Synergic effects of cancer stem cells markers, CD44 and embryonic stem cell transcription factor Nanog, on bladder cancer prognosis

Siddiqui, Zainab; Srivastava, Anand; Sankhwar, Satya Narayan; Dalela, Divakar; Singh, Vineeta; Zaidi, Noorin; Fatima, Nosheen; Bano, I; Anjum, Sadia

doi:10.1080/09674845.2019.1692761

Cited by 13 publications

(10 citation statements)

References 25 publications

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“…On comparing the tumor grades 44 found 100% Nanog expression in grade 3, 14 Also reported that Nanog was expressed in both low-grade and high-grade BCs and higher grade tumors showed intense expression as compared with low grade. Near results were obtained by Siddiqui et al 45 who found that higher tumor stages exhibited 60.6% of Nanog expression while 55.6% were present in lower tumor stages.…”

Section: Discussionmentioning

confidence: 81%

Prognostic Implications of CD24, SOX2, and Nanog Expression in Invasive Urothelial Carcinoma

Abdelbary¹,

Atwa²,

Elfarargy³

et al. 2023

Applied Immunohistochemistry &Amp; Molecular Morphology

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Background: Urinary bladder cancer (BC) is the seventh most common cancer worldwide with the highest incidence rates in Western Europe, North America, and Australia. The most common type of BC is urothelial carcinoma (UC), which represents a significant cause of morbidity and mortality. Objective: The aim of the study was to evaluate the prognostic value of CD24, SOX2, and Nanog in UC cases and the correlation with recurrence and survival. Materials and Methods: In this study, the authors investigated the expression of CD24, SOX2, and Nanog in 80 patients with urinary BC. The clinical significance of the markers was evaluated by assessing the correlation with the clinicopathologic parameters and prognosis. Results: The CD24 expression was positive in 62.5% of the BC patients, there was a significant association between CD24 expression and high grade and stage and lymphovascular invasion (LVI), P (0.002, 0.0010, and 0.001). SOX2 was expressed in 60 patients (75%), the expression was significantly associated with age, stage, grade, LVI, lymph node, and smoking, P (0.016, 0.01, <0.001, 0.003, 0.036, and 0.002), respectively. Nanog expression was positive in 60% of the BC patients. There was a significant association between Nanog expression and age, high grade, high stage, and LVI (P=0.016, <0.001, and 0.003), respectively. Conclusions: A significant relation between CD24, SOX2, and Nanog with the invasive potential of UC. This increase in expression of the 3 markers with the grades and stages of UC suggests that they can play a role in the development of UC, so they can be used in targeted therapy in the future.

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Section: Discussionmentioning

confidence: 81%

Prognostic Implications of CD24, SOX2, and Nanog Expression in Invasive Urothelial Carcinoma

Abdelbary¹,

Atwa²,

Elfarargy³

et al. 2023

Applied Immunohistochemistry &Amp; Molecular Morphology

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“…Among them, NANOG is an important transcription factor involved in the regulation of cell stemness (28). NANOG plays an important role in self-renewal, undifferentiated state and differentiation ability, high tumorigenicity, and resistance to current standard chemotherapy and radiotherapy (29)(30)(31). It can be activated by different transcription factors, such as OCT4 and SOX2 (32,33).…”

Section: Discussionmentioning

confidence: 99%

EMT and Cancer Cell Stemness Associated With Chemotherapeutic Resistance in Esophageal Cancer

Liu

et al. 2021

Front. Oncol.

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Drug resistance often occurs after chemotherapy in esophageal cancer patients, leading to cancer metastasis and recurrence. However, the relationship among cancer cell migration, recurrence and drug resistance in esophageal cancer drug-resistant cells has not been clearly explained. In this study, we constructed paclitaxel (PTX)-resistant esophageal cancer cells to explore the causes of drug resistance and poor prognosis after chemotherapy in esophageal cancer. Colony formation assay was used to evaluate the difference of colony formation between parental cells and drug resistance cells. Microsphere formation assay was used to examine the phenotype of stem cells. Wound healing and Transwell assays were used to detect the migration ability of drug-resistant cells. Western blotting and immunofluorescence assays were used to explore the mechanisms. Finally, we used nude mouse xenograft model to explore the tumor characteristics and the expression of relative proteins to verify our findings in vivo. Our study demonstrated that the cancer cell stemness characteristics exist in drug-resistant esophageal cancer cells, that expressed the biomarkers of stem cells and were prone to epithelial-mesenchymal transition (EMT). Our results suggested that the expression of EMT biomarkers and stemness-related proteins increased in esophageal cancer cells after continuously using chemotherapeutic drugs for a period of time. This study indicated that simultaneously targeting EMT and stemness could be a better strategy for the treatment of esophageal cancer drug resistance.

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“…It also interacts with Nanog, an embryonic stem-cell transcription factor, which inhibits MDR-1 and causes efflux of chemotherapy drugs in a number of tumour types. High CD44 and nannog expression were both associated with decreased survival [81] as independent prognostic factors along with lymph node status. The authors developed a combined CD44/Nanog score and suggest a use in risk stratification and for prognosis.…”

Section: Biomarkersmentioning

confidence: 96%

British Journal of Biomedical Science in 2020. What have we learned?

Brown

Orchard

Rhodes

2020

British Journal of Biomedical Science

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Each year the British Journal of Biomedical Science publishes a 'What have we learned' editorial designed to introduce readers within the major disciplines of laboratory medicine to developments outside their immediate area. In addition it is designed to inform a wider readership of the advances in the diagnosis and treatment of disease. To this end, in 2020 the journal published 39 articles covering the disciplines within Biomedical Science in the 4 issues comprising volume 77. These included a review of COVID-19 in this issue, 27 original articles, 6 Biomedical Science 'In Brief' and 4 case histories. 27 of the articles involved molecular techniques, with one of these comparing results with a mass spectrometry based method. The preponderance of molecular genetic studies gives us a good idea of the likely future direction of the disciplines

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Synergic effects of cancer stem cells markers, CD44 and embryonic stem cell transcription factor Nanog, on bladder cancer prognosis

Cited by 13 publications

References 25 publications

Prognostic Implications of CD24, SOX2, and Nanog Expression in Invasive Urothelial Carcinoma

Prognostic Implications of CD24, SOX2, and Nanog Expression in Invasive Urothelial Carcinoma

EMT and Cancer Cell Stemness Associated With Chemotherapeutic Resistance in Esophageal Cancer

British Journal of Biomedical Science in 2020. What have we learned?

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