The aim of the study was to evaluate arterial stiffness and its modulating factors measured by carotid-femoral pulse wave velocity and central augmentation index in patients with pheochromocytoma before and after surgery. Forty-five patients with pheochromocytoma and 45 healthy controls were investigated using an applanation tonometer (SphygmoCor, AtCor Medical). In addition, 27 patients with pheochromocytoma were studied one year after tumor removal. The gender, age, BMI and lipid profiles were comparable among both groups. The main difference in basic characteristic was as expected fasting plasma glucose (P<0.001) and all blood pressure modalities. Pulse wave velocity in pheochromocytoma was significantly higher than in controls (7.2±1.4 vs.5.8±0.5 m.s-1; P<0.001). Between-group difference in pulse wave velocity remained significant even after the adjustment for age, heart rate, fasting plasma glucose and each of brachial (P<0.001) and 24h blood pressure parameters (P<0.01). The difference in augmentation index between groups did not reach the statistical significance (19±14 vs. 16±13 %; NS). In multiple regression analysis, age (P<0.001), mean blood pressure (P=0.002), high sensitive C-reactive protein (P=0.007) and 24h urine norepinephrine (P=0.007) were independently associated with pulse wave velocity in pheochromocytoma. Successful tumor removal led to a significant decrease in pulse wave velocity (7.0±1.2 vs.6.0±1.1 m.s-1; P<0.001). In conclusion, patients with pheochromocytoma have an increase in pulse wave velocity, which is reversed by the successful tumor removal. Age, mean blood pressure, hs-CRP and norepinephrine levels are independent predictors of pulse wave velocity.