Synergetic osteogenesis of extracellular vesicles and loading RGD colonized on 3D-printed titanium implants

Ma, Shiqing; Li, Xuewen; Hu, Han; Ma, Xinlong; Zhao, Zhezhe; Deng, Shu; Wang, Jie; Zhang, Leyu; Wu, Chenxuan; Liu, Zihao; Wang, Yonglan

doi:10.1039/d2bm00725h

Cited by 14 publications

(7 citation statements)

References 42 publications

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“…Therefore, we created new experimental groups, including the Blank, PLGA, CS-PLGA, CS-PLGA@P, and CS-PLGA@FP groups. The secretion of ALP protein and the formation of calcium nodules are markers of osteogenic differentiation . ALP staining can show the degree of differentiation of BMSCs into osteoblasts preliminarily.…”

Section: Resultsmentioning

confidence: 99%

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Functionalized PLGA Microsphere Loaded with Fusion Peptide for Therapy of Bone Defects

Xu,

Tian,

Zhi

et al. 2024

ACS Biomater. Sci. Eng.

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The challenges in the treatment of extensive bone defects are infection control and bone regeneration. Bone tissue engineering is currently one of the most promising strategies. In this study, a short biopeptide with specific osteogenic ability is designed by fusion peptide technology and encapsulated with chitosan-modified poly(lactic acid–glycolic acid) (PLGA) microspheres. The fusion peptide (FP) mainly consists of an osteogenic functional sequence (P-15) and a bone-specific binding sequence (Asp-6), which can regulate bone formation accurately and efficiently. Chitosan-modified PLGA with antimicrobial and pro-healing effects is used to achieve the sustained release of fusion peptides. In the early stage, the antimicrobial and soft tissue healing effects can stop the wound infection as soon as possible, which is relevant for the subsequent bone regeneration process. Our data show that CS-PLGA@FP microspheres have antibacterial and pro-cell migration effects in vitro and excellent pro-wound-healing effects in vivo. In addition, CS-PLGA@FP microspheres promote the expression of osteogenic-related factors and show excellent bone regeneration in a rat defect model. Therefore, CS-PLGA@FP microspheres are an efficient biomaterial that can accelerate the recovery of bone defects.

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Section: Resultsmentioning

confidence: 99%

“…The secretion of ALP protein and the formation of calcium nodules are markers of osteogenic differentiation. 45 ALP staining can show the degree of differentiation of BMSCs into osteoblasts preliminarily. Alizarin red staining was used to detect calcified nodules.…”

Section: Evaluation Of Healing-promoting Ability Of Microsphere In Vivomentioning

confidence: 99%

Functionalized PLGA Microsphere Loaded with Fusion Peptide for Therapy of Bone Defects

Xu,

Tian,

Zhi

et al. 2024

ACS Biomater. Sci. Eng.

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“…The titanium‐based implant should be accompanied by a microenvironment that supports osteogenic differentiation and anti‐inflammatory responses, thereby facilitating bone regeneration and osseointegration 21,22 . Studies have shown that bioactive substances such as RGD, OGP, and ECM play a crucial role in bone regeneration by stimulating osteoblast proliferation, differentiation, alkaline phosphatase activity, collagen secretion, and matrix mineralization 23–25 …”

Section: Discussionmentioning

confidence: 99%

“…21,22 Studies have shown that bioactive substances such as RGD, OGP, and ECM play a crucial role in bone regeneration by stimulating osteoblast proliferation, differentiation, alkaline phosphatase activity, collagen secretion, and matrix mineralization. [23][24][25] In this study, a layer-by-layer self-assembly technique was employed to modify the surface of titanium-based materials with MC3T3-E1 protein. This method allowed for the modulation of the material's surface chemistry and microscopic morphology, which in turn influenced the response of osteoblasts upon contact with the material.…”

Section: Analysis Of Osteogenic Capacitymentioning

confidence: 99%

Enhancing osseointegration of titanium implants through MC3T3‐E1 protein‐gelatin polyelectrolyte multilayers

