Synergism of biochemical and mechanical stimuli in the differentiation of human placenta-derived multipotent cells into endothelial cells

Wu, Chia Ching; You, Chao; Chen, Cheng Nan; Chien, Shu; Chen, Yao Chang; Chien, Chih Chung; Chiu, Jeng Jiann; Yen, B. Linju

doi:10.1016/j.jbiomech.2007.11.008

Cited by 83 publications

(74 citation statements)

References 24 publications

(28 reference statements)

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“…We also found vWF expression in the MSCs after exposure to shear stress (data not shown). This result was consistent with the findings from other studies that examined the effect of shear stress on cell differentiation [13,14,23,24,28] . The present study suggests that shear stress induces differentiation along an endothelial lineage in canine MSCs.…”

Section: Discussionsupporting

confidence: 93%

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

Dong

et al. 2009

Acta Pharmacol Sin

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Aim: Recent studies have demonstrated that mesenchymal stem cells (MSCs) can differentiate into endothelial cells. The effect of shear stress on MSC differentiation is incompletely understood, and most studies have been based on two-dimensional systems. We used a model of tissue-engineered vascular grafts (TEVGs) to investigate the effects of shear stress on MSC differentiation. Methods: MSCs were isolated from canine bone marrow. The TEVG was constructed by seeding MSCs onto poly-ε-caprolactone and lactic acid (PCLA) scaffolds and subjecting them to shear stress provided by a pulsatile bioreactor for four days (two days at 1 dyne/cm 2 to 15 dyne/cm 2 and two days at 15 dyne/cm 2 ). Results: Shear stress significantly increased the expression of endothelial cell markers, such as platelet-endothelial cell adhesion molecule-1 (PECAM-1), VE-cadherin, and CD34, at both the mRNA and protein levels as compared with static control cells. Protein levels of alpha-smooth muscle actin (α-SMA) and calponin were substantially reduced in shear stresscultured cells. There was no significant change in the expression of α-SMA, smooth muscle myosin heavy chain (SMMHC) or calponin at the mRNA level. Conclusion: Shear stress upregulated the expression of endothelial cell-related markers and downregulated smooth muscle-related markers in canine MSCs. This study may serve as a basis for further investigation of the effects of shear stress on MSC differentiation in TEVGs.

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Section: Discussionsupporting

confidence: 93%

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

Dong

et al. 2009

Acta Pharmacol Sin

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“…Human MSC, 1x10 6 cell/rat, intra-arterially, after ureter obstruction (67). The criteria for differentiation need to be rigorously defined.…”

Section: Methodsmentioning

confidence: 99%

How do Mesenchymal Stem Cells Repair?

Semedo¹,

Burgos-Silva²,

Donizetti-Oliveira³

et al. 2011

Stem Cells in Clinic and Research

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“…For example, bone-marrow-derived MSCs from a number of species are able to differentiate into endothelial-like cells when they are stimulated through physiological shear stress conditions (Engelmayr et al, 2006;Bai et al, 2010;Huang et al, 2010;Maul et al, 2011;Dong et al, 2009;Kim et al, 2011). Likewise, MSCs derived from human adipose tissue (hASCs) (Bassaneze et al, 2010;Zhang et al, 2011;Shojaei et al, 2013) and MSCs from other sources, such as amniotic fluid cells ) and human placenta (Wu et al, 2008), have also been shown to differentiate into endothelial-like cells under shear stress (see Table 1 for a summary).…”

Section: The Effect Of Shear Stress On Endothelial Cells and Mscsmentioning

confidence: 99%

The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells

Dan

Velot

Decot

et al. 2015

Journal of Cell Science

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Mesenchymal stem cells (MSCs) are among the most promising and suitable stem cell types for vascular tissue engineering. Substantial effort has been made to differentiate MSCs towards vascular cell phenotypes, including endothelial cells and smooth muscle cells (SMCs). The microenvironment of vascular cells not only contains biochemical factors that influence differentiation, but also exerts hemodynamic forces, such as shear stress and cyclic strain. Recent evidence has shown that these forces can influence the differentiation of MSCs into endothelial cells or SMCs. In this Commentary, we present the main findings in the area with the aim of summarizing the mechanisms by which shear stress and cyclic strain induce MSC differentiation. We will also discuss the interactions between these mechanical cues and other components of the microenvironment, and highlight how these insights could be used to maintain differentiation.

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Synergism of biochemical and mechanical stimuli in the differentiation of human placenta-derived multipotent cells into endothelial cells

Cited by 83 publications

References 24 publications

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

How do Mesenchymal Stem Cells Repair?

The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells

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