Syncytial hepatitis of farmed tilapia, <i><scp>O</scp>reochromis niloticus</i> (<scp>L</scp>.): a case report

Ferguson, H. W.; Kabuusu, Richard M.; Beltrán, Sandra; Reyes, Eugenio; Lince, J A; Pozo, Jorge del

doi:10.1111/jfd.12142

Cited by 166 publications

(256 citation statements)

References 15 publications

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“…To further examine the developed nested RT-PCR, we applied it to detect TiLV RNA in liver samples preserved in RNAlater reagent, which were taken from South American tilapia showing signs of syncytial hepatitis (11,12), or from healthy controls. This test was performed in a blinded way using the following procedure: the presence or absence of TiLV RNA in the samples was tested by RT-PCR (12) at St. George's University, Grenada.…”

Section: Resultsmentioning

confidence: 99%

“…High mortalities were also observed in naive tilapia exposed to an isolate of TiLV (10). Tilapia mortality, suspected of having a viral etiology, has also been described in Ecuador (11,12). Although variations in pathological presentation have been described (where lesions were focused in the central nervous system or in the liver, in Israel or Ecuador, respectively), sequencing the whole genome of TiLV revealed that tilapia in the two countries were infected with almost identical viruses (4).…”

mentioning

confidence: 99%

“…TiLV outbreaks are characterized by high mortalities and economical losses (10,11), and no vaccines against TiLV are currently available. Thus, there is a great need for the implementation of prompt control measures: culling of infected stocks, setting quarantine, restricting trades, and control of possible vectors.…”

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confidence: 99%

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Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR

Tsofack

Zamostiano

Watted³

et al. 2017

J Clin Microbiol

131

115

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Tilapia are an important group of farmed fish that serve as a significant protein source worldwide. In recent years, substantial mortality of wild tilapia has been observed in the Sea of Galilee and in commercial ponds in Israel and Ecuador. We have identified the etiological agent of these mass die-offs as a novel orthomyxo-like virus and named it tilapia lake virus (TiLV). Here, we provide the conditions for efficient isolation, culturing, and quantification of the virus, including the use of susceptible fish cell lines. Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the rapid detection of TiLV in fish organs. This assay revealed, for the first time to our knowledge, the presence of TiLV in diseased Colombian tilapia, indicating a wider distribution of this emerging pathogen and stressing the risk that TiLV poses for the global tilapia industry. Overall, the described procedures should provide the tilapia aquaculture industry with important tools for the detection and containment of this pathogen.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR

Tsofack

Zamostiano

Watted³

et al. 2017

J Clin Microbiol

131

115

View full text Add to dashboard Cite

show abstract

“…Recently, a novel RNA virus, called tilapia lake virus (TiLV), has been discovered as the causative virus of high cumulative mortalities (80-90%) in farmed tilapia in Israel, Ecuador, and Colombia Del-Pozo et al, 2017;Eyngor et al, 2014;Ferguson et al, 2014;Tsofack et al, 2016) and 5-15% morality in Egypt (Fathi et al, 2017). Initially, Ferguson et al (2014) described a novel disease with suspected viral etiology, namely syncytial hepatitis of tilapia (SHT) in fingerings of Nile tilapia (Oreochromis niloticus) in Ecuador.…”

Section: Introductionmentioning

confidence: 99%

“…Initially, Ferguson et al (2014) described a novel disease with suspected viral etiology, namely syncytial hepatitis of tilapia (SHT) in fingerings of Nile tilapia (Oreochromis niloticus) in Ecuador. In Israel, massive mortalities of both wild and farmed hybrid tilapia (O. niloticus x O. aureus) have been recorded from all over the country since 2009 and the etiological agent was subsequently identified as TiLV (Eyngor et al, 2014).…”

Section: Introductionmentioning

confidence: 99%