Synaptic interactions regulate gephyrin expression in avian cholinergic neurons in vivo

Allaire, Pauline; Ikonomov, Ognian C.; Garrett, Marianne Kulesa; Jacob, Michele H.

doi:10.1016/s0028-3908(00)00154-4

Cited by 2 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, we observed a higher incidence of VAChT+ terminals associated with Geph+ profiles (∼30%), and one which far exceeded the colocalization of VAChT and VGAT in BF terminals (above). As examined in avian peripheral ganglia (Allaire et al ., 2000), Geph is not known to be associated with or play a role in the assembly of any known ACh receptor type. Most significantly, however, ChAT‐immunostained terminals in the cortex have been found to form junctions primarily of the symmetric type (Houser et al ., 1985; Umbriaco et al ., 1994; Turrini et al ., 2001), which are commonly associated with Geph (Sassoe‐Pognetto & Fritschy, 2000).…”

Section: Discussionmentioning

confidence: 99%

Projections from basal forebrain to prefrontal cortex comprise cholinergic, GABAergic and glutamatergic inputs to pyramidal cells or interneurons

Henny

Jones

2008

Eur J of Neuroscience

246

229

View full text Add to dashboard Cite

The present study was undertaken to characterize the pre-and postsynaptic constituents of the basal forebrain (BF) projection to the prefrontal cortex in the rat, and determine whether it includes glutamatergic in addition to established γ-aminobutyric acid (GABA)ergic and cholinergic elements. BF fibres were labelled by anterograde transport using biotin dextran amine (BDA) and dual-stained for the vesicular transporter proteins (VTPs) for glutamate (VGluT), GABA (VGAT) or acetylcholine (VAChT). Viewed by fluorescence microscopy and estimated by stereology, proportions of BDAlabelled varicosities were found to be stained for VGluT2 (and not VGluT1 or 3), VGAT or VAChT (representing, respectively, ~15%, ~52% and ~19% within the infralimbic cortex). Each type was present in all, though commonly most densely in deep, cortical layers. Material was triple-stained for postsynaptic proteins to examine whether BDA+VTP+ varicosities might form excitatory or inhibitory synapses, respectively, labelled by postsynaptic density-95 kDA (PSD-95) or gephyrin (Geph). Viewed by confocal microscopy, a majority of BDA+/VGluT2+ varicosities were found to be apposed to PSD-95+ elements, and a majority of BDA+/VGAT+ varicosities to be apposed to Geph+ elements. Other series were triple-stained for cell marker proteins to assess whether the varicosities contacted interneurons or pyramidal cells. Viewed by confocal microscopy, BDAlabelled VGluT2+, VGAT+ and VAChT+ BF terminals were all found in contact with calbindin+ interneurons, whereas VGAT+ BF terminals were also seen in contact with parvalbumin+ interneurons and non-phosphorylated neurofilament+ pyramidal cells. Through distinct glutamatergic, GABAergic and cholinergic projections, the BF can thus influence cortical activity in a diverse manner.

show abstract