Sympatric species distribution, genetic diversity and population structure of Haemonchus isolates from domestic ruminants in Pakistan

Hussain, Tanveer; Kathiravan, P.; Nadeem, Asif; Babar, Masroor Ellahi; Pichler, Rudolf; Diallo, Adama

doi:10.1016/j.vetpar.2014.10.026

Cited by 37 publications

(41 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings are consistent with other reports from various tropicaldeveloping countries (Nginyi et al, 2001;Mejia et al, 2003;Tan et al, 2014;Hussain et al, 2014). This study show that infections with nematodes was common and especially Strongyles were the most frequent compared with other types of worms.…”

Section: Discussionsupporting

confidence: 93%

Gastrointestinal Parasites of Cattle in Central Java

Hamid

Kristianingrum

Prastowo³

et al. 2016

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

Most of the animals presented monoinfections (79.33%), but concomitant infections were also observed (2 different parasites: 17.67%; 3 different parasites: 2.33%; more than 3 different parasites: 0.67%). With this work we proved the high level of parasitism in cattle of Central Java and we intensively highlight the need to find adequate control measures against parasitic diseases in order to reduce the detrimental impact of parasitosis in cattle.

show abstract

Section: Discussionsupporting

confidence: 93%

Gastrointestinal Parasites of Cattle in Central Java

Hamid

Kristianingrum

Prastowo³

et al. 2016

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…The mean EPG value in the trichostrongylid type positive samples was determined as 120.3±53. 5. EPG values were determined as approximately two times higher in over 2 years age group than under ones and this difference was found statistically significant (P<0.05).…”

Section: Fecal Examination and Real Time Pcr Resultsmentioning

confidence: 70%

“…Genetic characterization of these parasites is also an important indicator for the diagnosis of drug resistance and formation of the control strategies for the parasite infections [6] . In this perspective, the current study evaluated the genetic diversity in H. contortus from sheep in Kayseri region for the first time in Turkey based on the sequences of a mitochondrial DNA marker (mt-COI) and a nuclear ribosomal marker (ITS-2) which have been widely used in phylogenetic characterization of trichostrongylid nematode species [1,4,5] . Genetic diversity among the Kayseri isolates based on the mt-COI gene was determined as higher than ITS-2 gene region which indicates the usefulness of mt-COI gene in the investigation of intraspecific variations and genetic variability in H. contortus populations as also indicated by Hussain et al [5] .…”

Section: Discussionmentioning

confidence: 99%

“…As it has been seen in several nematodes, H. contortus has also a high biotic potential along with a high infection rate and direct life cycle which does not need an intermediate host. These feature gains this parasite a large effective population size with wide genetic variability [5,24] . In accordance with this situation on the characteristics of H. contortus, genetic variability based on mt-COI phylogeny among the Kayseri isolates and the other isolates from Pakistan, USA, Brazil and Australia was also found high with a mean rate of 5.5%.…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose several PCR-based methods targeting mainly the nuclear and mitochondrial genes have been applied and used in the phylogenetic relationships among trichostrongylid nematodes [1,[4][5][6] . However there have been no molecular-based studies conducted on H. contortus populations in Turkey up to date.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Koyunlarda Haemonchus contortus’un Moleküler Prevalansı, Filogenetik Karakterizasyonu ve Benzimidazol Dirençliliği

Önder¹,

Yıldırım²,

İncı³

et al. 2015

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

This study was conducted to determine the molecular prevalence and characterization of Haemonchus contortus from sheep along with the benzimidazole (BZ) resistance in H. contortus populations. Fecal samples were collected from a total of 300 sheep in research area and analyzed by fecal flotation. qPCR assays were utilized on trichostrongylid egg positive samples in order to identify H. contortus. Phylogenetic analyses were performed on ribosomal ITS-2 and mt-COI gene regions. BZ sensitive and resistant allele frequencies were determined by qPCR along with sequence analyses of the β-tubulin isotype 1 gene for single nucleotide polimorphizms (SNPs). H. contortus was identified in 36 (24.8%) out of 145 trichostrongylid egg positive samples. H. contortus isolates showed 100.0% identity to each other and 0.1% difference with the isolates available in the GenBank based on phylogenetic analyses of ITS-2 gene while mt-COI analyses of the isolates exhibited a mean of 97.4±0.5% identity to each other and 5.5±0.8% difference with the isolates in GenBank. BZ sensitive and resistant allele frequencies in H. contortus populations were determined as 87.1%±16.2 and 12.9%±16.2, respectively. SNPs were detected only in the codon 200 of the sequenced isolates belong to resistant allele. This study provides the first data on molecular prevalence, phylogenetic characterization and BZ resistance in H. contortus populations from sheep in Turkey. Keywords

show abstract

Population genetics of benzimidazole-resistant Haemonchus contortus and Haemonchus placei from buffalo and cattle: implications for the emergence and spread of resistance mutations

et al. 2018

View full text Add to dashboard Cite

The population genetics of nematode parasites are poorly understood with practical reference to the selection and spread of anthelmintic resistance mutations. Haemonchus species are important to study the nematode population genetics due to their clinical importance in ruminant livestock, and the availability of genomic resources. In the present study, it has been examined that Haemonchus contortus and Haemonchus placei populations from three buffalo and nine cattle hosts. Seventy-three individual adult worms of H. contortus and 148 of H. placei were analysed using a panel of seven microsatellite markers. The number of alleles per locus in H. contortus and H. placei indicated that all populations were polymorphic for the microsatellites used in the present study. Genetic diversity parameters included high levels of allelic richness and heterozygosity, indicating effective population sizes, high mutation rates and high transmission frequencies in the area. Genetic structure parameters revealed low genetic differentiation between and high levels of genetic variation within H. contortus and H. placei populations. Population dynamic analyses showed an absence of heterozygosity excess in both species, suggesting that there was no deviation from genetic drift equilibrium. Our results provide a proof of concept for better understanding of the consequences of specific control strategies, climatic change or management strategies on the population genetics of anthelmintic resistance alleles in Haemonchus spp. infecting co-managed buffalo and cattle.

show abstract

Sympatric species distribution, genetic diversity and population structure of Haemonchus isolates from domestic ruminants in Pakistan

Cited by 37 publications

References 42 publications

Gastrointestinal Parasites of Cattle in Central Java

Gastrointestinal Parasites of Cattle in Central Java

Koyunlarda Haemonchus contortus’un Moleküler Prevalansı, Filogenetik Karakterizasyonu ve Benzimidazol Dirençliliği

Population genetics of benzimidazole-resistant Haemonchus contortus and Haemonchus placei from buffalo and cattle: implications for the emergence and spread of resistance mutations

Contact Info

Product

Resources

About