Symptomatic Management of Postoperative Bullous Keratopathy With Nonpreserved Human Amniotic Membrane

Mejía, Luis Fernando; Santamaría, Juan P.; Acosta, Claudia

doi:10.1097/00003226-200205000-00002

Cited by 60 publications

(47 citation statements)

References 37 publications

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“…Defects in the corneal epithelium requiring surgical reconstruction may result, for example, from ulcers [1][2][3][4][5][6][7] , bullous [8,9] or band [10] keratopathy, and thermal and chemical burns [11,12] . Large defects in the conjunctiva carry the risk of scarring that can disfi gure the conjunctiva and even restrict ocular motility.…”

Section: Introductionmentioning

confidence: 99%

Biomechanical Characterization of Human Amniotic Membrane Preparations for Ocular Surface Reconstruction

et al. 2004

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Purpose: To investigate the tensile and elastic properties of both commercially available and experimental human amniotic membrane preparations. Method: Nine preparations of human amniotic membrane were studied. The four dry preparations were untreated (nonirradiated, n = 20), and gamma (n = 25), low-dose (AmbioDry^®, Okto Ophtho Inc., Costa Mesa, Calif., USA, n = 20) and high-dose (n = 20) electron beam sterilized. The same dry membranes were moistened with balanced salt solution (n = 20, 34, 20 and 20, respectively). The ninth group consisted of thawed medium-frozen amniotic membrane (AmnioGraft^®, Bio-Tissue Inc., Miami, Fla., USA, n = 20). The membranes were cut into thin strips, loaded on a gram range load sensor, and stretched incrementally to the point of rupture. The modulus of elasticity, displacement until rupture and maximum tolerated stress were recorded and compared. Results: The dry preparations exhibited higher moduli of elasticity when compared with the moist samples, with the low-dose electron beam-irradiated samples having the greatest mean modulus of elasticity overall and maintaining a high modulus of elasticity as a moist sample (p < 0.05). Moist nonirradiated preparations and thawed medium-frozen preparations stretched the farthest before rupture and experienced the greatest mean stresses at the point of rupture. While 3 of 4 membranes had greater stretch when moistened as compared to their dry counterparts, there was no difference in the membrane stiffness between dry and moistened low-dose electron beam-irradiated samples (p > 0.8). Conclusions: Low-dose electron beam-irradiated amnion appeared to maintain desirable elastic characteristics in transition from a dry to rehydrated state and may thus provide an easy-to-manipulate transplant tissue for ocular surface reconstruction. Moist nonirradiated and thawed medium-frozen tissues, however, may provide surgical advantages as they required greater forces to rupture.

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Section: Introductionmentioning

confidence: 99%

Biomechanical Characterization of Human Amniotic Membrane Preparations for Ocular Surface Reconstruction

et al. 2004

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“…Symptoms of PBK resolved completely in 8 (88%) patients and partially in 1 patient. 18 Anterior stromal puncture involves multiple micro punctures. The subsequent healing response leads to alteration of extracellular matrix by increasing the expression of fibronectin, laminin and collagen IV at the puncture sites.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study to Evaluate The Role of Amniotic Membrane Transplant and Anterior Stromal Puncture in Painful Pseudophakic Bullous Keratopathy

Chugh¹

2017

Delhi Journal of Ophthalmology

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“…Dual et al (2004) and Mejia et al (2002) reported that both preserved and non-preserved amniotic membrane can be stored. Dimethyl sulfoxide (DMSO) has been used as an alternative to the preservation of amniotic membrane by replacing the rinsing with saline and antibiotics; AzuaraBlanco et al (1999) used DMSO to 4, 8 and 10, while Kubo et al (2001) used the same product to 0.5, 1.0 and 1.5 M for the washing membrane.…”

Section: Preparation and Preservationmentioning

confidence: 99%

“…Dimethyl sulfoxide (DMSO) has been used as an alternative to the preservation of amniotic membrane by replacing the rinsing with saline and antibiotics; AzuaraBlanco et al (1999) used DMSO to 4, 8 and 10, while Kubo et al (2001) used the same product to 0.5, 1.0 and 1.5 M for the washing membrane. After such treatment, it may be stored in 1.5 M DMSO to −80˚C for several months [11,19,26,54].…”

Section: Preparation and Preservationmentioning

confidence: 99%

Amniotic membrane as a potent source of stem cells and a matrix for engineering heart tissue

Francisco¹,

Cunha²,

Simeoni³

et al. 2013

JBiSE

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Existing therapies for the treatment of chronic heart failure still have some limitations and there is a pressing need for the development of new therapeutic modalities. The amniotic membrane has been used for the treatment of various diseases, such as conjunctive defects; however, the mechanisms behind its repair functions are still unclear. Regenerative medicine is seeking newer alternatives and among them, biomaterials have emerged in recent years for developing and manipulating molecules, cells, tissues or organs grown in laboratories in order to replace human body parts. Many such materials have been used for this purpose, either synthetically or biologically, in order to provide new medical devices. This review provides a wider view of the regeneration potential of the use of amniotic membrane as a potential biomaterial to facilitate the implementation of new research in surgical procedures. Amniotic membrane appears to be an alternative source of stem cells as well as an excellent biomaterial for cell-based therapeutic applications in engineering heart tissue.

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Symptomatic Management of Postoperative Bullous Keratopathy With Nonpreserved Human Amniotic Membrane

Abstract: NP-AMT is a good alternative for the management of painful PBK in eyes with poor visual potential; NP-AMT is widely available, the technique is easy to perform, and it has good results from both the symptomatic and esthetic standpoint.

Cited by 60 publications

References 37 publications

Biomechanical Characterization of Human Amniotic Membrane Preparations for Ocular Surface Reconstruction

Biomechanical Characterization of Human Amniotic Membrane Preparations for Ocular Surface Reconstruction

A Comparative Study to Evaluate The Role of Amniotic Membrane Transplant and Anterior Stromal Puncture in Painful Pseudophakic Bullous Keratopathy

Amniotic membrane as a potent source of stem cells and a matrix for engineering heart tissue

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