Synergism and Antagonism of Essential Oil Fractions of Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris Against Penicillium expansum

Tamgue, Ousman; Louis, Bengyella; Nguefack, Julienne; Do, Jean Blaise Lekagne; Dako, Charles Daboy

doi:10.3923/ijpp.2011.51.62

Cited by 12 publications

(4 citation statements)

References 7 publications

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“…Once the membrane becomes permeable, other essential oil components which target internal organelles (like carvacrol) can then act. This synergy permits the inhibition of the growth of microorganisms [ 44 ]. The fruit and leaf essential oils of S. mombin showed different activities against tested microorganisms inthe antimicrobial assays.…”

Section: Discussionmentioning

confidence: 99%

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Essential Oils from the Fruits and Leaves of Spondias mombin Linn.: Chemical Composition, Biological Activity, and Molecular Docking Study

Ampadu

Mensah

Darko

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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This work focused on characterizing the chemical constituents and evaluating the antioxidant and antimicrobial activities of the essential oils obtained from the fruit and leaves of Spondias mombin—a flowering plant of the Anacardiaceae family. Essential oils were extracted through steam distillation and characterized by gas chromatography-mass spectrometry. For the fruit essential oil, 35 compounds were obtained, and 25 compounds were identified in the leaf essential oil. The dominant compounds present in the fruit essential oil were (E)-ethyl cinnamate (14.06%) and benzyl benzoate (12.27%). Methyl salicylate (13.05%) and heptacosane (12.69%) were the abundant compounds in the leaf essential oil. The antioxidant activity of the essential oils was evaluated via phosphomolybdenum, hydrogen peroxide scavenging, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, and thiobarbituric acid reactive substances (TBARS) assays. The total antioxidant capacity of fruit and leaf essential oils was 48.5 ± 0.7 μg/gAAE and 48.5 ± 0.7 μg/g AAE, respectively. The half maximal scavenging concentrations of the essential oils in the hydrogen peroxide; DPPH and TBARS assays ranged from 252.2 μg/mL to 2288 μg/mL. The antimicrobial activity of the essential oils was tested using broth dilution and disc diffusion assays against eight microorganisms. The essential oils exhibited broad-spectrum antimicrobial activity against the microorganisms with minimum inhibitory concentrations of 9.75–50 mg/mL. Also, the zones of inhibition of the oils ranged from 12 mm to 25 mm. The biofilm inhibitory activities of the oils were dose-dependent with BIC50 values of 42.49 ± 0.1 mg/mL and 97.34 ± 0.6 mg/mL for fruit and leaf essential oils, respectively. Molecular docking studies revealed that the antibiofilm action of the fruit and leaf essential oils could be due to inhibition of the quorum sensing protein, LasR. The results suggest a possible application of the oils as antioxidant and antimicrobial agents.

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Section: Discussionmentioning

confidence: 99%

“…Once the membrane becomes permeable, other essential oil components which target internal organelles (like carvacrol) can then act. is synergy permits the inhibition of the growth of microorganisms [44].…”

Section: Discussionmentioning

confidence: 99%

Essential Oils from the Fruits and Leaves of Spondias mombin Linn.: Chemical Composition, Biological Activity, and Molecular Docking Study

Ampadu

Mensah

Darko

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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show abstract

“…The different morphology between L. monocytogenes and E. coli O157:H7 also affect the mechanism of action of the EOs because the absence of the additional outer membrane and porins on the external peptidoglycan layer makes L. monocytogenes less resistant to antimicrobial agents than E. coli O7:H157. Generally, Gram-negative bacteria are less sensitive to antimicrobials because of the lipopolysaccharide outer membrane of this group, which restricts diffusion of hydrophobic compounds [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Synergistic Antimicrobial Activities of Combinations of Vanillin and Essential Oils of Cinnamon Bark, Cinnamon Leaves, and Cloves

