Synergism of ICRF 159 and radiotherapy in treatment of experimental tumors

Hypoxic cell radiosensitizers and local control by X-ray of a transplanted tumour in mice

“…One method of overcoming the problem of hypoxic cell radioresistance is by the use of chemicals which may act, either by normalizing " the developing tumour blood vasculature (Hellmann and Murkin, 1974), or are electron-affinic compounds which mimic the radiosensitizing effect of 02, but which are not rapidlv metabolized and so can diffuse further and radiosensitize the hypoxic' cells (Adams, 1973). The present work is concerned with the use of such chemicals to overcome hypoxic cell radioresistance, using the local control (i.e.…”

“…Five compounds have been investigated: ICRF 159 which has previously been reported to " normalise " blood capillaries within tumours and so promote an improved 02 supply (Hellmann and Murkin, 1974), and 4 electron-affinic hypoxic cell radiosensitizers nifurpipone dihydrochloride, metronidazole, , which have all shown radiosensitization in vitro (Asquith et al, 1974a(Asquith et al, , 1974bChapman, Reuvers and Borsa, 1973; Adams, Asquith and 'Watts, 1974;Adams et al, 1976). the anaplastic MT tumour, arose spontaneously in a WHT/Ht mouse in 1964 in Dr Hewitt's colony at the Gray Laboratory, and has been maintained since in the same inbred strain of mice.…”

Combination therapy of a mouse sarcoma using razoxane and electron irradiation

“…The drug razoxane was made up as a fine suspension in carboxymethyl cellulose (CMC) as described by Hellmann & Murkin (1974). Drug concentrations were adjusted so that mice received i.p.…”

“…Three perpendicular measurements of tumour diameter were taken using Vernier calipers, and the product, the '"relative tumour volume" for each treatment group was plotted against time from the start of treatment, to produce a tumour growrth curve, after first relating the mean group tumour size to the mean size on the first day of treatment. On Day 28 the mean tumour volume of untreated animals was Hellmann and Murkin (1974). Three doses of razoxane were used in the present experiments: 6-25, 15 and 30 mg/kg.…”

“…Ryall et al, 1979;Spittle et al, 1979). The first report of an effect of the drug in a murine system was by Hellmann & Murkin (1974) using Sarcoma 180 and a fractionated radiation/drug regime. Razoxane alone was reported to have a "normalizing" effect on the vasculature of experimental tumours with poorly developed blood vessels (Salsbury et al, 1974).…”