Symport H<sup>+</sup>/carbohydrate transport into <i>Acholeplasma laidlawii</i> cells

Tarshis, Mark; Капитанов, А. Б.

doi:10.1016/0014-5793(78)80525-0

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…In Ca2+-treated cells, both drugs were inhibitory for active transport activity. A somewhat unexpected inhibition was observed with another lipid-soluble synthetic ion, TPB-, which nornally has no effect on Aji4+ (37). It is noteworthy that the cell wall of intact E. coli cells is normally not permeable to most of the drugs tested (nigericin, TPP+, valinomycin) but evidently becomes permeable to them upon Ca+2 treatment.…”

Section: Resultsmentioning

confidence: 95%

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Sabelnikov¹,

Domaradsky²

1981

J Bacteriol

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The effect of various metabolic inhibitors (carbonylcyanid-m-chlorophenylhydrazone, nigericin, valinomycin, dicyclocarbodliimide, arsenate, NaF, etc.) and lipid-soluble synthetic ions (tetraphenylphosphonium bromide and tetraphenylboron sodium) on deoxyribonucleic acid (DNA) entry during transformation of Ca2+-treated Escherichia coli cells with plasmid DNA and on cell viability was investigated. In contrast to intact cells, Ca2+-treated E. coli cells were permeable to nigericin, valinomycin, and the other drugs tested. The inhibitors differentially affected [14C]proline active transport, and whereas some drugs inhibited transformation, the effects did not correlate with the effects on transport. The most potent inhibitors of transformation were nigericin, dicyclocarbodiimide, and tetraphenylboron sodium. Carbonylcyanid-m-chlorophenylhydrazone, tetraphenylphosphonium bromide, and valinomycin were relatively inactive. Tetraphenylboron sodium-and nigericin-treated cells bound more plasmid [14C]DNA in the deoxyribonuclease-resistant form than the control and other sample cells. Nevertheless, the penetration of exogenous plasmid DNA into the cell was greatly reduced, at least in case of nigericin. Unlike the other drugs, nigericin and dicyclocarbodiimide drastically affected the cell viability, the former within very short times of interaction. It is concluded that proton motive force does not play any significant role in DNA entry into Ca2+-treated E. coli cells. The results also suggest that adenosine 5'-triphosphate is not required for DNA entry either. The inhibitory effect of certain drugs is discussed in terms of structural perturbations induced by the drugs in cell envelope membranes. the presence of the uncoupler CCCP, Ca2+treated cells do remain permeable to exogenous DNAs and some DNA-binding antibiotics, even at 370C, whereas nornally they lose permeability. These data, although suggestive, cannot give an unambiguous answer as to the nature of the energy requirement during exogenous DNA up-435

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Section: Resultsmentioning

confidence: 95%

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Sabelnikov¹,

Domaradsky²

1981

J Bacteriol

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“…According to LeBlanc and LeGrimellec (16), treatment of M. gallisepticum cells with the combination of CCCP and valinomycin in a K+-free buffer should have dissipated the cell membrane potential; in our experiments, however, it only slightly reduced the hyperpolarization effect which was induced by valinomycin alone. This probably means that the efflux of K+ ions induced by valinomycin (26) is faster under these conditions than the dissipation of the H+ gradient by CCCP (16).…”

Section: Discussionmentioning

confidence: 98%

“…Several treatments were employed to manipulate the energized state of M. gallisepticum cells, including incubation in buffer depleted of energy sources (7,11,16), inhibition of metabolic pathways (7,12,14), and blocking the Mg2+-ATPase activity with DCCD (6,16,22,26). LeBlanc and LeGrimellec (16) obtained energydepleted Mycoplasma mycoides subsp.…”

Section: Discussionmentioning

confidence: 99%

Effects of ionophores and dicyclohexylcarbodiimide on Mycoplasma gallisepticum adherence to erythrocytes