He,

Guo,

Wang

et al. 2024

J Biomed Mater Res

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Titanium and its alloys have found extensive use in the biomedical field, however, implant loosening due to weak osseointegration remains a concern. Improved surface morphology and chemical composition can enhance the osseointegration of the implant. Bioactive molecules have been utilized to modify the surface of the titanium‐based material to achieve rapid and efficient osseointegration between the implant and bone tissues. In this study, the bioactive substance MC3T3‐E1 protein‐gelatin polyelectrolyte multilayers were constructed on the surface of the titanium implants by means of layer‐by‐layer self‐assembly to enhance the strength of the bond between the bone tissue and the implant. The findings of the study indicate that the layer‐by‐layer self‐assembly technique can enhance surface roughness and hydrophilicity to a considerable extent. Compared to pure titanium, the hydrophilicity of TiOH LBL was significantly increased with a water contact angle of 75.0 2.4°. The modified titanium implant exhibits superior biocompatibility and wound healing ability upon co‐culture with cells. MC3T3‐E1 cells were co‐cultured with TiOH LBL for 1, 3, and 5 days and their viability was higher than 85%. In addition, the wound healing results demonstrate that TiOH LBL exhibited the highest migratory ability (243 ± 10 μm). Furthermore, after 7 days of osteogenic induction, the modified titanium implant significantly promotes osteoblast differentiation.

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“…The functionalized fibronectin can strongly bind to cell membrane integrin and activate epithelial barrier structure related signaling pathway to enhance the formation of epithelial barrier structure, which provides a good strategy for improving interface-epithelial integration also be concerned. Optimized plasma polymerization coatings are biocompatible and remain stable when facing surrounding cells, while adsorption of cytokines and extracellular vesicles produced by surrounding cells can endow the coatings with immunomodulatory and tissue regenerative compacity [61][62][63][64]. In terms of this, more studies are need more further studies to better utilize this technique for enhancing the establishment of epithelial barrier.…”

Section: Discussionmentioning

confidence: 99%

Plasma polymerized bio-interface directs fibronectin adsorption and functionalization to enhance “epithelial barrier structure” formation via FN-ITG β1-FAK-mTOR signaling cascade

et al. 2022

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Background Transepithelial medical devices are increasing utilized in clinical practices. However, the damage of continuous natural epithelial barrier has become a major risk factor for the failure of epithelium-penetrating implants. How to increase the “epithelial barrier structures” (focal adhesions, hemidesmosomes, etc.) becomes one key research aim in overcoming this difficulty. Directly targeting the in situ “epithelial barrier structures” related proteins (such as fibronectin) absorption and functionalization can be a promising way to enhance interface-epithelial integration. Methods Herein, we fabricated three plasma polymerized bio-interfaces possessing controllable surface chemistry. Their capacity to adsorb and functionalize fibronectin (FN) from serum protein was compared by Liquid Chromatography-Tandem Mass Spectrometry. The underlying mechanisms were revealed by molecular dynamics simulation. The response of gingival epithelial cells regarding the formation of epithelial barrier structures was tested. Results Plasma polymerized surfaces successfully directed distinguished protein adsorption profiles from serum protein pool, in which plasma polymerized allylamine (ppAA) surface favored adsorbing adhesion related proteins and could promote FN absorption and functionalization via electrostatic interactions and hydrogen bonds, thus subsequently activating the ITG β1-FAK-mTOR signaling and promoting gingival epithelial cells adhesion. Conclusion This study offers an effective perspective to overcome the current dilemma of the inferior interface-epithelial integration by in situ protein absorption and functionalization, which may advance the development of functional transepithelial biointerfaces. Graphical Abstract Tuning the surface chemistry by plasma polymerization can control the adsorption of fibronectin and functionalize it by exposing functional protein domains. The functionalized fibronectin can bind to human gingival epithelial cell membrane integrins to activate epithelial barrier structure related signaling pathway, which eventually enhances the formation of epithelial barrier structure.

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Synergetic osteogenesis of extracellular vesicles and loading RGD colonized on 3D-printed titanium implants

Abstract: Titanium (Ti) and its alloys has been universally used as surgical implants, the clinical need for modifying titanium surfaces to accelerate the early stage osseointegration and prevent implant loosening is...

Cited by 14 publications

References 42 publications

Functionalized PLGA Microsphere Loaded with Fusion Peptide for Therapy of Bone Defects

Functionalized PLGA Microsphere Loaded with Fusion Peptide for Therapy of Bone Defects

Enhancing osseointegration of titanium implants through MC3T3‐E1 protein‐gelatin polyelectrolyte multilayers

Plasma polymerized bio-interface directs fibronectin adsorption and functionalization to enhance “epithelial barrier structure” formation via FN-ITG β1-FAK-mTOR signaling cascade

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