Synaptic Vesicle Protein SV2B, but Not SV2A, Is Predominantly Expressed and Associated with Microvesicles in Rat Pinealocytes

Hayashi, Mitsuko; Yamamoto, Akira; Yatsushiro, Shouki; Yamada, Hiroshi; Futai, Masamitsu; Yamaguchi, Akihito; Moriyama, Yoshinori

doi:10.1046/j.1471-4159.1998.71010356.x

Cited by 32 publications

(26 citation statements)

References 33 publications

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“…The high sequence homology and the localization of these two isoforms with secretory granules points to their functional importance in hormone secretion. Like in the neuroendocrine pinealocytes (Hayashi et al, 1998), SV2B is only present on SLM in INS-1E cells.…”

Section: Discussionmentioning

confidence: 96%

“…The three SV2 isoforms are also differentially distributed in the mouse retina (Wang et al, 2003). Regarding the neuroendocrine cells, SV2A is detected in the cell line PC12 whereas SV2B is associated with microvesicles of rat pinealocytes (Bajjalieh et al, 1994;Hayashi et al, 1998). In adrenal chromaffin cells, SV2A is mainly localized to granules whereas SV2C is enriched on microsomes .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

SV2A and SV2C are not vesicular Ca2+ transporters but control glucose-evoked granule recruitment

Iezzi¹,

Theander²,

Janz

et al. 2005

Journal of Cell Science

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

SV2A and SV2C are not vesicular Ca2+ transporters but control glucose-evoked granule recruitment

Iezzi¹,

Theander²,

Janz

et al. 2005

Journal of Cell Science

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“…Because Rab proteins confer specificity to vesicles, these results indicate that "synaptic vesicle-like vesicles" function in trafficking and secretion in general tissues. Some recent studies reported that SV2B plays an important role in maintaining tissue-specific function (15)(16)(17)(18). The tissue-specific expression of SV2B suggests that SV2B plays a role in forming the tissuespecific structure.…”

Section: Discussionmentioning

confidence: 99%

Synaptic Vesicle Protein 2B Is Expressed in Podocyte, and Its Expression Is Altered in Proteinuric Glomeruli

Miyauchi

Saito²,

Karasawa³

et al. 2006

Journal of the American Society of Nephrology

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Synaptic vesicle protein 2B (SV2B) was identified by the subtraction hybridization technique as a molecule of which mRNA expression was decreased in puromycin aminonucleoside (PAN) nephropathy by glomerular cDNA subtraction assay. The expression of SV2B was detected in glomerular lysate with Western blot analysis. Dual-labeling immunofluorescence studies with glomerular cell markers demonstrated that SV2B is expressed in glomerular visceral epithelial cells (podocytes). The expression of SV2B is detected also in cultured podocyte and in human kidney section as podocytic pattern. The decrease of SV2B mRNA was already detected before the onset of proteinuria in PAN nephropathy. The mRNA expression of SV2B clearly is altered not only in PAN nephropathy but also in another proteinuric state that is caused by an antibody against nephrin, a functional molecule of the slit diaphragm. The decreased intensity in SV2B staining was already detected before the peak of proteinuria in both models with immunofluorescence study. A reduced amount of SV2B was detected in both models also with Western blot analysis. CD2AP, another functional molecule of the slit diaphragm, was observed in cytoplasm, including the processes area of the cultured podocyte, and when the podocyte was treated with small interfering RNA for SV2B, CD2AP staining at the process area was not detected. These results suggest that SV2B is a functional molecule of podocyte, and SV2B may play a role in the expression and proper localization of CD2AP.

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“…SV2 was understood to be exclusively expressed in the neuronal tissue. However, some reports have shown that SV2s are expressed in several other tissues [51,52] . SV2B is detected in the microvesicles of pinealocytes.…”

Section: Sv2bmentioning

confidence: 99%

“…SV2B is detected in the microvesicles of pinealocytes. Both pinealocyte and podocyte are characterized by their specialized processes [52] . These properties also suggested that SV2B plays a role in the trafficking to the terminal of processes.…”

Section: Sv2bmentioning

confidence: 99%

Therapeutic target for nephrotic syndrome: Identification of novel slit diaphragm associated molecules

Fukusumi¹

2014

WJN

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The slit diaphragm bridging the neighboring foot processes functions as a final barrier of glomerular capillary wall for preventing the leak of plasma proteins into primary urine. It is now accepted that the dysfunction of the sit diaphragm contributes to the development of proteinuria in several glomerular diseases. Nephrin, a gene product of NPHS1 , a gene for a congenital nephrotic syndrome of Finnish type, constitutes an extracellular domain of the slit diaphragm. Podocin was identified as a gene product of NPHS2 , a gene for a familial steroid-resistant nephrotic syndrome of French. Podocin binds the cytoplasmic domain of nephrin. After then, CD2 associated protein, NEPH1 and transient receptor potential-6 were also found as crucial molecules of the slit diaphragm. In order to explore other novel molecules contributing to the development of proteinuria, we performed a subtraction hybridization assay with a normal rat glomerular RNA and a glomerular RNA of rats with a puromycin aminonucleoside nephropathy, a mimic of a human minimal change type nephrotic syndrome. Then we have found that synaptic vesicle protein 2B, ephrin-B1 and neurexin were already downregulated at the early stage of puromycin aminonucleoside nephropathy, and that these molecules were localized close to nephrin. It is conceivable that these molecules are the slit diaphragm associated molecules, which participate in the regulation of the barrier function. These molecules could be targets to establish a novel therapy for nephrotic syndrome. Core tip: The slit diaphragm located between neighboring foot processes of a glomerular podocyte functions as a final barrier to retain plasma proteins. Recently several molecules such as nephrin and podocin were identified as functional molecules of the slit diaphragm. However, the precise molecular compositions of the slit diaphragm are still unclear and the mechanism regulating its barrier function is not fully understood yet. Recently we have reported that synaptic vesicle protein 2B, ephrin-B1 and neurexin are expressed in podocyte and the decreased function of these molecules participates in the initiation of proteinuria. These molecules could be targets for a novel therapy for proteinuria.Fukusumi Y, Miyauchi N, Hashimoto T, Saito A, Kawachi H. Therapeutic target for nephrotic syndrome: Identification of novel slit diaphragm associated molecules.

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Synaptic Vesicle Protein SV2B, but Not SV2A, Is Predominantly Expressed and Associated with Microvesicles in Rat Pinealocytes

Cited by 32 publications

References 33 publications

SV2A and SV2C are not vesicular Ca2+ transporters but control glucose-evoked granule recruitment

SV2A and SV2C are not vesicular Ca2+ transporters but control glucose-evoked granule recruitment

Synaptic Vesicle Protein 2B Is Expressed in Podocyte, and Its Expression Is Altered in Proteinuric Glomeruli

Therapeutic target for nephrotic syndrome: Identification of novel slit diaphragm associated molecules

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