Banai¹,

Razin²,

Schuldiner³

et al. 1982

Infect Immun

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To test the influence of the electrochemical ion gradient across mycoplasma membranes on the capacity of organisms to adhere to host cells, Mycoplasma gallisepticum cells were treated with valinomycin, carbonylcyanide m-chlorophenylhydrazone, and N,N'-dicyclohexylcarbodiimide (DCCD) singly or in combination. Uptake of [3H]tetraphenylphosphonium by the treated cells was employed as a measure of the effects of the ionophores on membrane potential. In the absence of K+, valinomycin increased, whereas carbonylcyanide m-chlorophenylhydrazone, and DCCD decreased [3H]tetraphenylphosphonium uptake. However, with a high level of K+ or with DCCD, uptake of [3H]tetraphenylphosphonium in the presence of valinomycin decreased below control levels, indicating that, generally, the ionophores affected membrane potential in the expected manner. The treated organisms were tested for their capacity to attach to glutaraldehyde-fixed human erythrocytes. DCCD was the best inhibitor of mycoplasma attachment, and in combination with valinomycin attachment, capacity decreased by about 40%. The combination of valinomycin plus carbonylcyanide m-chlorophenylhydrazone was less effective; it decreased attachment by about 15 to 25%. It was concluded that the dissipation of ion gradients across cell membranes decreases only partially mycoplasma adherence, in line with previous findings that isolated mycoplasma membranes retain the major part of the attachment capacity of intact cells.

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“…reaction. Dicyclohexylcarbodiimide (DCCD) which inactivates mycoplasma proton-translocating F1-F0-ATPase [20], and carbonyl cyanide mchlorophenylhydrazone (CCCP) which suppresses mycoplasma transmembrane proton gradient [21], were ineffective. A slight enhancement of activity occurred with pronase-treated mycoplasmas, indicating the involvement of membrane proteasesensitive sites in plasminogen activation.…”

Section: Factors Influencing Mycoplasma-dependent Plasminogen Activationmentioning

confidence: 99%

Mycoplasma cells stimulate in vitro activation of plasminogen by purified tissue-type plasminogen activator

Tarshis

Morag

Mayer

1993

FEMS Microbiology Letters

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In an in vitro direct assay with tissue-type plasminogen activator (tPA), plasminogen and the chromogenic substrate S-2251, the ability of Mycoplasma fermentans KL4 to stimulate tPA-mediated activation of plasminogen to plasmin was studied. Mycoplasma cells markedly enhanced the activation of plasminogen by tPA in a concentration-, temperature- and pH-dependent manner. Nonidet P-40 (0.01%), sonication, and freezing and thawing of the cells substantially increased the stimulatory effect of mycoplasma on tPA activity. In contrast, the activation of plasminogen by urokinase was refractory to mycoplasma cells. The mycoplasma-mediated stimulation of tPA activity was prevented by epsilon-aminocaproic acid (EACA), a lysine analogue known to block lysine-binding sites (LBS) in plasminogen and tPA. Among several Mycoplasma fermentans strains tested, incognitus strain demonstrated the highest stimulation activity. These results suggest that mycoplasma cells interact with LBS in tPA and plasminogen to enhance plasminogen activation.

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Symport H⁺/carbohydrate transport into Acholeplasma laidlawii cells

Cited by 8 publications

References 18 publications

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Effects of ionophores and dicyclohexylcarbodiimide on Mycoplasma gallisepticum adherence to erythrocytes

Mycoplasma cells stimulate in vitro activation of plasminogen by purified tissue-type plasminogen activator

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Symport H+/carbohydrate transport into Acholeplasma laidlawii cells

Cited by 8 publications

References 18 publications

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Effects of ionophores and dicyclohexylcarbodiimide on Mycoplasma gallisepticum adherence to erythrocytes

Mycoplasma cells stimulate in vitro activation of plasminogen by purified tissue-type plasminogen activator

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Symport H⁺/carbohydrate transport into Acholeplasma laidlawii cells