Synchrotron-based infrared microspectroscopy study on the radiosensitization effects of Gd nanoparticles at megavoltage radiation energies

Abstract

Synchrotron-based infrared microspectroscopy is a powerful technique for disentangling biochemical effects in nanoparticle-based radiotherapy approaches.

A synchrotron-based infrared microspectroscopy study on the cellular response induced by gold nanoparticles combined with X-ray irradiations on F98 and U87-MG glioma cell lines

“…This shift has been observed in our previous work using gadolinium NP. 21,27 We also observe changes in the distribution of the relative intensities of several DNA spectral bands (PhI/AII, PhII/AII), as shown in Fig. 6 (top).…”

“…cell fixation and deposition on infrared transparent calcium fluoride (CaF 2 ) cover glasses. 21,27 The cells were fixed at two post-irradiation times: after the irradiation (T 0h ) to evaluate immediate treatmentinduced effects, and after 24 hours (T 24h ) to assess the cellular response from the treatment.…”

“…[44][45][46] An amplification in the apoptotic response of irradiated cells in the presence of AuNP is in accordance with our previous FTIRM study using gadolinium NP. 21,27 The protein modifications as a function of the treatment are clearly shown on the vector normalized Savitzky-Golay second derivative plot ( Fig. 9, left).…”

Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment

“…The live-cell FTIR measurements were crucial for this study to avoid invasive preparation protocols and prevent denaturation of the essential bio-macromolecules and other chemical changes by the usual drying or fixation protocol. Till now, FTIR measurements of gliomas have been carried out in fixed and dried cells and thin tissue slices with the spatial resolution of ∼10 × 10 μm 2 . ,, The advantages of a live-cell setup at the SISSI Chemical and Life Sciences branch line (SISSI-Bio) at the Elettra light source have been published previously. − The setup guarantees to perform microspectroscopy of live cells keeping the compatibility with the physiological sample environment; its exploitation is essential for fulfilling the goals of this study.…”

“…Recent studies showed that treatment with riluzole suppresses the proliferation of many cancer cells, as it possesses antineoplastic properties against melanoma, glioma, breast, and prostate cancer by decreasing the release of glutamate (reviewed in ref ). Besides, there is also evidence that riluzole can upturn the cancer response to chemotherapy and radiotherapy. − The clinical outcome of radiotherapy, alone or combined with nanoparticles as radiosensitizers, was previously analyzed in F98 and U87MG cell lines using Fourier transform infrared (FTIR) microspectroscopy by our group. − We proved the enhanced effectiveness of tumor-cell-killing when tumor cells loaded with nanoparticles were exposed to irradiation. − Synchrotron radiation (SR)-FTIR microspectroscopy was used for the characterization of glioma blood vessels to define biomolecular markers of these tumors, and it was verified that the synchrotron light provided advantages over a globar IR source for detailed spectral analysis of such subtle features . Chemometric analyses, namely, principal component analysis (PCA) and hierarchical cluster analysis (HCA), showed that the brightness of a SR-IR source provides a more satisfying signal quality compared with the conventional source to retrieve the subtle molecular markers of glioma, characterizing them from the surrounding tissue .…”