Synergies in coupled hydrolysis and fermentation of cellulose using a Trichoderma reesei enzyme preparation and a recombinant Saccharomyces cerevisiae strain

Abstract

We describe a procedure by which filter paper is digested with a cellulolytic enzyme preparation, obtained from Trichoderma reesei cultivated under solid state fermentation conditions and then fermented by a recombinant Saccharomyces cerevisiae strain. The yeast strain produces a β-glucosidase encoded by the BGL1 gene from Saccharomycopsis fibuligera that quantitatively and qualitatively complements the limitations that the Trichoderma enzyme complex shows for this particular activity. The supplemental β-glucosidase activity fuels the progression of cellulose hydrolysis and fermentation by decreasing the inhibitory effects caused by the accumulation of cellobiose and glucose. Fermentation of filter paper by this procedure yields ethanol concentrations above 70 g/L.

Ethanol production using vegetable peels medium and the effective role of cellulolytic bacterial (Bacillus subtilis) pre-treatment

“…Significant elevation of ethanol production rate was observed in the co-fermentation process where cellulosic media were inoculated with cellulolytic bacteria previously. Overall, the method proves the efficiency of the co-fermentation 26– 28 . The economic advantage of using vegetable peels media over molasses is the recycling process of abundant garbage.…”

Bioethanol fermentation from kitchen waste using Saccharomyces cerevisiae

“…Most of the kitchen waste was similar feedstock to lignocellulosic raw materials, which is considered to be an excellent substrate 22 . Previous works delineated the pathway of converting plant-based waste biomass to bioethanol, in which enzyme pretreatment was conducted before yeast fermentation [23][24][25][26] . Gnansounou & Dauriat produced 30.9 g bioethanol and 65.2 L biogas using 1 kg of kitchen waste 27 .…”

Revolutionizing Renewable Resources: Cutting-Edge Trends and Future Prospects in the Valorization of Oligosaccharides

“…This was accomplished by integrating the aglA gene, encoding for glucosidase, with glycophosphatidylinositol (GPI) anchor sequences from the SED1 gene, resulting in a hybrid protein anchored to the yeast cell membrane. This modification improves the stability and efficiency of the enzyme, offering a promising approach for industrial IMO production [170]. These developments underscore the integration of microbiology, genetic engineering, and process optimization in the production of valuable oligosaccharides such as panose.…”

“…Further innovation in panose production involves genetic engineering techniques to enhance efficiency. Casa-Villegas et al [170] made significant advancements by genetically modifying Saccharomyces cerevisiae cells to act as catalytic agents for panose synthesis. This was accomplished by integrating the aglA gene, encoding for glucosidase, with glycophosphatidylinositol (GPI) anchor sequences from the SED1 gene, resulting in a hybrid protein anchored to the yeast cell membrane.…”