Synergism of Gibberellin and Auxin in 'Delaware' Grape Ovary Development in Vitro

Shiozaki, Shuji; Ueda, Y.; Ogata, Takanari; Horiuchi, Shosaku; Kawase, Kenji

doi:10.2503/jjshs.63.703

Cited by 2 publications

(1 citation statement)

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“…In grapevine, important hormonal cascades are triggered soon after pollination, involving changes in the transcript abundance of genes associated with phytohormone biosynthesis [18][19][20][21] . Gibberellin and auxin have a berry growth promoting effect, and their addition to unpollinated pistils produces parthenocarpic berries 22,23 or promotes the setting of in vitro grown ovaries 24 . In grapevine, IAA levels are high at anthesis and decrease in coincidence with IAA-aspartate (IAA-Asp) content rise, which is an inactive auxin conjugate 25 .…”

Section: Introductionmentioning

confidence: 99%

The role of auxin during early berry development in grapevine as revealed by transcript profiling from pollination to fruit set

et al. 2021

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Auxin is a key phytohormone that modulates fruit formation in many fleshy fruits through the regulation of cell division and expansion. Auxin content rapidly increases after pollination and the manipulation in its levels may lead to the parthenocarpic development. ln Vitis vinifera L., little is known about the early fruit development that encompasses from pollination to fruit set. Pollination/fertilization events trigger fruit formation, and auxin treatment mimics their effect in grape berry set. However, the role of auxin in this process at the molecular level is not well understood. To elucidate the participation of auxin in grapevine fruit formation, morphological, reproductive, and molecular events from anthesis to fruit set were described in sequential days after pollination. Exploratory RNA-seq analysis at four time points from anthesis to fruit set revealed that the highest percentage of genes induced/repressed within the hormone-related gene category were auxin-related genes. Transcript profiling showed significant transcript variations in auxin signaling and homeostasis-related genes during the early fruit development. Indole acetic acid and several auxin metabolites were present during this period. Finally, application of an inhibitor of auxin action reduced cell number and the mesocarp diameter, similarly to unpollinated berries, further confirming the key role of auxin during early berry development. This work sheds light into the molecular features of the initial fruit development and highlights the auxin participation during this stage in grapevine.

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