Synbiotic effect of <i>Lactobacillus helveticus</i> M92 and prebiotics on the intestinal microflora and immune system of mice

Frece, Jadranka; Kos, Blaženka; Svetec, Ivan‐Krešimir; Zgaga, Zoran; Beganović, Jasna; Leboš, Andreja; Šušković, Jagoda

doi:10.1017/s0022029908003737

Cited by 68 publications

(54 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further important characteristics of probiotics are their own adherence to intestinal epithelial cells, as well as the prevention of the adhesion of pathogenic bacteria (41,42). Adherence of probiotic bacteria to the intestinal epithelium is an important characteristic as it promotes colonisation and long-time persistence.…”

Section: Discussionmentioning

confidence: 99%

Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium

Potočnjak

Pušić

Frece

et al. 2017

Food Technol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

SummaryThe benefi ts of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new Lactobacillus plantarum strains isolated from diff erent dairy products: cow′s cheese, sheep′s cheese and whey. In order to determine the antibacterial activity of yet unexplored L. plantarum strains against Salmonella enterica serotype Typhimurium, in vitro competition and co-culture tests were done. Furthermore, adhesion of these strains to Caco-2 cells and their infl uence on the adhesion of Salmonella were tested. Results showed the potential probiotic activity of isolated strains. L. plantarum strains survived in the presence of 1 % bile salts, they possessed acidifi cation ability, antibacterial activity and signifi cantly att enuated the growth of S. Typhimurium in brain heart infusion broth. All tested L. plantarum strains were able to adhere to Caco-2 cells and signifi cantly impair the adhesion of S. Typhimurium. All three L. plantarum strains exhibited signifi cant probiotic potential and anti-Salmonella activity; therefore, further testing on in vivo models should follow.

show abstract

Section: Discussionmentioning

confidence: 99%

Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium

Potočnjak

Pušić

Frece

et al. 2017

Food Technol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Considering their benefi ts, LAB have a great potential for application in functional foods (2). In meat preservation and fermentation, LAB are considered technologically fundamental.…”

Section: Introductionmentioning

confidence: 99%

“…In meat preservation and fermentation, LAB are considered technologically fundamental. They decrease pH with lactic acid, produce bacteriocins that prevent the growth of pathogenic and spoilage microorganisms, modify sensory properties (fl avour, colour and texture) of raw material, and improve the safety, stability and shelf-life of meat products (2,3).…”

Section: Introductionmentioning

confidence: 99%

Lactococcus lactis ssp. lactis as Potential Functional Starter Culture

Frece

Cvrtila

Topić

et al. 2014

Food. Technol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

All experiments were carried out on fi ve samples of homemade horsemeat sausage. SummaryThe aim of this study is to identify and characterise potential autochthonous functional starter cultures in homemade horsemeat sausage. The dominant microfl ora in the samples of horsemeat sausage were lactic acid bacteria (LAB), followed by micrococci. Among the LAB, Lactococcus lactis ssp. lactis and Lactobacillus plantarum were the dominant species, and since the fi rst is not common in fermented sausages, we characterised it as a potential functional starter culture. Lactococcus lactis ssp. lactis produced a signifi cant amount of lactic acid, displayed good growth capability at 12, 18 and 22 °C, growth in the presence of 5 % NaCl, good viability a er lyophilisation and in simulated gastric and small intestinal juice, antimicrobial activity against test pathogens, and good adhesive properties in vitro.

show abstract

“…Despite this potential, however, there are few in vivo human or animal studies providing evidence that prebiotics can be used to support specific probiotic strains. Unfortunately, most synbiotic studies, including work in rats (44)(45)(46)(47)(48)(49), mice (50)(51)(52), pigs (53)(54)(55)(56)(57), chickens (58,59), and humans (60), did not employ strainspecific detection methods and therefore did not provide information on the potential synergism between pre-and probiotics. Of the in vivo studies that did discriminate the probiotic strain, most still did not demonstrate that in vivo performance could be enhanced by a prebiotic.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Selection To Identify Bacterial Strains with Enhanced Ecological Performance in Synbiotic Applications

Krumbeck

Maldonado-Gómez

Martínez

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

One strategy for enhancing the establishment of probiotic bacteria in the human intestinal tract is via the parallel administration of a prebiotic, which is referred to as a synbiotic. Here we present a novel method that allows a rational selection of putative probiotic strains to be used in synbiotic applications: in vivo selection (IVS). This method consists of isolating candidate probiotic strains from fecal samples following enrichment with the respective prebiotic. To test the potential of IVS, we isolated bifidobacteria from human subjects who consumed increasing doses of galactooligosaccharides (GOS) for 9 weeks. A retrospective analysis of the fecal microbiota of one subject revealed an 8-fold enrichment in Bifidobacterium adolescentis strain IVS-1 during GOS administration. The functionality of GOS to support the establishment of IVS-1 in the gastrointestinal tract was then evaluated in rats administered the bacterial strain alone, the prebiotic alone, or the synbiotic combination. Strain-specific quantitative real-time PCR showed that the addition of GOS increased B. adolescentis IVS-1 abundance in the distal intestine by nearly 2 logs compared to rats receiving only the probiotic. Illumina 16S rRNA sequencing not only confirmed the increased establishment of IVS-1 in the intestine but also revealed that the strain was able to outcompete the resident Bifidobacterium population when provided with GOS. In conclusion, this study demonstrated that IVS can be used to successfully formulate a synergistic synbiotic that can substantially enhance the establishment and competitiveness of a putative probiotic strain in the gastrointestinal tract.T he mechanistic role of the gastrointestinal (GI) microbiota and its metabolites in maintaining human health has been well demonstrated (1-3). Gut microbes provide several important benefits for their host, including provision of nutrients, development and maturation of the immune system, and protection against pathogens via colonization resistance (4). However, the gut microbiota may also contribute to obesity, inflammatory and autoimmune diseases, and other chronic disease states (5-7). Such diseases are often associated with compositional alterations in the fecal microbiota, a condition referred to as "dysbiosis" (8). Given that the presence of specific types of bacteria and their relative abundance within the gut are considered to affect host health, there is much interest in devising strategies that modulate gut microbiota composition and potentially redress disease-related dysbiotic patterns (9).Dietary approaches currently available to modulate the gut microbiota include prebiotics (10-12), fermentable fibers (13, 14), probiotics (or live biotherapeutics) (15), and synbiotics, which are a combination of a probiotic and a prebiotic (11, 16). According to Kolida and Gibson (16), synbiotics can be either complementary or synergistic. Complementary synbiotics consist of a probiotic and a prebiotic selected to independently confer benefits to the host. In contrast, sy...

show abstract

Synbiotic effect of Lactobacillus helveticus M92 and prebiotics on the intestinal microflora and immune system of mice

Cited by 68 publications

References 21 publications

Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium

Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium

Lactococcus lactis ssp. lactis as Potential Functional Starter Culture

In Vivo Selection To Identify Bacterial Strains with Enhanced Ecological Performance in Synbiotic Applications

Contact Info

Product

Resources

About