. More importantly, strong significant positive correlations were observed between plant transcriptomes (DEGs and KEGG pathways) and phylogenetic distances between the two rhizobial inoculants. Conclusions: Alfalfa expresses significantly distinct sets of genes in response to infection by different rhizobial strains at the below-species levels (i.e. biotype or strain). Candidate genes underlying the specific interactions include Medsa002106 and those encoding nodulins and NCR peptides and proteins in the NBS-LRR family.

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Section: Biotype Identification On the Basis Of Symbiotic Performancesupporting

confidence: 90%

Plant transcriptome analysis reveals specific molecular interactions between alfalfa and its rhizobial symbionts below the species level

et al. 2020

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“…Shoot dry weight is used widely as a measure to reflect the symbiotic effectiveness of rhizobia (Somasegaran & Hoben, 1985). This is due to the role of nitrogen in influencing plant growth, dry matter and final yield (Rajput & Patil, 2017;Santos et al, 2018). The differences observed in shoot dry weight represents a different ability of rhizobia to influence nitrogen fixation through symbiotic relationship with their host plants (Thrall et al, 2011;Wielbo et al, 2012).…”

Section: Relative Symbiotic Effectivenessmentioning

confidence: 99%

Characterization and effectiveness determination of indigenous rhizobia nodulating lablab (lablab purpureus) in Babati district, Tanzania

Malugu

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The soil samples from fifteen villages which grow Lablab in Babati district were collected and used for planting Lablab in experimental pot to trap indigenous rhizobia. At a flowering stage, the plants were harvested and root nodules were collected for rhizobia isolation using Yeast Extract Mannitol Agar (YEMA). Under aseptic conditions, twenty-two isolates were obtained and characterized by morphological, biochemical and physiological characteristics. Furthermore, PCR amplification of 16S rDNA and symbiotic effectiveness were also evaluated. The results showed that, rhizobia were fast and slow growing in YEMA after 4 - 7 days of incubation. In YEMA with Congo red one isolates absorbed the color, while, physiologically all isolates were alkaline tolerant, however susceptible to acidity and sodium chloride above 3%. High diversity among isolates was observed in dendrogram at 70% similarity level by forming two groups with seven clusters. Only 18 isolates out of 22 were amplified. In testing symbiotic effectiveness, all 22 isolates induced nodules in Lablab, interestingly some were effective than commercial inoculants. Most of isolates recorded relative symbiotic effectiveness above 100, the highest was 193 for BR4. Isolates BR4, BR13, BR16, BR5 and BR18 were effective than other isolates and commercial strains. The results implied presence of diverse and effective indigenous rhizobia nodulating Lablab in Tanzania, also highlights their potential to be used for inoculating Lablab instead of commercial inoculants. To recommend characterization by sequencing to identify their genetic groups and field effectiveness evaluation is needed in order to identify more effective isolates for inoculants production.

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Uso da biomanta: Método de substituição de drenagem superficial através da bioengenharia

Borges¹

2020

Revista Científica Multidisciplinar Núcleo do Conhecimento

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Programas de recuperação e proteção de áreas que foram impactadas ambientalmente pela erosão do solo são implementados para corrigir ou prevenir os danos causados, porém nem sempre são adotados métodos sustentáveis. Através do processo governado por agentes climáticos e a intervenção do homem, a erosão têm causado consequências que provocam investigações e debates acerca do assunto, principalmente em âmbito acadêmico e relacionado à bioengenharia. O presente trabalho tem como objetivo dissertar e apresentar de forma documental a temática biomanta, um método com menor impacto ambiental e monetariamente viável. Composta por fibra de coco e polipropileno, a biomanta como uma opção para a substituição de drenagens superficiais, proporciona proteção imediata contra o efeito dos agentes erosivos, conservando a umidade do solo e, consequentemente, trazendo inúmeros benefícios como a proteção da vegetação.

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Symbiotic efficiency of native rhizobia in legume tree Leucaena leucocephala derived from several soil classes of Brazilian Northeast region

Cited by 4 publications

References 21 publications

Plant transcriptome analysis reveals specific molecular interactions between alfalfa and its rhizobial symbionts below the species level

Plant transcriptome analysis reveals specific molecular interactions between alfalfa and its rhizobial symbionts below the species level

Characterization and effectiveness determination of indigenous rhizobia nodulating lablab (lablab purpureus) in Babati district, Tanzania

Uso da biomanta: Método de substituição de drenagem superficial através da bioengenharia

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