2016
DOI: 10.1021/acs.analchem.6b01490
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Synchronized Polarization Induced Electrospray: Comprehensively Profiling Biomolecules in Single Cells by Combining both Positive-Ion and Negative-Ion Mass Spectra

Abstract: In this work, a synchronized polarization induced electrospray ionization (SPI-ESI) method is developed and applied for the analysis of single-cell samples. In SPI-ESI, periodic alternating current square wave voltage (AC-SWV) is applied to induce the bipolar spray and both positive-ion and negative-ion mass spectra are obtained through one measurement by synchronizing the mode of mass analyzer with the bipolar spray process. Compared with conventional nanoelectrospray ionization (nESI, flow rate< 1000 nL/min)… Show more

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Cited by 26 publications
(23 citation statements)
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“…A high voltage of 0.75 kV was then applied to perform ESI from a bare nanopipette filled with 5 μ m TEMPO in acetonitrile water (1:1). As was shown in the Supporting Information, Figure S4c, no electrooxidized product, TEMPO + , was observed (peak at m / z 157.1465 was ascribed to the protonated TEMPO) even after a spray time of 30 min (Supporting Information, Figure S5), which can be attributed to the ultralow solution consuming of nESI (approximately 30 nL per minute by our previous measurement under very similar conditions) . Therefore, the EC reaction on the feeder electrode has negligible interferences on accurate examination of that on the wireless bipolar electrode.…”
Section: Figuresupporting
confidence: 57%
See 1 more Smart Citation
“…A high voltage of 0.75 kV was then applied to perform ESI from a bare nanopipette filled with 5 μ m TEMPO in acetonitrile water (1:1). As was shown in the Supporting Information, Figure S4c, no electrooxidized product, TEMPO + , was observed (peak at m / z 157.1465 was ascribed to the protonated TEMPO) even after a spray time of 30 min (Supporting Information, Figure S5), which can be attributed to the ultralow solution consuming of nESI (approximately 30 nL per minute by our previous measurement under very similar conditions) . Therefore, the EC reaction on the feeder electrode has negligible interferences on accurate examination of that on the wireless bipolar electrode.…”
Section: Figuresupporting
confidence: 57%
“…As was shown in the Supporting Information, Figure S4c, no electrooxidized product, TEMPO + , was observed (peak at m/z 157.1465 was ascribed to the protonated TEMPO) even after a spray time of 30 min (Supporting Information, Figure S5), which can be attributed to the ultralow solution consuming of nESI (approximately 30 nL per minute by our previous measurement under very similar conditions). [19] Therefore, the EC reaction on the feeder electrode has negligible interferences on accurate examination of that on the wireless bipolar electrode. When a BUME nanopipette was employed, the TEMPO + cation (Supporting Information, Figure S4d and S6, peak at 156.1387, theoretical m/z 156.1383, Dm = 0.4 mDa) was immediately observed.…”
mentioning
confidence: 99%
“…Hu et al developed a synchronized polarization induced electrospray ionization method for dual polarity MS profiling of PC-12 cells and Alium cepa cells. 130 A periodic, alternating current square wave voltage was applied to induce bipolar spray, synchronized with the mass analyzer. This resulted in positive-ion and negative-ion mass spectra for several metabolites and lipids.…”
Section: Reviewmentioning
confidence: 99%
“…These analytical tools enable the unbiased and label-free identification of diverse types of metabolites in single cells (reviewed in references 1620 ). For example, matrix-assisted laser desorption ionization (MALDI) 21 , secondary ion MS (SIMS) 22 , and nanostructure initiator MS (NIMS) 23 were used to detect small molecules in single plant or animal cells in a vacuum, whereas live single cells were measured under ambient conditions using direct analysis approaches, such as live single-cell video MS 24, 25 , single-probe MS 26 , laser ablation electrospray ionization (LAESI) MS 27 , and variants of microsampling with direct electrospray ionization (ESI) 2830 . To aid molecular identifications, a subset of single-cell analysis approaches added a separation step prior to metabolite detection.…”
Section: Introductionmentioning
confidence: 99%