Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?

Pacheco, María Emilia; Bruzzone, Liliana

doi:10.1016/j.jlumin.2012.12.056

Cited by 85 publications

(49 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing synchronous fluorescence spectroscopy and conventional fluorescence spectroscopy, synchronous fluorescence spectrometry shows good selectivity, high sensitivity, simplified spectra, narrowed bands and less interference etc. . Conventional fluorescence spectroscopy is a commonly used method for studying the reaction mechanisms of substances with bovine serum albumin, but research using a synchronous fluorescence method has been less reported.…”

Section: Introductionmentioning

confidence: 99%

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Liu

Zhang

et al. 2015

Luminescence

View full text Add to dashboard Cite

The reaction mechanism of cefoxitin sodium with bovine serum albumin was investigated using fluorescence spectroscopy and synchronous fluorescence spectroscopy at different temperatures. The results showed that the change of binding constant of the synchronous fluorescence method with increasing temperature could be used to estimate the types of quenching mechanisms of drugs with protein and was consistent with one of fluorescence quenching method. In addition, the number of binding sites, type of interaction force, cooperativity between drug and protein and energy-transfer parameters of cefoxitin sodium and bovine serum albumin obtained from two methods using the same equation were consistent. Electrostatic force played a major role in the conjugation reaction between bovine serum albumin and cefoxitin sodium, and the type of quenching was static quenching. The primary binding site for cefoxitin sodium was sub-hydrophobic domain IIA, and the number of binding sites was 1. The value of Hill's coefficients (nH ) was approximately equal to 1, which suggested no cooperativity in the bovine serum albumin-cefoxitin sodium system. The donor-to-acceptor distance r < 7 nm indicated that static fluorescence quenching of bovine serum albumin by cefoxitin sodium was also a non-radiation energy-transfer process. The results indicated that synchronous fluorescence spectrometry could be used to study the reaction mechanism between drug and protein, and was a useful supplement to the conventional method. Copyright © 2015 John Wiley & Sons, Ltd.

show abstract

Section: Introductionmentioning

confidence: 99%

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Liu

Zhang

et al. 2015

Luminescence

View full text Add to dashboard Cite

show abstract

“…[38][39][40] The fluorescence measured in this study was calibrated to correct the "inner filter" effect. [41] As shown in Figures 5b and S6, fluorescence intensity of BSA (350 nm) quenched quickly with the increase of complex, and accompanied by a dose-dependent manner. The probable quenching mechanism (dynamic or static quenching mechanism) was interpreted using the classical Stern-Volmer equation [42] (Equation 1):…”

Section: Bsa Binding Studiesmentioning

confidence: 73%

Triphenylamine and carbazole‐modified iridium^III2‐phenylpyridine complexes: Synthesis, anticaner application and targeted research

Chen

Liu

Tian

et al. 2019

Applied Organom Chemis

View full text Add to dashboard Cite

Four half‐sandwich iridiumIII (IrIII) triphenylamine or carbazole‐modified 2‐phenylpyridine (TPA/Cz‐PhPy) complexes ([(η5‐Cp*)Ir(C^N)Cl]) were synthesized and characterized. Compared with cisplatin, these complexes show higher activity to A549, HepG2 and HeLa cells, with the IC50 values changed from 2.5 ± 0.1 μM to 14.8 ± 2.6 μM. Additionally, complexes could effectively prevent the migration of cancer cells. IrIII TPA/Cz‐PhPy complexes could bind to protein and transport through serum protein, catalyze the oxidation of nicotinamide‐adenine dinucleotid (NADH) and induce the accumulation of reactive oxygen species, and eventually lead to apoptosis, which was also confirmed by flow cytometry. Moreover, prominent targeted fluorescence property confirmed that IrIII TPA/Cz‐PhPy complexes were involved in non‐energy dependent intracellular uptake mechanism, effectively accumulated in lysosomes and damage the integrity of acidic lysosomes, and eventually induce cell death. Above all, TPA/Cz‐appended half‐sandwich IrIII phenylpyridine complexes are promising anticancer agents with dual functions, including migration inhibition and lysosomal damage.

show abstract

“…Fluorescence quenching was used to further understand the nature of complexes 1–4 binding to BSA, and the data of which were calibrated and defined to correct the “inner filter” effect . As shown in Figure B and Figure S15, fluorescence intensity of BSA quenched quickly with the increase of complexes, and accompanied by a dose‐dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Half‐sandwich Ruthenium (II) complexes with triphenylamine modified dipyridine skeleton and application in biology/luminescence imaging

Ge¹,

Liu²,

Tian³

et al. 2019

Applied Organom Chemis

View full text Add to dashboard Cite

Four half-sandwich ruthenium II (Ru II ) complexes with triphenylaminemodifed dipyridine frameworks were synthesized and characterized. The cytotoxicity of target complexes toward A549 (lung cancer cells), HeLa (cervical cancer cells) and HepG2 (hepatoma cells) were obtained by the MTT assay, which were superior to cisplatin with the IC 50 values changed from 2.4 ± 0.1 μM to 9.2 ± 2.7 μM. Meanwhile, complexes possess the ability of antimetastasis to cancer cells. Ru II complexes could be transported by serum albumin, catalyze the conversion of NADH (the reduced state of nicotinamide-adenine dinucleotide) to NAD + and induce the accumulation of reactive oxygen species, which confirmed the antineoplastic mechanism of oxidation. Ru II complexes could enter A549 cells followed by a non-energy dependent cellular uptake mechanism, target lysosomes with the Pearson's colocalization coefficient of 0.75, lead to lysosomal damage, disturb the cell cycle (S phase), and eventually induce apoptosis. The results demonstrate that these Ru II complexes are potential anticancer agents with dual functions, including metastasis inhibition and lysosomal damage.

show abstract

Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?

Cited by 85 publications

References 47 publications

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Triphenylamine and carbazole‐modified iridium^III2‐phenylpyridine complexes: Synthesis, anticaner application and targeted research

Half‐sandwich Ruthenium (II) complexes with triphenylamine modified dipyridine skeleton and application in biology/luminescence imaging

Contact Info

Product

Resources

About

Synchronous fluorescence spectrometry: Conformational investigation or inner filter effect?

Cited by 85 publications

References 47 publications

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy

Triphenylamine and carbazole‐modified iridiumIII2‐phenylpyridine complexes: Synthesis, anticaner application and targeted research

Half‐sandwich Ruthenium (II) complexes with triphenylamine modified dipyridine skeleton and application in biology/luminescence imaging

Contact Info

Product

Resources

About

Triphenylamine and carbazole‐modified iridium^III2‐phenylpyridine complexes: Synthesis, anticaner application and targeted research