Synergism Between Enzymes Involved in the Solubilization of Native Cellulose

Wood, Thomas M.; McCrae, Sheila I.

doi:10.1021/ba-1979-0181.ch010

Cited by 209 publications

(105 citation statements)

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“…The degradation of cellulose has been the subject of numerous studies (Wood and McCrae, 1979;Ryu et al, 1984;Beldman et al, 1988). Generally, the synergistic action between endoglucanases and CBH is measured by determining the increase in reducing end groups upon degradation of Avicel.…”

Section: Discussionmentioning

confidence: 99%

“…Although severa1 mechanisms to explain this synergism have been proposed (Wood and McCrae, 1979;Ryu et al, 1984;Beldman et al, 1988;Walker and Wilson, 1991), there is still debate conceming the exact mechanism of cellulose hydrolysis. The adsorption behavior of the various endoglucanases on crystalline cellulose seems to play an important role and explains, at least qualitatively, the difference in optimal endoglucanase/CBH ratio in solution (Ryu et al, 1984;Beldman et al, 1987Beldman et al, , 1988.…”

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confidence: 99%

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The Effect of Xyloglucans on the Degradation of Cell-Wall-Embedded Cellulose by the Combined Action of Cellobiohydrolase and Endoglucanases from Trichoderma viride

Vincken¹,

Beldman²,

Voragen³

1994

Plant Physiol.

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l w o endoglucanases of Trichoderma viride, endol and endolV, were assayed for their activity toward alkali-extracted apple xyloglucans. EndolV was shown to have a 60-fold higher activity toward xyloglucan than endol, whereas carboxymethyl cellulose and crystalline cellulose were better substrates for the latter. l h e enzymic degradation of cellulose embedded in the complex cell-wall matrix of apple fruit tissue has been studied using cellobiohydrolase (CBH) and these two different endoglucanases. A high-performance liquid chromatographic method (Aminex HPX-22H) was used to monitor the release of cellobiose and oligomeric xyloglucan fragments. Synergistic action between CBH and endoglucanases on cell-wallembedded cellulose was, with respect to their optimal ratio, slightly different from that reported for crystalline cellulose. l h e combination of endolv and CBH solubilized twice as much cellobiose compared to a combination of endol and CBH. Apparently, the concomitant removal of the xyloglucan coating from cellulose microfibrils by endolV is essential for an efficient degradation of cellulose in a complex matrix. Cellulose degradation slightly enhanced the solubilization of xyloglucans. lhese results indicate optimal degradation of cell-wall-embedded cellulose by a threeenzyme system consisting of an endoglucanase with high affinity toward cellulose (endol), a xyloglucanase (endolV), and CBH.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The Effect of Xyloglucans on the Degradation of Cell-Wall-Embedded Cellulose by the Combined Action of Cellobiohydrolase and Endoglucanases from Trichoderma viride

Vincken¹,

Beldman²,

Voragen³

1994

Plant Physiol.

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“…Synergism is said to occur when the activity exhibited by mixtures of components is greater than the sum of the activity of these components evaluated separately (Walker and Wilson, 1991;Wood and McCrae, 1979;Wood and Garcia-Campayo, 1990;Woodward, 1991). Quantitative representation of the extent of synergism is usually expressed in terms of a ''degree of synergism'' (DS)-equal to the ratio of the activity exhibited by mixtures of components divided by the sum of the activities of separate components.…”

Section: Synergismmentioning

confidence: 99%

Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems

Zhang

Lynd

2004

Biotech & Bioengineering

1,658

1,284

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Information pertaining to enzymatic hydrolysis of cellulose by noncomplexed cellulase enzyme systems is reviewed with a particular emphasis on development of aggregated understanding incorporating substrate features in addition to concentration and multiple cellulase components. Topics considered include properties of cellulose, adsorption, cellulose hydrolysis, and quantitative models. A classification scheme is proposed for quantitative models for enzymatic hydrolysis of cellulose based on the number of solubilizing activities and substrate state variables included. We suggest that it is timely to revisit and reinvigorate functional modeling of cellulose hydrolysis, and that this would be highly beneficial if not necessary in order to bring to bear the large volume of information available on cellulase components on the primary applications that motivate interest in the subject. B

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“…Filamentous fungi like Aspergillus niger and Hypocrea jecorina (Trichoderma reesei) have been shown to produce a wide spectrum of polysaccharide-hydrolytic enzymes. Of these fungi, A. niger is industrially used as a producer of many pectinases (Bussink et al 1992;Delgado et al 1992;Parenicová et al 2000) and hemicellulosedegrading enzymes, like xylanases and arabinases (Gielkens et al 1997;van Peij et al 1997), while H. jecorina is the most important organism used in cellulase production (Fägerstam and Pettersson 1980;Irwin et al 1993;Nidetzky et al 1994;Wood and McCrae 1979).…”

Section: Introductionmentioning

confidence: 99%

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)

Stricker

Mach

Graaff

2008

Appl Microbiol Biotechnol

249

182

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Synergism Between Enzymes Involved in the Solubilization of Native Cellulose

Cited by 209 publications

References 0 publications

The Effect of Xyloglucans on the Degradation of Cell-Wall-Embedded Cellulose by the Combined Action of Cellobiohydrolase and Endoglucanases from Trichoderma viride

The Effect of Xyloglucans on the Degradation of Cell-Wall-Embedded Cellulose by the Combined Action of Cellobiohydrolase and Endoglucanases from Trichoderma viride

Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)

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