Synchrotron CT of an equine digit at the Australian Synchrotron Imaging and Medical Beamline

Abstract

Laminitis is an extremely painful and debilitating condition of horses that can affect their athletic ability and even quality of life. The current gold standard for assessment of laminar tissue is histology, which is the only modality that enables detailed visualization of the lamina. Histology requires dissection of the hoof and therefore can only represent one specific time point. The superior spatial and contrast resolution of synchrotron computed tomography (sCT), when compared with readily available imaging modalities, such as radiographs and conventional CT, provides an opportunity for detailed studies of the lamina without the need for hoof dissection and histological assessment. If the resolution of histology can be matched or even approached, dynamic events, such as laminar blood flow, could also be studied on the microscopic tissue level. To investigate this possible application of sCT further, two objectives are presented: (i) to develop a protocol for sCT of an equine digit using cadaver limbs and (ii) to apply the imaging protocol established during (i) for sCT imaging of the vasculature within the foot using an ex vivo perfusion system to deliver the vascular contrast. The hypotheses were that sCT would allow sufficient resolution for detailed visualization to the level of the secondary lamellae and associated capillaries within the equine digit. Synchrotron CT enabled good visualization of the primary lamellae (average length 3.6 mm) and the ex vivo perfusion system was able to deliver vascular contrast agent to the vessels of the lamina. The individual secondary lamellae (average length 0.142 mm) could not be seen in detail, although differentiation between primary and secondary lamellae was achieved. This approaches, but does not yet reach, the current gold standard, histology, for assessment of the lamellae; however, with further refinement of this imaging technique, improved resolution may be accomplished in future studies.

The SmARTR pipeline: a modular workflow for the cinematic rendering of 3D scientific imaging data

“…In this study, we used CT for the pipeline example, and our samples were stained with either 1% I2E, 0.7% PTA, or a combination of both compounds for dissected organs. However, alternative staining agents or methods beyond submersion 30,[37][38][39][145][146][147][148] , known to maximize soft tissue contrast in particular samples or to enhance specific organ differentiation, may be employed (Figure 1, step 2). Regarding specimen mounting, we devised a simple methodology (Figure 1, step 3) to position samples, enabling optimal imaging and rendering conditions while preserving sample integrity in terms of both morphology and anatomy.…”