Synaptic vesicles isolated from the electric organ of Torpedo californica and from the central nervous system of Mus musculus contain small ribonucleic acids (sRNAs)

Abstract

Synaptic vesicles (SVs) are presynaptic organelles that load and release small molecule neurotransmitters at chemical synapses. In addition to classic neurotransmitters, we have demonstrated that SVs isolated from the Peripheral Nervous Systems (PNS) of the electric organ of Torpedo californica, a model cholinergic synapse, and SVs isolated from the Central Nervous System (CNS) of Mus musculus (mouse) contain small ribonucleic acids (sRNAs; ≤ 50 nucleotides) (Scientific Reports, 5:1–14(14918) Li et al. (2015) [1]). Our previous publication provided the five most abundant sequences associated with the T. californica SVs, and the ten most abundant sequences associated with the mouse SVs, representing 59% and 39% of the total sRNA reads sequenced, respectively). We provide here a full repository of the SV sRNAs sequenced from T. californica and the mouse deposited in the NCBI as biosamples. Three data studies are included: SVs isolated from the electric organ of T. californica using standard techniques, SVs isolated from the electric organ of T. californica using standard techniques with an additional affinity purification step, and finally, SVs isolated from the CNS of mouse. The three biosamples are available at https://www.ncbi.nlm.nih.gov/biosample/ SRS1523467, SRS1523466, and SRS1523472 respectively.

Costorage of High Molecular Weight Neurotransmitters in Large Dense Core Vesicles of Mammalian Neurons

“…Secretory vesicles are produced along the regulated secretory pathway and store soluble proteins, peptides or low molecular weight (MW) neurotransmitters. Very recent studies have demonstrated that they may also contain small ribonucleic acids (sRNAs), at least in the electric organ of Torpedo californica and in mouse synapses (Kim et al, 2015 ; Li et al, 2015 , 2017 ; Gümürdü et al, 2017 ). Once assembled, secretory vesicles are actively transported to specific subcellular domains for extracellular delivery in response to appropriate signals.…”