Symplasmic Sieve Element Continuity between <i>Orobanche</i> and its Host*

Dörr, Inge; Kollmann, Rainer

doi:10.1111/j.1438-8677.1995.tb00830.x

Cited by 77 publications

(42 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Due to the lack of speciWc cellular markers to distinguish between sieve elements of host and parasite, it was so far not possible to characterise the cellular structures of the phloem connection by electron microscopy. In grafts and in the host-parasite system Vicia narborensis-Orobanche crenata, the interspeciWc plasmodesmata at the interface were developed to open pores Glockmann 1985, 1991;Dörr and Kollmann 1994). In heteroplastic grafts between Cucurbita maxima and Cucumis sativus L. these structures allow an uninhibited phloem transport of the phloem proteins PP1 (MW 96 kDa) and PP2 (MW 48 kDa) from the grafting stock to the scion (Golecki et al 1998).…”

Section: Discussionmentioning

confidence: 98%

Arabidopsis thaliana is a susceptible host plant for the holoparasite Cuscuta spec

Birschwilks

Sauer

Scheel

et al. 2007

Planta

View full text Add to dashboard Cite

Arabidopsis thaliana and Cuscuta spec. represent a compatible host-parasite combination. Cuscuta produces a haustorium that penetrates the host tissue. In early stages of development the searching hyphae on the tip of the haustorial cone are connected to the host tissue by interspecific plasmodesmata. Ten days after infection, translocation of the fluorescent dyes, Texas Red (TR) and 5,6-carboxyfluorescein (CF), demonstrates the existence of a continuous connection between xylem and phloem of the host and parasite. Cuscuta becomes the dominant sink in this host-parasite system. Transgenic Arabidopsis plants expressing genes encoding the green fluorescent protein (GFP; 27 kDa) or a GFP-ubiquitin fusion (36 kDa), respectively, under the companion cell (CC)-specific AtSUC2 promoter were used to monitor the transfer of these proteins from the host sieve elements to those of Cuscuta. Although GFP is transferred unimpedly to the parasite, the GFP-ubiquitin fusion could not be detected in Cuscuta. A translocation of the GFP-ubiquitin fusion protein was found to be restricted to the phloem of the host, although a functional symplastic pathway exists between the host and parasite, as demonstrated by the transport of CF. These results indicate a peripheral size exclusion limit (SEL) between 27 and 36 kDa for the symplastic connections between host and Cuscuta sieve elements. Forty-six accessions of A. thaliana covering the entire range of its genetic diversity, as well as Arabidopsis halleri, were found to be susceptible towards Cuscuta reflexa.

show abstract

Section: Discussionmentioning

confidence: 98%

Arabidopsis thaliana is a susceptible host plant for the holoparasite Cuscuta spec

Birschwilks

Sauer

Scheel

et al. 2007

Planta

View full text Add to dashboard Cite

show abstract

“…Sieve elements were identiWed in Orobanche and Striga asiatica but no obvious phloem-phloem linkages (Kuijt and Toth 1976). In contrast, interspeciWc plasmodesmata were observed in Striga gesneriodes-Pisum associations and between adjacent sieve tubes in Orobanche crenata associations (Dörr 1996;Dörr and Kollman 1995). Extensively invaginated transfer cells have been described that apparently link the host and parasite vasculature in Triphysaria haustoria (Heide-Jorgensen and Kuijt 1993Kuijt , 1995.…”

Section: Introductionmentioning

confidence: 90%

Agrobacterium tumefaciens and Agrobacterium rhizogenes transformed roots of the parasitic plant Triphysaria versicolor retain parasitic competence

