Symposium on the Maintenance of Cultures of Microorganisms1

Perlman, D.; Hartsell, S. E.; Haskins, R. H.; Blackwood, A. C.; Haynes, W. C.; Wickerham, Lynferd J.; Hesseltine, C. W.; Heckly, Robert J.; Klumpp, Melvin N.; Hutner, S. H.; Provasoli, Luigi; Skeggs, Helen R.

doi:10.1128/mmbr.19.4.280-283.1955

Bacteriological Reviews

1955

DOI: 10.1128/mmbr.19.4.280-283.1955

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Symposium on the Maintenance of Cultures of Microorganisms1

D. Perlman¹,

S. E. Hartsell²,

R. H. Haskins³

et al.

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“…Further information concerning this method of preserving bacteria may be found in the report of the symposium held in New Pork in May, 1955, on 'Maintenance of Cultures of Micro-organisms' (Perlman et al, 1955;Hartsell, 1956). From the available evidence the mineral oil technique appeared effective and reliable for the preservation of many differint kinds of micro-organisms ; Dr. Hartsell recomrnendcd the method on the grounds of ease and simplicity.…”

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THE PRESERVATION OF PSEUDOMONAS UNDER MINERAL OIL

Rhodes

1957

Journal of Applied Bacteriology

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The use of mineral oil for the preservation of species of Pseudomonas is described. Eighty-three out of 91 (91.2%) PsewEornonas isolates remained viable for at least 66 months; 156 out of 168 (92.8%) isolates were still living after at least 46 months, and only 12 out of 170 (2.3%) isolates were non-viable after three years. An explanation is offered for variation in ability to grow and produce acid in chemically defined media containing galactose or sucrose. The ability to hydrolyze tributyrin and to reduce nitrate was stable over 66 months, regardless of whether the stock cultures were maintained under mineral oil or in yeast-extract peptone broth. However, the ability to digest milk or gelatin was frequently impaired or lost when cultures were maintained in peptone broth, although the mineral oil-preserved stock cultures remained stable in this respect. Over a nineteen month period six diagnostic biochemical characters of the oil-covered stock cultures remained stable within the limits of the experimental methods used. Data from the literature and information from other workers reveal that more complex methods of preserving pseudomonads are not particularly advantageous in regard either to viability or stability. The mechanism of the survival of bacteria under mineral oil is discussed. The taxonomic significance of the loss of proteolytic activity by the 'bench-broth' cultures of Pseudomonas spp. is illustrated by a single example. PROOM, H. & HEMMONS. L. M. (1949). The drying and preservation of bacterial cultures. J. sen. Microbiol. 3, 7. RHODES, MABEL (1950). Viability of dried bacterial cultures. J . gen. itficrobio~. 4, 450. RHODES, M. E. (1956). The classification of Pseudomom. Ph.D. thesis, University of Reading, SEER+, A. F. (1943). A method for maintaining Corynebacterium sepedonicum in culture for long May 1956. periods without transfer. Phyhpthology 33, 330.

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THE PRESERVATION OF PSEUDOMONAS UNDER MINERAL OIL

Rhodes

1957

Journal of Applied Bacteriology

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Erfahrungen mit Streptomyceten-Dauerkulturen

Frommer

1956

Archiv. Mikrobiol.

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Symposium on the Maintenance of Cultures of Microorganisms1

Cited by 2 publications

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THE PRESERVATION OF PSEUDOMONAS UNDER MINERAL OIL

THE PRESERVATION OF PSEUDOMONAS UNDER MINERAL OIL

Erfahrungen mit Streptomyceten-Dauerkulturen

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