Synergic effect of retinoic acid and extremely low frequency magnetic field exposure on human neuroblastoma cell line BE(2)C

Marcantonio, Pamela; Re, Brunella Del; Franceschini, Alessia; Capri, Miriam; Lukas, Stella; Bersani, Ferdinando; Giorgi, Gianfranco

doi:10.1002/bem.20581

Cited by 31 publications

(24 citation statements)

References 38 publications

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“…ELF-MF exposure of these cells might have triggered spontaneous differentiation responses that were tolerated in the culture and, ultimately, gave rise to detectable epigenetic change. Notably, an enhancement of differentiation upon ELF-MF exposure was reported in neuronal cells242744, where it correlated with a gain of H3K9 acetylation at specific neuronal genes, promoting their activation45.…”

Section: Discussionmentioning

confidence: 92%

ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesis

Manser

Sater

Schmid

et al. 2017

Sci Rep

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Extremely-low-frequency magnetic fields (ELF-MF) have been classified as “possibly carcinogenic” to humans on the grounds of an epidemiological association of ELF-MF exposure with an increased risk of childhood leukaemia. Yet, underlying mechanisms have remained obscure. Genome instability seems an unlikely reason as the energy transmitted by ELF-MF is too low to damage DNA and induce cancer-promoting mutations. ELF-MF, however, may perturb the epigenetic code of genomes, which is well-known to be sensitive to environmental conditions and generally deranged in cancers, including leukaemia. We examined the potential of ELF-MF to influence key epigenetic modifications in leukaemic Jurkat cells and in human CD34+ haematopoietic stem cells undergoing in vitro differentiation into the neutrophilic lineage. During granulopoiesis, sensitive genome-wide profiling of multiple replicate experiments did not reveal any statistically significant, ELF-MF-dependent alterations in the patterns of active (H3K4me2) and repressive (H3K27me3) histone marks nor in DNA methylation. However, ELF-MF exposure showed consistent effects on the reproducibility of these histone and DNA modification profiles (replicate variability), which appear to be of a stochastic nature but show preferences for the genomic context. The data indicate that ELF-MF exposure stabilizes active chromatin, particularly during the transition from a repressive to an active state during cell differentiation.

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Section: Discussionmentioning

confidence: 92%

ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesis

Manser

Sater

Schmid

et al. 2017

Sci Rep

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“…Marcantonio et al . reported that a 50 Hz MF at 1.0 mT did not elicit alterations in cell cycle progression or cellular viability in neuroblastoma BE(2)C cells [41]. Previous studies have investigated the effects of ELF-MF exposure on cellular proliferation in various neurogenic cell types, such as SH-SY5Y cells, but have shown inconsistent results with respect to cellular behaviors [42, 43].…”

Section: Discussionmentioning

confidence: 99%

The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells

Yimaer

Wei

et al. 2017

Journal of Radiation Research

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Epidemiological studies have indicated a possible association between extremely low–frequency magnetic field (ELF-MF) exposure and the risk of nervous system diseases. However, laboratory studies have not provided consistent results for clarifying this association, despite many years of studies. In this study, we have systematically investigated the effects of 50 Hz MF exposure on DNA damage and cellular functions in both neurogenic tumor cell lines (U251, A172, SH-SY5Y) and primary cultured neurogenic cells from rats (astrocytes, microglia, cortical neurons). The results showed that exposure to a 50 Hz MF at 2.0 mT for up to 24 h did not influence γH2AX foci formation (an early marker of DNA double-strand breaks) in any of six different neurogenic cells. Exposure to a 50 Hz MF did not affect cell cycle progression, cell proliferation or cell viability in neurogenic tumor U251, A172 or SH-SY5Y cells. Furthermore, the MF exposure for 24 h did not significantly affect the secretion of cytokines (TNF-α, IL-6 or IL-1β) in astrocytes or microglia, or the phagocytic activity of microglia. In addition, MF exposure for 1 h per day did not significantly influence expression levels of microtubule-associated protein tau, microtubule-associated protein 2, postsynaptic density 95 or gephyrin in cortical neurons, indicating an absence of effects of MF exposure on the development of cortical neurons. In conclusion, our data suggest that exposure to a 50 Hz MF at 2.0 mT did not elicit DNA damage effects or abnormal cellular functions in the neurogenic cells studied.

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“…http://www.ispacs.com/journals/ijcmb/2014/ijcmb-00015/ International Scientific Publications and Consulting Services In addition Wang in 2009 reported that gradient static magnetic fields (GSMF) might inhibit or prevent new blood vessels formation and could be helpful for the treatment of some diseases relevant to pathological angiogenesis [21]. It was also shown that 24 to 48 hours exposure to 50Hz and 1Mt bio electromagnetic field could reduce proliferation of nouroblastoma cells [22] which was able to induce apoptosis, decrease hsp-60, bcl-2 and increase in bax protein level [23]- [25]. Although according to data analysis of this in vitro research inhibitory effects of bio electromagnetic field on angiogenesis bio markers was observed.…”

Section: Discussionmentioning

confidence: 99%

Role of Low Frequency Bio Electromagnetic Field on Angiogenesis Biomarkers in Human Breast Tumor Cell Line

Baharara¹,

Mousavi²

2014

International Journal of Cellular and Molecular Biotechnology

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Many biological processes are affected by electromagnetic fields. Effect of electromagnetic field on proliferation, cell division, wound healing and gene expression were examined. Angiogenesis, new blood vessel formation, which is required for embryonic development and many physiological events, plays a crucial role in the pathobiology of tumors and researches show angiogenesis inhibition could be an effective therapeutic strategy for patients with cancer. In this research, effects of low-frequency electromagnetic field on VEGF-A, VEGFR-2 and MMP gene expression as potent biomarkers of angiogenesis was investigated in human breast cancer cells. MCF-7 cells were grown in RPMI 1640 medium supplemented with 10% FBS and incubated at 37˚C with 5% CO2. After 48h all flasks exposed with electromagnetic field (50Hz, 0.004 T) then total RNA was extracted and cDNA was synthesized using specific primer. Finally synthesized products were analyzed by Real Time PCR to determine expression level of VEGF-A, VEGFR-2 and MMP. Data was analyzed by SPSS software ANOVA and Tukey. Data analysis shows inhibitory effect of low-frequency bio electromagnetic field with 50Hz, 400 gauss intensity in MCF-7 cell line compared with control group. Although all genes did not show same reduction but gene expression in all cases affected by low-frequency electromagnetic field. Significant inhibitory effect on gene expression was for VEGFR-2 with 25% decrease P<0.001. Matrix Metalloproteinase gene level decreased 8% (P˂0.05) while it was noticeable for VEGF-A at P˃0.05. Results indicate decreases in the expression of biomarkers of angiogenesis in the treated samples with lowfrequency electromagnetic field with 400 gauss intensity in MCF-7 cell line compared with control group which may be promising prevention for angiogenesis related diseases.

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Synergic effect of retinoic acid and extremely low frequency magnetic field exposure on human neuroblastoma cell line BE(2)C

Cited by 31 publications

References 38 publications

ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesis

ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesis

The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells

Role of Low Frequency Bio Electromagnetic Field on Angiogenesis Biomarkers in Human Breast Tumor Cell Line

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