Symbiotic Fungus of Marine Sponge Axinella sp. Producing Antibacterial Agent

Trianto, Agus; Widyaningsih, Sekar; Radjasa, O K; Pribadi, Rudhi

doi:10.1088/1755-1315/55/1/012005

Cited by 10 publications

(12 citation statements)

References 30 publications

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“…The preparation of antibacterial activity was initiated by antagonistic testing using the agar plug method (Trianto et al, 2017;Sibero et al, 2018a). The fungus was cultured in MEA for seven days at 27 o C. The mycelium was taken out approximately at the size of a 1-cm diameter circle with a cork borer.…”

Section: Preparation Of Antibacterial Activitymentioning

confidence: 99%

“…Test bacteria were cultured using MHA media with the initial density equivalent to 1.5 x10 8 CFU/ml, and the bioassay was tested using 24-hour-old bacteria. The antibacterial bioassay was conducted by determining the diameter of the inhibition zone using disc diffusion based on Trianto et al (2017). Inhibition zones were measured after a 24-hour incubation period at 37 o C. Extracts in methanol solvent were tested against pathogenic bacteria using discs (Oxoid, 6 mm diameter) with 500 µg/disc concentration.…”

Section: Antibacterial Activitymentioning

confidence: 99%

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Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria

Sedjati

Ambariyanto

Trianto³

et al. 2020

Squalen Bull. Marine Fisheries Postharvest Biotech.

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This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds. Meanwhile, the ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria

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Section: Preparation Of Antibacterial Activitymentioning

confidence: 99%

Section: Antibacterial Activitymentioning

confidence: 99%

Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria

Sedjati

Ambariyanto

Trianto³

et al. 2020

Squalen Bull. Marine Fisheries Postharvest Biotech.

View full text Add to dashboard Cite

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“…Initial antibacterial screening or also called as antagonistic testing was carried out using agar plug method (Trianto et al, 2017;Sibero et al, 2018). Besides, the fungal association was cultured in MEA media for 7 days at 27 0 C temperature.…”

Section: Antimicrobial Activity Screeningmentioning

confidence: 99%

“…The antibacterial bioassay was carried out by determining the diameter of the inhibitory zone using disc diffusion (antibacterial agar disc diffusion assay). It was conducted based on the method used by Trianto et al (2017). Each extract in methanol solvent was tested against pathogenic bacteria using a 6 mm diameter Oxoid disc with 500 μg/disc concentration.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

“…which produce antibacterial E. coli strain MDR (Sibero et al, 2017); Aspergillus sydowii fungus from sponge Axinella sp. produces antibacterial S. aureus and E. coli MDR strains (Trianto et al, 2017) and T. saturnisporum fungus from Pandang Island waters (North Sumatra) produces antibacterial Staphylococcus enterica MDR strain (Sibero et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity of Fungal Extract of The Aspergillus flavus from Hiri Island, North Maluku to Pathogenic Bacteria

et al. 2020

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Antibacterial compounds from sponge association fungi are one of the alternatives to search for new antibiotics against resistant bacteria. This study aims to explore the secondary metabolites potential from sponge association fungi as MDR pathogens antibacterial and to cultivate these fungi using a variety of cultivation media. TE-BO-09.1. Isolate can inhibit 3 bacteria, K. pneumoniae, B subtilis, and S. aureus, but is not able to inhibit E. coli and P. aeruginosa. Cultivation using standard media of Malt Extract Agar (MEA) and media modified from fish broth (M1, M2) and cassava infusion (M3, M4) produce secondary metabolites with varying quantities and antimicrobial activity. The inhibition zone of the produced extract with 500 μg/disc concentration ranged from 7.14 to 10.32 mm. The strongest potential was shown by ethyl acetate extract from isolates cultured with M2 (9 days cultivation), of which is able to produce 10.32 mm inhibition zones against S. aureus and methanol extracts from isolates cultured with M4 (6 days cultivation), and able to produce 10.05 mm inhibition zones against K. pneumoniae. In conclusion, the fungus Aspergillus flavus can be culture using fish broth added glucose and cassava infusion water added peptone media to potentially produce antibacterial compounds against MDR pathogens.

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