Tomilov

Tomilova

Yoder

2006

Planta

View full text Add to dashboard Cite

Parasitic plants in the Orobanchaceae invade roots of neighboring plants to rob them of water and nutrients. Triphysaria is facultative parasite that parasitizes a broad range of plant species including maize and Arabidopsis. In this paper we describe transient and stable transformation systems for Triphysaria versicolor Fischer and C. Meyer. Agrobacterium tumefaciens and Agrobacterium rhizogenes were both able to transiently express a GUS reporter in Triphysaria seedlings following vacuum infiltration. There was a correlation between the length of time seedlings were conditioned in the dark prior to infiltration and the tissue type transformed. In optimized experiments, nearly all of the vacuum infiltrated seedlings transiently expressed GUS activity in some tissue. Calluses that developed from transformed tissues were selected using non-destructive GUS staining and after several rounds of in vivo GUS selection, we recovered uniformly staining GUS calluses from which roots were subsequently induced. The presence and expression of the transgene in Triphysaria was verified using genomic PCR, RT PCR and Southern hybridizations. Transgenic roots were also obtained by inoculating A. rhizogenes into wounded Triphysaria seedlings. Stable transformed roots were identified using GUS staining or fluorescent microscopy following transformation with vectors containing GFP, dsRED or EYFP. Transgenic roots derived from both A. tumefaciens and A. rhizogenes transformations were morphologically normal and developed haustoria that attached to and invaded lettuce roots. Transgenic roots also remained competent to form haustoria in response to purified inducing factors. These transformation systems will allow an in planta assessment of genes predicted to function in plant parasitism.

show abstract

“…The open connections were very rarely observed as in C. odorata-Hibiscus rosa system, in which interconnections via the sieve pores between the sieve tubes of the host and the parasite are found (Schlenzka 1992). Such open direct connections between the sieve elements of the parasite and its host have been described well in the root paratsite Orobanche and its host Vicia system (Dörr and Kollmann 1995).…”

Section: Xylic and Phloic Conductive Hyphaementioning

confidence: 98%

Structure and Development of the Endophyte in the Parasitic Angiosperm Cuscuta japonica

Lee

2009

J. Plant Biol.

View full text Add to dashboard Cite

The endophyte, that is, the haustorial part within the tissues of the host plant Impatiens balsamina, of the parasitic angiosperm Cuscuta japonica was studied with light and electron microscopy. The endophyte consisted mainly of vacuolated parenchymatous axial cells and elongate, superficial (epidermal) cells. Then the elongate, epidermal cells separated from each other and transformed into filamentous cells, called searching hyphae. The hyphae grew independently either intercellularly or intracellularly in the host parenchyma. The apical end of the hyphal cells was characterized by conspicuous, large nuclei with enlarged nucleoli and very dense cytoplasm with abundant organelles, suggesting that the hyphal cells penetrating host tissue were metabolically very active. Numerous osmiophilic particles and chloroplasts were noted in the hyphae. The osmiophilic particles were assumed to be associated with elongation of the growing hyphe. Plasmodemata connections between the searching hyphal cells of the parasite and the host parenchyma cells were not detected. Hyphal cells that reached the host xylem differentiated into water-conducting xylic hyphae by thickening of the secondary walls. A xylem bridge connecting the parasite and the host was confirmed from serial sections. Some hyphal cells that reached the host phloem differentiated into nutrient-conducting phloic hyphae. Phloic hyphae had a thin layer of peripheral cytoplasm with typical features of sieve-tube members in autotrophic angiosperms, i.e., parallel arrays of smooth endoplasmic reticulum, mitochondria, and plastids with starch granules. Interspecific open connections via the sieve pores of the host sieve elements and plasmodesmata of the parasite phloic hyphae were very rarely observed, indicating that the symplastic translocation of assimilate to the parasite from the host occurred.

show abstract

Symplasmic Sieve Element Continuity between Orobanche and its Host*

Cited by 77 publications

References 26 publications

Arabidopsis thaliana is a susceptible host plant for the holoparasite Cuscuta spec

Arabidopsis thaliana is a susceptible host plant for the holoparasite Cuscuta spec

Agrobacterium tumefaciens and Agrobacterium rhizogenes transformed roots of the parasitic plant Triphysaria versicolor retain parasitic competence

Structure and Development of the Endophyte in the Parasitic Angiosperm Cuscuta japonica

Contact Info

Product

Resources

About