Synaptic circuitry in the retinorecipient layers of the optic tectum of the lamprey (Lampetra fluviatilis). A combined hodological, GABA and glutamate immunocytochemical study

Repérant, J; Ward, Roger; Médina, Monique; Кенигфест, Н. Б.; Rio, J.P.; Miceli, D.; Jay, Bruno

doi:10.1007/s00429-009-0205-9

Cited by 2 publications

(1 citation statement)

References 134 publications

(281 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Physiological studies in adult lampreys indicate that a number of synapses of tectoreticular fibers use excitatory amino acid neurotransmission (Zompa and Dubuc,1998), which can be related to the observed abundance of glutamatergic tectal cells (present results). Our results are also consistent with the observation of glutamate immunoreactivity in fibers of the optic tract and the retinorecipient region of the optic tectum (Repérant et al,2009). As regards VGLUT expression, the distribution of positive cells in larvae and adults differ, the former exhibiting the most positive cells arranged in compact radial cell columns whereas, glutamatergic cells show a rather loosely layered arrangement in adult.…”

Section: Discussionsupporting

confidence: 93%

Glutamatergic neuronal populations in the brainstem of the sea lamprey, Petromyzon marinus: An in situ hybridization and immunocytochemical study

Villar-Cerviño

Barreiro‐Iglesias

Fernández-López

et al. 2012

J of Comparative Neurology

View full text Add to dashboard Cite

Glutamate is the major excitatory neurotransmitter in vertebrates, and glutamatergic cells probably represent a majority of neurons in the brain. Physiological studies have demonstrated a wide presence of excitatory (glutamatergic) neurons in lampreys. The present in situ hybridization study with probes for the lamprey vesicular glutamate transporter (VGLUT) provides an anatomical basis for the general distribution and precise localization of glutamatergic neurons in the sea lamprey brainstem. Most glutamatergic neurons were found within the periventricular gray layer throughout the brainstem, with the following regions being of particular interest: the optic tectum, torus semicircularis, isthmus, dorsal and medial nuclei of the octavolateral area, dorsal column nucleus, solitary tract nucleus, motoneurons, and reticular formation. The reticular population revealed a high degree of cellular heterogeneity including small, medium-sized, large, and giant glutamatergic neurons. We also combined glutamate immunohistochemistry with neuronal tract-tracing methods or γ-aminobutyric acid (GABA) immunohistochemistry to better characterize the glutamatergic populations. Injection of Neurobiotin into the spinal cord revealed that retrogradely labeled small and medium-sized cells of some reticulospinal-projecting groups were often glutamate-immunoreactive, mostly in the hindbrain. In contrast, the large and giant glutamatergic reticulospinal perikarya mostly lacked glutamate immunoreactivity. These results indicate that glutamate immunoreactivity did not reveal the entire set of glutamatergic populations. Some spinal-projecting octaval populations lacked both VGLUT and glutamate. As regards GABA and glutamate, their distribution was largely complementary, but colocalization of glutamate and GABA was observed in some small neurons, suggesting that glutamate immunohistochemistry might also detect non-glutamatergic cells or neurons that co-release both GABA and glutamate.

show abstract

Section: Discussionsupporting

confidence: 93%