Cava-Roda

Taboada-Rodríguez

López−Gómez

et al. 2021

Foods

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Plant bioactive compounds have antimicrobial and antioxidant activities that allow them to be used as a substitute for synthetic chemical additives in both food and food packaging. To improve its sensory and bactericidal effects, its use in the form of effective combinations has emerged as an interesting possibility in the food industry. In this study, the antimicrobial activities of essential oils (EOs) of cinnamon bark, cinnamon leaves, and clove and the pure compounds vanillin, eugenol, and cinnamaldehyde were investigated individually and in combination against Listeria monocytogenes and Escherichia coli O157:H7. The possible interactions of combinations of pure compounds and EOs were performed by the two-dimensional checkerboard assay and isobologram methods. Vanillin exhibited the lowest antimicrobial activity (MIC of 3002 ppm against L. monocytogenes and 2795 ppm against E. coli O157:H7), while clove and cinnamon bark EOs exhibited the highest antimicrobial activity (402–404 against L. monocytogenes and 778–721 against E. coli O157:H7). For L. monocytogenes, pure compound eugenol, the main component of cinnamon leaves and clove, showed lower antimicrobial activity than EOs, which was attributed to the influence of the minor components of the EOs. The same was observed with cinnamaldehyde, the main component of cinnamon bark EO. The combinations of vanillin/clove EO and vanillin/cinnamon bark EO showed the most synergistic antimicrobial effect. The combination of the EOs of cinnamon bark/clove and cinnamon bark/cinnamon leaves showed additive effect against L. monocytogenes but indifferent effect against E. coli O157:H7. For L. monocytogenes, the best inhibitory effects were achieved by cinnamon bark EO (85 ppm)/vanillin (910 ppm) and clove EO (121 ppm)/vanillin (691 ppm) combinations. For E. coli, the inhibitory effects of clove EO (104 ppm)/vanillin (1006 ppm) and cinnamon leaves EO (118 ppm)/vanillin (979 ppm) combinations were noteworthy. Some of the tested combinations increased the antimicrobial effect and would allow the effective doses to be reduced, thereby offering possible new applications for food and active food packaging.

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“…The synergistic and antagonistic effects between allelochemicals were reported in some works. In fact, Tamgue et al (2011) noted the presence of synergism and antagonism in the essential oil fractions of Cymbopogon citratus, Ocimum gratissium and Thymus vulgaris when tested against Penicillium expansum.…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of Datura metel L. organic and aqueous extracts on some pathogenic and antagonistic fungi

Rinez¹,

Daami‐Remadi²,

Ladhari³

et al. 2013

Afr. J. Microbiol. Res.

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The present study was undertaken to assess the in vitro antifungal activity of petroleum ether, chloroform, methanol and aqueous extracts of Datura metel L. leaves and flowers, against two Trichoderma species (Trichoderma harzianum and Trichoderma viride) and three formae speciales of Fusarium oxysporum, that is, Fusarium oxysporum f. sp. melonis, Fusarium oxysporum f. sp. lycopersici and Fusarium oxysporum f. sp. tuberosi. Radial growth of the pathogen on PDA plates was recorded after 3 to 7 days of incubation at 25°C. Results revealed an important antifungal activity of D. metel leaf and flower aqueous extracts at all concentrations tested (1, 2, 3 and 4%) against pathogenic fungus. However, the extent of response to extracts was species specific. In fact, the highest inhibition (69%) of T. viride mycelial growth was observed with flower aqueous extracts. Indeed, F. oxysporum f. sp. melonis was found to be more susceptible than the other fungal species to organic extract; radial growth inhibition varied from 24 to 76% and from 31 to 76% in the presence of leaf and flower organic extracts, respectively. In addition, the radial growth of F. oxysporum f. sp. Lycopersici was reduced by all organic extracts. The fungicidal activity of leaf extracts was more effective against F. oxysporum f. sp. tuberosi as compared to flower extracts. Both Trichoderma species were less sensitive to D. metel organic extracts than Fusarium species. Our findings showed that D. metel extracts can be used as potential source of fungicides to control the phytopathogenic fungi tested.

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Synergism and Antagonism of Essential Oil Fractions of Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris Against Penicillium expansum

Cited by 12 publications

References 7 publications

Essential Oils from the Fruits and Leaves of Spondias mombin Linn.: Chemical Composition, Biological Activity, and Molecular Docking Study

Essential Oils from the Fruits and Leaves of Spondias mombin Linn.: Chemical Composition, Biological Activity, and Molecular Docking Study

Synergistic Antimicrobial Activities of Combinations of Vanillin and Essential Oils of Cinnamon Bark, Cinnamon Leaves, and Cloves

Antifungal activity of Datura metel L. organic and aqueous extracts on some pathogenic and antagonistic fungi

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