Symptoms of Plant Virus Infection

Stevens, W. A.

doi:10.1007/978-1-4757-1251-3_2

Cited by 1 publication

(2 citation statements)

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“…These structures may contain virus particles, virusrelated materials, or ordinary cell constituents in a normal or degenerate condition, either singly or, more often, in various proportions (Martelli & Russo, 1977). Inclusion bodies differ in form from amorphous structures to well-defined crystalline structures; some of them are large enough to be seen under light microscopy, while others can only be detected under electron microscopy (Stevens, 1983). The paracrystalline, fibrous, spindle-shaped inclusions are composed of linear aggregates of virus particles united end-to-end (Esau, 1967).…”

Section: Discussionmentioning

confidence: 99%

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Pathological Changes in Sweet Basil (Ocimum basilicum L.) Caused by Mixed Infection of Tobacco Mosaic Virus and Phytoplasma

et al. 2021

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Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 59 O CIMUM basilicum L. (a medicinal plant known as sweet basil) was investigated and showed disease symptoms, including mosaic mottling, leaf distortion, blistering, witches' broom, and phyllody. The plants were found to be infected by both tobacco mosaic virus (TMV) and phytoplasma. Positive TMV was indicated by serological identification using direct antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Phytoplasma infection was identified molecularly using the phytoplasma P1/P7 primer pair and the nested primer, R16F2n/R16R2, which successfully generated a DNA band at ~1200 bp. The virus was successfully transmitted mechanically to Nicotiana tabacum, Datura metal, Datura stramonium, and Phaseolus vulgaris. Light and transmission electron microscopy showed pleomorphic phytoplasmas and rod-shaped particles (300 nm) of TMV, respectively. Histopathological and cytopathological studies revealed changes in the cell behavior of the infected leaves of sweet basil due to virus and phytoplasma infection. Hypoplasia and hyperplasia were observed in the mosaic areas of the mesophyll. Alterations in the vascular tissues and degeneration of the organelles were observed in ultrathin sections of the infected mesophyll. The presence of phytoplasma cells and crystalline bodies were indicative of the mixed infection. To our knowledge, this is the first report of a mixed infection of TMV and phytoplasmas in sweet basil.

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Section: Discussionmentioning

confidence: 99%

“…During multiplication of the causal agent in cells, certain pathological phenomena may occur, such as necrosis or hypoplasia (Schneider, 1973). Furthermore, viruses can disturb the internal organization of cells by altering their form, appearance, and arrangement (Stevens, 1983).…”

Section: Introductionmentioning

confidence: 99%