Synergism of simvastatin with losartan prevents angiotensin II-induced cardiomyocyte apoptosis <i>in vitro</i>

Xu, Jian; Lu, Xuanyong; Huang, Yan; Zhu, Pengli; Li, Jun

doi:10.1211/jpp.61.04.0013

Cited by 8 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, we found a reduction in angiotensin II-mediated vasoconstriction in the aorta of the animals fed the atherogenic diet treated with simvastatin. The role of angiotensin II in mediating pulmonary fibrosis is well-established 37 , 38 , and the effect of simvastatin in reducing angiotensin II-mediated signalling is well reported 39 – 41 . Also, we found a reduction in alpha adrenergic-mediated vasoconstriction (using phenylpherine) in the aorta of the animals fed the atherogenic diet treated with simvastatin, but this did not return to normal.…”

Section: Discussionmentioning

confidence: 99%

Simvastatin impairs the induction of pulmonary fibrosis caused by a western style diet: a preliminary study

Kružliak

Hare

Zvoníček

et al. 2015

J Cellular Molecular Medi

View full text Add to dashboard Cite

The role of an atherogenic diet in causing pulmonary fibrosis has received little attention and simvastatin has been shown to reduce pulmonary fibrosis in animal models. To determine if an atherogenic diet can induce pulmonary fibrosis and whether simvastatin treatment is beneficial by up-regulating heat shock protein 70 and 90. New Zealand white rabbits (n = 15) were divided: Group 1 (control); Group 2 (MC) received a normal rabbit diet with 1% methionine plus 0.5% cholesterol (atherogenic diet). Group 3 received the same diet as the MC group plus 5 mg/kg/day simvastatin orally (MCS). After 4 weeks, the lungs were collected and analysed. Picrosirus red staining of lung interstitial collagen content showed that the atherogenic diet increased fibrosis 2.9-fold (P < 0.05), bronchiole adventitial collagen was increased 2.3-fold (P < 0.05) and bronchiole epithelium was increased 34-fold (P < 0.05), and simvastatin treatment severely reduced this effect (P < 0.05). Western blot analysis showed that the atherogenic diet significantly reduced lung Hsp70 protein by 22% (P < 0.05) and Hsp90 protein by 18% (P < 0.05) and simvastatin treatment did not affect this result. However, aortic hyper-responsiveness to vasoconstrictors (angiotensin II and phenylephrine) were markedly reduced by simvastatin treatment. We report that an atherogenic diet stimulates pulmonary fibrosis and reduces lung Hsp70/Hsp90 protein concentration. Simvastatin impairs this by mechanisms unrelated to Hsp70/Hsp90, but possibly a reduction in angiotensin II receptor or alpha adrenergic receptor pathways. These results could have implications in idiopathic pulmonary fibrosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Simvastatin impairs the induction of pulmonary fibrosis caused by a western style diet: a preliminary study

Kružliak

Hare

Zvoníček

et al. 2015

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…To detect the inhibitory effect of NGF and CNTF on extraocular muscle cells inflammatory response, after treatment, the ROS level in cells of different groups were measured by ROS detection kit following the instructions [14]. Briefly, the cells were isolated and cultured at 37°C, 5% CO 2 , followed by NGF and CNTF treatment.…”

Section: Ros Detectionmentioning

confidence: 99%

NGF and CNTF expression and regulation mechanism by miRNA in acute paralytic strabismus

Liu

Tan

et al. 2020

Int Ophthalmol

View full text Add to dashboard Cite

Nerve growth factor (NGF) and ciliary neurotrophic factor (CNTF) are well known neurotrophic factors and widely used in the clinical treatment for its promotion effect on peripheral nerve regeneration. And they were also recommended for the acute paralytic strabismus treatment. However, whether the NGF and CNTF have protective effect for the extraocular muscles of acute paralytic strabismus patients is still poorly understood. Thus, in this study, we want to evaluate the biological function of NGF and CNTF on the extraocular muscle cells and revealed the regulation mechanism behind it. Firstly, the relative expression of ngf and cntf was assessed by Quantitative Realtime Polymerase Chain Reaction (RT-PCR). Then, the influence of NGF and CNTF on the extraocular muscle cell proliferation was determined by Cell Counting Kit-8 (CCK-8). The inflammatory response in muscle cells after NGF and CNTF treatment was evaluated by ELISA and Reactive Oxygen Species (ROS) detection. In addition to this, the up-stream regulation of the ngf and cntf expression was also studied. The TargetScan was used for the predication of potential miRNAs targeting with ngf and cntf 3'-UTR, which is soon confirmed by luciferase activity assay. Taken together, all the results above indicated that NGF and CNTF could promote the muscle cells proliferation and inhibit the inflammatory levels, then exert protective effect on the muscle cells function. It was conceivable that let 7-5p was the up-stream regulator of ngf and cntf, and let 7-5p might serve as a potential molecular target for acute paralytic strabismus treatment.

show abstract

Functional Adaptation During the Development of Cardiac Hypertrophy and Heart Failure in Females

Dent

Elimban

Malik

et al. 2012

Cardiac Adaptations

View full text Add to dashboard Cite

Synergism of simvastatin with losartan prevents angiotensin II-induced cardiomyocyte apoptosis in vitro

Abstract: Our data provide the first evidence that synergism of simvastatin with losartan prevents angiotensin II-induced cardiomyocyte apoptosis in vitro. Synergism between simvastatin and losartan may provide a new therapeutic approach to the prevention of cardiac remodelling.

Cited by 8 publications

References 35 publications

Simvastatin impairs the induction of pulmonary fibrosis caused by a western style diet: a preliminary study

Simvastatin impairs the induction of pulmonary fibrosis caused by a western style diet: a preliminary study

NGF and CNTF expression and regulation mechanism by miRNA in acute paralytic strabismus

Functional Adaptation During the Development of Cardiac Hypertrophy and Heart Failure in Females

Contact Info

Product

Resources

About