Symptomatic atherosclerosis is associated with an altered gut metagenome

Karlsson, Fredrik; Fåk, Frida; Nookaew, Intawat; Tremaroli, Valentina; Fagerberg, Björn; Petranović, Dina; Bäckhed, Fredrik; Nielsen, Jens

doi:10.1038/ncomms2266

Cited by 1,083 publications

(904 citation statements)

References 46 publications

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“…This constellation seems to be a specific attribute of heart failure, as there is no other disease entity reported to be accompanied by this alteration. It is conceivable that depletion of these genera contributes to HF pathogenesis, as recent findings from other inflammatory diseases suggest: For example, Collinsella bacteria have been reported to be associated with systemic atherosclerosis and type 2 diabetes mellitus (T2DM) 34, 35. Interestingly, this genus seems to be enriched in patients suffering from atherosclerosis or T2DM, whereas we could demonstrate its depletion in HF patients.…”

Section: Discussionmentioning

confidence: 99%

Heart failure is associated with depletion of core intestinal microbiota

Luedde¹,

et al. 2017

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AimsIn spite of current medical treatment approaches, mortality of chronic heart failure (HF) remains high and novel treatment modalities are thus urgently needed. A recent theory proposes a possible impact of the intestinal microbiome on the incidence and clinical course of heart failure. This study sought to systematically investigate, if there are specific changes of the intestinal microbiome in heart failure patients.Methods and resultsThe intestinal microbiome of 20 patients with heart failure with reduced ejection fraction due to ischemic or dilated cardiomyopathy was investigated by applying high‐throughput sequencing of the bacterial 16S rRNA gene. Microbial profiles were compared to those of matched controls in which heart failure was ruled out by clinical assessment and NT‐proBNP serum levels (n = 20). According to the Shannon diversity index (which measures the intra‐individual alpha‐diversity) based on the distribution of operational taxonomic units (OTUs), HF cases showed a nominally significantly lower diversity index compared to controls (P nom. = 0.01), and testing for genera abundance showed a tendency towards a decreased alpha diversity of HF patients. Beta‐diversity measures (inter‐individual diversity) revealed a highly significant separation of HF cases and controls, (e.g. P weighted UniFracv = 0.004). Assessing the individual abundance of core measurable microbiota (CMM), a significant decrease of Coriobacteriaceae, Erysipelotrichaceae and Ruminococcaceae was observed on the family level. In line with that, Blautia, Collinsella, uncl. Erysipelotrichaceae and uncl. Ruminococcaceae showed a significant decrease in HF cases compared to controls on the genus level.ConclusionsHeart failure patients showed a significantly decreased diversity of the intestinal microbiome as well as a downregulation of key intestinal bacterial groups. Our data point to an altered intestinal microbiome as a potential player in the pathogenesis and progression of heart failure.

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Section: Discussionmentioning

confidence: 99%

Heart failure is associated with depletion of core intestinal microbiota

Luedde¹,

et al. 2017

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“…negatively affecting the health status of the host, such as malnutrition, colorectal cancer, obesity, diabetes, and cardiovascular disease, many of which are metabolic diseases [5,20,40,46,52]. This association between gut flora and diseases has aided in our understanding of the importance of gut microbiota in the health of the host.…”

Section: Introductionmentioning

confidence: 99%

Fecal Bacterial Composition of the Endangered Yangtze Finless Porpoises Living Under Captive and Semi-natural Conditions

et al. 2015

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Intestinal microbiota is essential to the health and physiology of host animals. We undertook the first microbiological study of the fecal bacterial composition from critically endangered (CR) Yangtze finless porpoises (Neophocaena asiaeorientalis asiaeorientalis; YFPs) living under captive and semi-natural conditions using both high-throughput sequencing method and 16S rRNA gene clone library method. As determined by high-throughput sequencing of V3-V4 regions of the 16S rRNA gene, seminatural samples harbored 30 and 36 operational taxonomic units (OTUs), which was more than the 22 and 27 OTUs detected from YFPs living in captivity. In captive YFPs Firmicutes was the predominant phylum, whereas this was Proteobacteria for YFPs living in semi-nature conditions. This suggests habitat-specific fecal bacterial composition of YFPs. Plesiomonas spp. and Aeromonas spp., which are potentially pathogenic, were identified in all the feces. Bacterial diversity from one porpoise living in captivity was also determined by constructing a 16S rRNA gene clone library and only 1 phylum was identified. Highthroughput sequencing was more effective at determining the bacterial diversity compared to the 16S rRNA gene clone library. This study provides important information for the management and conservation of the CR YFPs.

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“…the development of atherosclerosis have been reported (7)(8)(9). The important concept of the role of gut microbiota in insulin resistance was first described by Cani and colleagues (4,(10)(11)(12)(13).…”

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confidence: 99%

Gut Dysbiosis and Detection of “Live Gut Bacteria” in Blood of Japanese Patients With Type 2 Diabetes

et al. 2014

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OBJECTIVEMounting evidence indicates that the gut microbiota are an important modifier of obesity and diabetes. However, so far there is no information on gut microbiota and "live gut bacteria" in the systemic circulation of Japanese patients with type 2 diabetes. RESEARCH DESIGN AND METHODSUsing a sensitive reverse transcription-quantitative PCR (RT-qPCR) method, we determined the composition of fecal gut microbiota in 50 Japanese patients with type 2 diabetes and 50 control subjects, and its association with various clinical parameters, including inflammatory markers. We also analyzed the presence of gut bacteria in blood samples. RESULTSThe counts of the Clostridium coccoides group, Atopobium cluster, and Prevotella (obligate anaerobes) were significantly lower (P < 0.05), while the counts of total Lactobacillus (facultative anaerobes) were significantly higher (P < 0.05) in fecal samples of diabetic patients than in those of control subjects. Especially, the counts of Lactobacillus reuteri and Lactobacillus plantarum subgroups were significantly higher (P < 0.05). Gut bacteria were detected in blood at a significantly higher rate in diabetic patients than in control subjects (28% vs. 4%, P < 0.01), and most of these bacteria were Gram-positive.

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Symptomatic atherosclerosis is associated with an altered gut metagenome

Cited by 1,083 publications

References 46 publications

Heart failure is associated with depletion of core intestinal microbiota

Heart failure is associated with depletion of core intestinal microbiota

Fecal Bacterial Composition of the Endangered Yangtze Finless Porpoises Living Under Captive and Semi-natural Conditions

Gut Dysbiosis and Detection of “Live Gut Bacteria” in Blood of Japanese Patients With Type 2 Diabetes

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