2023
DOI: 10.1371/journal.pone.0281330
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Synchronization of the ovulation and copulation timings increased the number of in vivo fertilized oocytes in superovulated female mice

Abstract: The number of sperm that reaches the oocytes in mammalian species is limited. In mice, 8–10 oocytes are ovulated, a similar number of sperm reaches the oocytes, and nearly all oocytes are fertilized via natural mating. Meanwhile, our improved superovulation technique (ultrasuperovulation: administration of inhibin antiserum and equine chorionic gonadotropin [IASe]) produced 100 oocytes from a single female C57BL/6 mouse but resulted in only approximately 20 fertilized oocytes via mating. We hypothesized that s… Show more

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Cited by 5 publications
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